A simple, chisel-assisted mechanical microdissection system for harvesting homogenous plant tissue suitable for the analysis of nucleic acids and proteins

Plant Molecular Biology Reporter

Volumn 21, Issue 4, 2003, Pages 417-427

  Brandt, Stephan ^a   Walz, Christina ^b   Schad, Martina ^b   Pavlovic, Nada ^c   Kehr, Julia ^b  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

^c Eppendorf Vertrieb Deutschland GmbH   (Germany)

Author keywords

Mass spectrometry; Mechanical microdissection; Paraffin embedding; Protein sequencing; RT PCR; Single cell analysis; Tissue specificity

Indexed keywords

EID: 4544235270     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02772591     Document Type: Article

References (23)

1. Arlt K, Brandt S, and Kehr J (2001) Amino acid analysis in five pooled single plant cell samples using capillary electrophoresis coupled to laser-induced fluorescence detection. J Chomatogr A 926: 319-325.
2. Asano T, Masumura T, Kusano H, Kikuchi S, Kurita A, Shimada H, and Kadowaki KI (2002) Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem. Plant J 32: 401-408.
3. Brandt S, Kehr J, Walz C, Imlau A, Willmitzer L, and Fisahn J (1999) A rapid method for detection of plant gene transcripts from single epidermal, mesophyll and companion cells of intact leaves. Plant J 20: 245-250.
4. Brandt S, Kloska S, Altmann T, and Kehr J (2002) Using array hybridization to monitor gene expression at the single cell level. J Exp Bot 53, 2315-2323.
5. Emmert-Buck M, Bonner R, Smith P, Chuaqui R, Zhuang Z, Goldstein S, Weiss R, and Liotta L (1996) Laser capture microdissection. Science 274: 998-1001.
6. Fricke W, Leigh R, and Tomos D (1994) Concentrations of inorganic and organic solutes in extracts from individual epidermal, mesophyll and bundle-sheath cells of barley leaves. Planta 192: 310-316.
7. -14 moles of sucrose in plant tissue. Plant Physiol 60: 379-383.
8. Kehr J, Wagner C, Willmitzer L, and Fisahn J (1999) Effect of modified carbon allocation on turgor, osmolality, sugar and potassium content, and membrane potential in the epidermis of transgenic potato (Solanum tuberosum L.) plants. J Exp Bot 50: 565-571.
9. Kehr J (2001) High resolution spatial analysis of plant systems. Curr Opin Plant Biol 4: 197-201.
10. Kehr J (2003) Single cell technology. Curr Opin Plant Biol 6: 1-5.
11. Kerk NM, Ceserani T, Tausta SL, Sussex IM, and Nelson TM (2003) Laser capture microdissection of cells from plant tissues. Plant Physiol 132: 27-35.
12. Lochmann H, Bazzanella A, and Bächmann K (1998) Analysis of solutes and metabolites in single plant cell vacuoles by capillary electrophoresis. J Chromatogr A 817: 337-343.
13. Nakazono M, Qiu F, Borsuk LA, and Schnable PS (2003) Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: identification of genes expressed differentially in epidermal cells or vascular tissues of maize. Plant Cell 15: 583-596.
14. 14C in the tissues following pulse labelling. Plant Physiol 55: 699-703.
15. Outlaw W and Lowry O (1977) Organic acid and potassium acculmulation in guard cells during stomatal opening. Proc Natl Acad Sci USA 74: 4434-4438.
16. -12 mol of potassium by stimulation of pyruvate kinase. Anal Biochem 92: 370-379.
17. Outlaw W (1980) A descriptive evaluation of quantitative histochemical methods based on pyridine nucleotides. Ann Rev Plant Physiol 31: 299-311.
18. Rekhter MD and Chen J (2001) Molecular analysis of complex tissues is facilitated by laser capture microdissection. Cell Biochem Biophys 35: 103-113.
19. Shevchenko A, Jensen O, Podtelejnikov A, Sagliocco F, Wilm M, Vorm O, Mortensen P, Shevchenko A, Boucherie H, and Mann M (1996) Linking genome and proteome by mass spectrometry-large scale identification of yeast proteins from two dimensional gels. Proc Natl Acad Sci USA 93: 14440-14445.
20. Tomos D, Hinde P, Richardson P, Pritchard J, and Fricke W (1994) Microsampling and measurements of solutes in single cells. In: Harris N and Oparka K (eds) Plant Cell Biology-A practical approach, pp 297-314, Oxford University Press Oxford.
21. Tomos D and Sharrock R (2001) Cell sampling and analysis (SiCSA): metabolites measured at single cell resolution. J Exp Bot 52: 623-630.
22. Wittliff J, Kunitake S, Chu S, and Travis J (2000) Applications of laser capture microdissection in genomics and proteomics. J Clin Ligand Assay 23: 66-73.
23. Ziegler B, Lamping C, Thoma S, and Thomas C (1992) Single cell cDNA- PCR: Removal of contaminating genomic DNA from total RNA using immobilized DNAse I. Bio-Techniques 13: 726-729.