Two conserved cysteine residues are critical for the enzymic function of the human platelet-derived growth factor receptor-β: Evidence for different roles of Cys-822 and Cys-940 in the kinase activity

Biochemical Journal

Volumn 382, Issue 2, 2004, Pages 631-639

  Lee, Joon Won ^a   Kim, Jee Eun ^a   Park, Eun Jung ^a   Kim, Jin Hyun ^a   Lee, Chang Hun ^a   Lee, Seung Rock ^a   Kwon, Jongbum ^a  

^a Ewha Womans University   (South Korea)

Author keywords

Catalysis; Cysteine; Kinase; Mutagenesis; N ethylmaleimide; Platelet derived growth factor (PDGF)

Indexed keywords

ADENOSINETRIPHOSPHATE; BIOASSAY; BIOCHEMISTRY; CATALYSIS; CHEMICAL BONDS; CONFORMATIONS; MUTAGENESIS;

CATALYTIC ACTIVITY; CYSTEINE RESIDUES; GEL ANALYSIS; PROTEIN CONFORMATIONAL CHANGES;

ENZYME KINETICS;

ADENOSINE TRIPHOSPHATE; CYSTEINE; N ETHYLMALEIMIDE; PLATELET DERIVED GROWTH FACTOR BETA RECEPTOR; PROTEIN TYROSINE KINASE;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; CARBOXY TERMINAL SEQUENCE; CATALYSIS; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; INSECT CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; ANIMALS; CARCINOMA, HEPATOCELLULAR; CELL LINE; CELL LINE, TUMOR; CYSTEINE; CYTOPLASM; DISULFIDES; DNA, NEOPLASM; ETHYLMALEIMIDE; HUMANS; INSECTS; KIDNEY; LIVER NEOPLASMS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDES; PHOSPHOTRANSFERASES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN-TYROSINE KINASES; RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA;

ANIMALIA; INSECTA;

EID: 4544249385     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040624     Document Type: Article

References (31)

