Profiling primary protease specificity by peptide synthesis on a solid support

Angewandte Chemie - International Edition

Volumn 43, Issue 24, 2004, Pages 3138-3141

  Doezé, Ron H P ^a   Maltman, Beatrice A ^a   Egan, Claire L ^a   Ulijn, Rein V ^b   Flitsch, Sabine L ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Enzymes; Fluorescent probes; High throughput screening; Hydrolysis; Substrate specificity

Indexed keywords

AMINO ACIDS; CATALYSIS; ENZYMES; FLUORESCENCE; HYDROLYSIS;

PEPTIDE HYDROLYSIS; PROTEASE-CATALYZED COUPLING;

POLYPEPTIDES;

AMINO ACID DERIVATIVE; PEPTIDE; PROTEINASE;

ANALYTIC METHOD; ARTICLE; CATALYSIS; CHEMICAL REACTION; FLUORESCENCE; HIGH THROUGHPUT SCREENING; HYDROLYSIS; MONITORING;

ACRYLAMIDES; AMINO ACIDS; BACILLUS; BIOCOMPATIBLE MATERIALS; PEPTIDE HYDROLASES; PEPTIDES; POLYETHYLENE GLYCOLS; POLYMERS; SUBSTRATE SPECIFICITY; THERMOLYSIN;

EID: 4544236023     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200353367     Document Type: Article

References (16)

1. N. D. Rawlings, E. O'Brien, A. J. Barrett, Nucleic Acids Res. 2002, 50, 343-346.
2. a) D. J. Maly, L. Huang, J. A. Ellman, ChemBioChem 2002, 3, 16-37;
3. b) B. B. Davis, D. A. Thompson, L. L. Howard, C. Morisseau, B. D. Hammock, R. H. Weiss, Proc. Natl. Acad. Sci. USA 2002, 99, 2222-2227.
4. F. Bordusa, Chem. Rev. 2002, 102, 4817-4867.
5. a) P. M. St. Hilaire, L. C. Alves, F. Herrera, M. Renil, S. J. Sanderson, J. C. Mottram, G. H. Coombs, M. A. Juliano, L. Juliano, J. Arevalo, M. Meldal, J. Med. Chem. 2002, 45, 1971-1982;
6. b) J. E. Sheppeck, H. Kar, L. Gosink, J. B. Wheatley, E. Gjerstad, S. M. Loftus, A. R. Zubiria, J. W. Jane, Bioorg. Med. Chem. Lett. 2000, 10, 2639-2642;
7. c) M. Meldal, Biopolymers 2002, 66, 93-100.
8. a) C. M. Salisbury, D. J. Maly, J. A. Ellman, J. Am. Chem. Soc. 2002, 124, 14868-14870;
9. b) Q. Zhu, M. Uttamchandani, D. Li, M. L. Lesaicherre, S. Q. Yao, Org. Lett. 2003, 5, 1257-1260;
10. c) G. Y. J. Chen, M. Uttamchandani, R. Y. P. Lue, M. L. Lesaicherre, S. Q. Yao, Curr. Top. Med. Chem. 2003, 3, 705-724.
11. I. Schechter, A. Berger, Biochem. Biophys. Res. Commun. 1967, 27, 157-162.
12. a) R. V. Ulijn, B. Baragana, P. J. Hailing, S. L. Putsch, J. Am. Chem. Soc. 2002, 124, 10988-10989;
13. b) R. V. Ulijn, N. Bisek, P. J. Hailing, S. L. Flitsch, Org. Biomol. Chem. 2003, 1, 1277-1281.
14. A. Y. Bosma, R. V. Ulijn, G. McConnell, J. Girkin, P. J. Hailing, S. L. Flitsch, Chem. Commun. 2003, 22, 2790-2791.
15. For more details on preferential cleavage sites and further references, see http://www.chem.qmul.ac.uk/iubmb/enzyme/EC3/4/21/1.html and http://www.chem.qmul.ac.uk/iubmb/enzyme/EC3/4/24/27.html.
16. D. M. Blow, Acc. Chem. Res. 1976, 9, 145-152.