Influence of droplet size, capillary-cone distance and selected instrumental parameters for the analysis of noncovalent protein-ligand complexes by nano-electrospray ionization mass spectrometry

Journal of Mass Spectrometry

Volumn 39, Issue 9, 2004, Pages 1059-1067

  Benkestock, Kurt ^a,b   Sundqvist, Gustav ^b   Edlund, Per Olof ^c   Roeraade, Johan ^b  

^a BIOVITRUM AB   (Sweden)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^c NONE   (Sweden)

Author keywords

Capillary cone distance; Droplet size; H FABP; Nano electrospray ionization; Noncovalent complexes; RNAse

Indexed keywords

COMPLEXATION; DISSOCIATION; HYDROPHILICITY; IONIZATION; PROTEINS; SPRAYING;

ELECTROSPRAY IONIZATION (ESI); FREE PROTEIN RATIO;

MASS SPECTROMETRY;

BUFFER; CYTIDINE PHOSPHATE; CYTIDINE TRIPHOSPHATE; LIGAND; PROTEIN; RIBONUCLEASE;

ARTICLE; CHEMICAL BOND; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DEVICE; DISSOCIATION; ELECTROSPRAY; ELECTROSPRAY MASS SPECTROMETRY; FLOW RATE; GAS FLOW; HYDROPHILICITY; HYDROPHOBICITY; IONIZATION; MOLECULAR STABILITY; PARAMETER; PRIORITY JOURNAL; SAMPLE; SAMPLING; STRUCTURE ANALYSIS; TEMPERATURE;

ANIMALS; CARRIER PROTEINS; ELECTROLYTES; FATTY ACID-BINDING PROTEINS; HEART; HORSES; IONS; LIGANDS; MYOGLOBIN; NANOTECHNOLOGY; PARTICLE SIZE; PROTEINS; RIBONUCLEASE, PANCREATIC; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 4544221115     PISSN: 10765174     EISSN: None     Source Type: Journal    
DOI: 10.1002/jms.685     Document Type: Article

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