1. Heldin, C.-H., Östman, A. and Ronnstrand, L. (1997) Signal transduction via platelet-derived growth factor receptors. Biochim. Biophy, Acta 1378, F79-F113
2. Claesson-Welsh, L. (1994) Platelet-derived growth factor receptor signals. J. Biol. Chem. 269, 32023-32026
3. Baxter, R. M., Secrist, J. P., Vaillancourt, R. R. and Kazlauskas, A. (1997) Full activation of the platelet-derived growth factor beta-receptor kinase involves multiple events. J. Biol. Chem. 273, 17050-17055
4. Kazlauskas, A., Durden, D. L. and Cooper, J. A. (1991) Functions of the major tyrosine phosphorylation site of the PDGF receptor β subunit. Cell Regul. 2, 413-425
5. Fantl, W. J., Escobedo, J. A. and Williams, L. T. (1989) Mutations of the platelet-derived growth factor receptor that cause a loss of ligand-induced conformational change, subtle changes in kinase activity, and impaired ability to stimulate DNA synthesis. Mol. Cell. Biol. 9, 4473-4478
6. Rönnstrand, L., Mori, S., Arvidsson, A.-K., Eriksson, A., Wernstedt, C., Hellman, U., Claesson-Welsh, L. and Heldin, C.-H. (1992) Identifcation of two C-terminal autophosphorylatlon sites in the PDGF β-receptor: involvement in the interaction with phospholipase C-γ. EMBO J. 11, 3911-3919
7. Kashishian, A. and Cooper, J. A. (1993) Phosphorylation sites at the C-terminus of the platelet-derived growth factor receptor bind phospholipase C-γ 1. Mol. Biol. Cell 4, 49-57
8. Valius, M., Bazenet, C. and Kazlauskas, A. (1993) Tyrosines 1021 and 1009 are phosphorylation sites in the carboxy terminus of the platelet-derived growth factor receptor β subunit and are required for binding of phospholipase C-γ 1 and a 64-kilodalton protein, respectively. Mol. Cell. Biol. 13, 133-143
9. Fantl, W. J., Escobedo, J. A., Martin, G. A., Turck, C. W., del Rosario, M., McCormick, F. and Williams, L. T. (1992) Distinct phosphotyrosines on a growth factor receptor bind to specific molecules that mediate different signaling pathways. Cell (Cambridge, Mass.) 69, 413-423
10. Kazlauskas, A., Kashishian, A., Cooper, J. A. and Valius, M. (1992) GTPase-activating protein and phosphatidylinositol 3-kinase bind to distinct regions of the platelet-derived growth factor receptor β subunit. Mol. Cell. Biol. 12, 2534-2544
11. Kashishian, A., Kazlauskas, A. and Cooper, J. A. (1992) Phosphorylation sites in the PDGF receptor with different specifcities for binding GAP and PI3 kinase in vivo. EMBO J. 11, 1373-1382
12. Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell (Cambridge, Mass.) 103, 211-225
13. Huse, M. and Kuriyan, J. (2002) The conformational plasticity of protein kinases. Cell (Cambridge, Mass.) 109, 275-282
14. Hubbard, S. R. and Till, J. H. (2000) Protein tyrosine kinase structure and function. Annu. Rev. Biochem. 69, 373-398
15. Hubbard, S. R., Mohammadi, M. and Schlessinger, J. (1998) Autoregulatory mechanisms in protein-tyrosine kinases. J. Biol. Chem. 273, 11987-11990
16. Woltjer, R. L. and Staros, J. V. (1997) Effects of sulfhydryl modification reagents on the kinase activity of the epidermal growth factor receptor. Biochemistry 36, 9911-9916
17. Kato, M., Iwashita, T., Takeda, K., Akhand, A. A., Liu, W., Yoshihara, M., Asai, N., Suzuki, H., Takahashi, M. and Nakashima, I. (2000) Ultraviolet light induces redox reaction-mediated dimerization and superactivation of oncogenic ret tyrosine kinases. Mol. Biol. Cell 11, 93-101
18. lck. J. Biol. Chem. 268, 17547-17553
19. Oo, M. L., Senga, T., Thant, A. A., Amin, R. R., Huang, P., Mon, N. N. and Hamaguchi, M. (2003) Cysteine residues in the C-terminal lobe of Src: their role in the suppression of the Src kinase. Oncogene 22, 1411-1417
20. Senga, T., Miyazaki, K., Machida, K., Iwata, H., Matsuda, S., Nakashima, I. and Hamaguchi, M. (2000) Clustered cysteine residues in the kinase domain of v-Src: critical role for protein stability, cell transformation and sensitivity to herbimycin A. Oncogene 19, 273-279
21. Li, W. and Schlessinger, J. (1991) Platelet-derived growth factor (PDGF)-induced disulfide-linked dimerization of PDGF receptor in living cells. Mol. Cell. Biol. 11, 3756-3761
22. Valius, M. and Kazlauskas, A. (1993) Phospholipase C-gamma 1 and phosphatidylinositol 3 kinase are the downstream mediators of the PDGF receptor's mitogenic signal. Cell (Cambridge, Mass.) 73, 321-334
23. Lee, S.-R., Kwon, K. S., Kim, S.-R. and Rhee, S. G. (1998) Reversible inactivation of protein-tyrosine phosphatase 1B in A431 cells stimulated with epidermal growth factor. J. Biol. Chem. 273, 15366-15372
24. Jensen, R. A., Beeler, J. F., Heidaran, M. A. and LaRochelle, W. J. (1992) Characterization of baculovirus-expressed human α and β platelet-derived growth factor receptors. Biochemistry 31, 10887-10892
25. 2. J. Biol. Chem. 277, 20336-20342
26. Xu, D., Rovira, I. I. and Finkel, T. (2002) Oxidants painting the cysteine chapel: redox regulation of PTPs. Dev. Cell 2, 251-252
27. Denu, J. M. and Dixon, J. E. (1998) Protein tyrosine phosphatases: mechanisms of catalysis and regulation. Curr. Opin. Chem. Biol. 2, 633-641
28. Rhee, S. G., Bae, Y. S., Lee, S. R. and Kwon, J. (2000) Hydrogen peroxide: a key messenger that modulates protein phosphorylation through cysteine oxidation. Science STKE 53, PE1
29. 2 production requires the activation of phosphatidylinositol 3-kinase. J. Biol. Chem. 275, 10527-10531
30. 2 for platelet-derived growth factor signal transduction. Science 270, 296-299
31. McTigue, M. A., Wickersham, J. A., Pinko, C., Showalter, R. E., Parast, C. V., Tempczyk-Russell, A., Gehring, M. R., Mroczkowski, B., Kan, C.-C., Villafranca, J. E. et al. (1999) Crystal structure of the kinase domain of human vascular endothelial growth factor receptor 2: a key enzyme in angiogenesis. Structure Fold. Des. 7, 319-339