Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: Toward comprehensive analysis of the genes expressed in the rice phloem

Plant Journal

Volumn 32, Issue 3, 2002, Pages 401-408

  Asano, Takayuki ^a,b   Masumura, Takehiro ^c   Kusano, Hiroaki ^a,b   Kikuchi, Shoshi ^a   Kurita, Akihiro ^c   Shimada, Hiroaki ^b   Kadowaki, Koh Ichi ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b TOKYO UNIVERSITY OF SCIENCE   (Japan)

^c KYOTO PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Cell specific; Laser capture microdissection; Phloem specific; Plant; RNA polymerase amplification

Indexed keywords

CELLS; CLONING; DNA; RNA; TISSUE; TUMORS;

HYBRIDIZATION;

PLANTS (BOTANY);

AMINO ACID TRANSPORT SYSTEMS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA-DIRECTED RNA POLYMERASES; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, PLANT; GENE LIBRARY; IN SITU HYBRIDIZATION; LASERS; ORYZA SATIVA; PLANT COMPONENTS; PLANT LEAVES; RNA, PLANT; SEQUENCE HOMOLOGY, NUCLEIC ACID;

EMBRYOPHYTA; EUKARYOTA; MAMMALIA;

EID: 0036845188     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2002.01423.x     Document Type: Article

References (15)

1. Asano, T., Kunieda, N., Omura, Y., Ibe, H., Kawasaki, T., Takano, M., Sato, M., Furuhashi, H., Mujin, T., Takaiwa, F., Wu, C.-Y., Tada, Y., Satozawa, T., Sakamoto, M. and Shimada, H. (2002) Rice SPK, a calmodulin-like domain protein kinase, is required for storage product accumulation during seed development: Phosphorylation of sucrose synthase is a possible factor. Plant Cell 14, 619-628.
2. Balachandran, S., Xiang, Y., Schobert, C., Thompson, G.A. and Lucas, W.J. (1997) Phloem sap proteins from Cucurbita maxima and Ricinus communis have the capacity to traffic cell to cell through plasmodesmata. Proc. Natl Acad. Sci. USA 94, 14150-14155.
3. Bonner, R.F., Emmert-Buck, M., Cole, K., Pohida, T., Chuaqui, R., Goldstein, S. and Liotta, L.A. (1997) Laser capture microdissection: Molecular analysis of tissue. Science 278, 1481-1483.
4. Emmert-Buck, M.R., Bonner, R.F., Smith, P.D., Chuaqui, R.F., Zhuang, Z., Goldstein, S.R., Weiss, R.A. and Liotta, L.A. (1996) Laser capture microdissection. Science 274, 998-1001.
5. +-cotransporters for cellular import of neutral and charged amino acids. Plant J. 29, 717-731.
6. Goldsworthy, S.M., Stockton, P.S., Trempus, C.S., Foley, J.F. and Maronpot, R.R. (1999) Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol. Carcinog. 25, 86-91.
7. Ishiwatari, Y., Fujiwara, T., McFarland, K.C., Nemoto, K., Hayashi, H., Chino, M. and Lucas, W.J. (1998) Rice phloem thioredoxin h has the capacity to mediate its own cell-to-cell transport through plasmodesmata. Planta 205, 12-22.
8. Kadowaki, K., Kubo, N., Ozawa, K. and Hirai, A. (1996) Targeting presequence acquisition after mitochondrial gene transfer to the nucleus occurs by duplication of existing targeting signals. EMBO J. 15, 6652-6661.
9. Kouchi, H. and Hata, S. (1993) Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development. Mol. Gen. Genet. 238, 106-119.
10. Lee, D., Ellard, M., Wanner, L.A., Davis, K.R. and Douglas, C.J. (1995) The Arabidopsis thaliana 4-coumarate: CoA ligase (4CL) gene: Stress and developmentally regulated expression and nucleotide sequence of its cDNA. Plant Mol. Biol. 28, 871-884.
11. Luo, L., Salunga, R.C., Guo, H., Bittner, A., Joy, K.C., Galindo, J.E., Xiao, H., Rogers, K.E., Wan, J.S., Jackson, M.R. and Erlander, M.G. (1999) Gene expression profiles of laser-captured adjacent neuronal subtypes. Nature Med. 5, 117-122.
12. Miyao, A., Yamazaki, M. and Hirochika, H. (1998) Systematic screening of mutants of rice by sequencing retrotransposon-insertion sites. Plant Biotechn. 15, 253-256.
13. Peterson, L.A., Brown, M.R., Carlisle, A.J., Kohn, E.C., Liotta, L.A., Emmert-Buck, M.R. and Krizman, D.B. (1998) An improved method for construction of directionally cloned cDNA libraries from microdissected cells. Cancer Res. 58, 5326-5328.
14. Scheidl, S.J., Nilsson, S., Kalén, M., Hellström, M., Takemoto, M., Hakansson, J. and Lindahl, P. (2002) mRNA expression profiling of laser microbeam microdissected cells from slender embryonic structures. Am. J. Pathol. 160, 801-813.
15. Szederkényi, J., Komor, E. and Schobert, C. (1997) Cloning of the cDNA for glutaredoxin, an abundant sieve-tube exudate protein from Ricinus communis L. & characterisation of the glutathione-dependent thiol-reduction system in sieve tubes. Planta 202, 349-356.