Non-porous magnetic chelator supports for protein recovery by immobilised metal affinity adsorption

Journal of Biotechnology

Volumn 50, Issue 1, 1996, Pages 13-25

  O'Brien, Susan M ^a   Thomas, Owen R T ^a   Dunnill, Peter ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

cytochromes c; haemoglobin; iminodiacetic acid (IDA); immobilised metal affinity adsorptio; magnetic supports

Indexed keywords

ADSORBENTS; CHELATION; CYTOLOGY; PROTEINS; RECOVERY;

MAGNETIC CHELATOR;

BIOTECHNOLOGY;

CHELATING AGENT; COPPER ION; CYTOCHROME C; DIGLYCINE; EPOXIDE; GLUTARALDEHYDE; HEMOGLOBIN; HEMOPROTEIN; HISTIDINE; IRON OXIDE; UNCLASSIFIED DRUG;

ADSORPTION; ARTICLE; CHELATION; HYDROLYSIS; LIGAND BINDING; MAGNETISM; PH; PRIORITY JOURNAL; PROTEIN BINDING;

EID: 0030582401     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01544-1     Document Type: Article

References (26)

1. Arnold, F.H. (1991) Metal-affinity separations: a new dimension in protein processing. Bio/Technology 9, 151-156.
2. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Dunnill, P. and Lilly, M.D. (1974) Purification of enzymes using magnetic bio-affinity materials. Biotechnol. Bioeng. 16, 987-990.
4. Ennis, M.P. and Wisdom, G.B. (1991) A magnetisable solid phase for enzyme extraction. Appl. Biochem. Biotechnol. 30, 155-164.
5. Eveleigh, J.W. (1978) Techniques and instrumentation for preparative immunoadsorbent separations. J. Chromatogr. 159, 129-145.
6. Groman, E.V. and Wilchek, M. (1987) Recent developments in affinity chromatography supports. Trends Biotechnol. 5, 220-224.
7. Hailing, P.J. and Dunnill, P. (1979a) Improved nonporous magnetic supports for immobilised enzymes. Biotechnol. Bioeng. 21, 396-416.
8. Hailing, P.J. and Dunnill, P. (1979b) Recovery of free enzymes from product liquors by bio-affinity adsorption: trypsin binding by immobilised soybean inhibitor. Eur. J. Appl. Microbiol. Biotechnol. 6, 195-205.
9. Halling, P.J. and Dunnili, P. (1980) Magnetic supports for immobilised enzymes and bioaffinity adsorbents. Enzyme Microb. Technol. 2, 2-11.
10. Harwood, L.M. and Moody, C.J. (1990) Experimental Organic Chemistry - Principles and Practice, Blackwell, Oxford, UK.
11. Hemdan, E.S., Zhao, Y-J, Sulkowski, E. and Porath, J. (1989) Surface topography of histidine residues: a facile probe by immobilised metal ion affinity chromatography. Proc. Natl. Acad. Sci. USA 86, 1811-1815.
12. Hochuli, E., Dobeli, H. and Schacher, A. (1987) New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J. Chromatogr, 411, 177-184.
13. Morgan, P.E., Thomas, O.R.T., Dunnill, P., Sheppard, A.J. and Slater, N.K.H. Polyvinyl alcohol-coated perfluorocarbon supports for metal chelating affinity separation of a monoclonal antibody. J. Mol. Recognit. In press.
14. Mosbach, K. and Andersson, L. (1977) Magnetic ferrofluids for preparation of magnetic polymers and their application in affinity chromatography. Nature 270, 259-261.
15. Munro, P.A., Dunnill, P. and Lilly, M.D. (1977) Nonporous magnetic materials as enzyme supports: studies with immobilised chymotrypsin. Biotechnol. Bioeng. 19, 101-124.
16. Pitfield, I. (1992) Perfluorocarbon chromatographic supports. Ph, D. Thesis, University of Cambridge.
17. Porath, J., Carlsson, J., Olsson I. and Belfrage, G. (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258, 598-599.
18. Robinson, P.J., Dunnill, P. and Lilly, M.D. (1973) The properties of magnetic supports in relation to immobilised enzyme reactors. Biotechnol. Bioeng. 15, 603-606.
19. Setchell, C.H. (1985) Magnetic separations in biotechnology - a review. J. Chem. Tech. Biotechnol. 35B, 175-182.
20. Sundberg, L. and Porath, J. (1974) Preparation of adsorbents for biospecific affinity chromatography. I. Attachment of group-containing ligands to insoluble polymers by means of bifunctional oxiranes. J. Chromatogr. 90, 87-98.
21. Todd, R.J., Johnson, R.D. and Arnold, F.H. (1994) Multiplesite binding interactions in metal-affinity chromatography. I. Equilibrium binding of engineered histidine-containing cytochromes c. J. Chromatogr. A 662, 13-26.
22. Van Dam, M.E., Wuenschell, G.S. and Arnold, F.H. (1989) Metal affinity precipitation of proteins. Biotechnol. Appl. Biochem. 11, 492-502.
23. van Leemputten, E. and Horisberger, M. (1974) Immobilisation of enzymes on magnetic particles. Biotechnol. Bioeng. 16, 385-396.
24. Whitehead, R.A., Chagnon, M.S., Groman, E.V. and Josephson, L. (1987) U.S. Patent number 4,695,393, September 22.
25. Whitesides, G.M., Kazlaukas, R.J. and Josephson, L. (1983) Magnetic separations in biotechnology. Trends Biotechnol. 1, 144-148.
26. Wuenschell, G.E., Naranjo, E. and Arnold, F.H. (1990) Aqueous two-phase metal affinity extraction of haem proteins. Bioprocess Eng. 5, 199-202.