AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans

Biochemical Journal

Volumn 412, Issue 3, 2008, Pages 485-493

  Robellet, Xavier ^a   Flipphi, Michel ^b   Pégot, Sylvine ^a   MacCabe, Andrew P ^b   Vélot, Christian ^a  

^a INSTITUTE FOR INTEGRATIVE BIOLOGY OF THE CELL I2BC   (France)

^b INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

Author keywords

Acetate; Aspergillus nidulans; Gene expression; GPR1 FUN34 YaaH protein family; Monocarboxylate transport

Indexed keywords

ASPERGILLUS; BIOLOGICAL MEMBRANES; ENCODING (SYMBOLS); GENE EXPRESSION;

ACETATE; ASPERGILLUS NIDULANS; FUNCTIONAL CHARACTERIZATION; HYPOTHETICAL PROTEIN; MONOCARBOXYLATE TRANSPORT; PROTEIN FAMILY; SOLE CARBON SOURCE; TRANSCRIPTIONAL ACTIVATORS;

PROTEINS;

ACETIC ACID; ACETIC ACID ETHYL ESTER; ALCOHOL; CARBON 14; PERMEASE;

ARTICLE; ASPERGILLUS NIDULANS; CARBON SOURCE; CELL PH; CONIDIUM; CONTROLLED STUDY; ENZYME ACTIVITY; FUNGAL STRAIN; GENE DELETION; GENE STRUCTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTON TRANSPORT;

EMERICELLA NIDULANS; FUNGI;

EID: 45349103446     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20080124     Document Type: Article

References (49)

1. Felenbok, B., Flipphi, M. and Nikolaev, I. (2001) Ethanol catabolism in Aspergillus nidulans: a model system for studying gene regulation. Prog. Nucleic Acid Res. Mol. Biol. 69, 149-204
2. Sandeman, R. A. and Hynes, M. J. (1989) Isolation of the facA (acetyl-coenzyme A synthetase) and acuE (malate synthase) genes of Aspergillus nidulans. Mol. Gen. Genet. 218, 87-92
3. Armitt, S., McCullough, W. and Roberts, C. F. (1976) Analysis of acetate non-utilizing (acu) mutants in Aspergillus nidulans. J. Gen. Microbiol. 92, 263-282
4. Ballance, D. J. and Turner, G. (1986) Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD). Mol. Gen. Genet. 202, 271-275
5. Todd, R. B., Murphy, R. L., Martin, H. M., Sharp, J. A., Davis, M. A., Katz, M. E. and Hynes, M. J. (1997) The acetate regulatory gene facB of Aspergillus nidulans encodes a Zn(II)2Cys6 transcriptional activator. Mol. Gen. Genet. 254, 495-504
6. Fillinger, S. and Felenbok, B. (1996) A newly identified gene cluster in Aspergillus nidulans comprises five novel genes localized in the alc region that are controlled both by the specific transactivator AlcR and the general carbon-catabolite repressor CreA. Mol. Microbiol. 20, 475-488
7. Flipphi, M., Robellet, X., Dequier, E., Leschelle, X., Felenbok, B. and Vélot, C. (2006) Functional analysis of alcS, a gene of the alc cluster in Aspergillus nidulans. Fungal Genet. Biol. 43, 247-260
8. Kujau, M., Weber, H. and Barth, G. (1992) Characterization of mutants of the yeast Yarrowia lipolytica defective in acetyl-coenzyme A synthetase. Yeast 8, 193-203
9. Rabitsch, K. P., Tóth, A., Gálová, M., Schleiffer, A., Schaffner, G., Aigner, E., Rupp, C., Penkner, A. M., Moreno-Borchart, A. C., Primig, M. et al. (2001) A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr. Biol. 11, 1001-1009
10. Stettler, S., Mariotte, S., Riva, M., Sentenac, A. and Thuriaux, P. (1992) An essential and specific subunit of RNA polymerase III (C) is encoded by gene RPC34 in Saccharomyces cerevisiae. J. Biol. Chem. 267, 21390-21395
11. Paiva, S., Devaux, F., Barbosa, S., Jacq, C. and Casal, M. (2004) Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae. Yeast 21, 201-210
12. Augstein, A., Barth, K., Gentsch, M., Kohlwein, S. D. and Barth, G. (2003) Characterization, localization and functional analysis of Gpr1p, a protein affecting sensitivity to acetic acid in the yeast Yarrowia lipolytica. Microbiology 149, 589-600
13. Gentsch, M., Kuschel, M., Schlegel, S. and Barth, G. (2007) Mutations at different sites in members of the Gpr1/Fun34/YaaH protein family cause hypersensitivity to acetic acid in Saccharomyces cerevisiae as well as in Yarrowia lipolytica. FEMS Yeast Res. 7, 380-390
14. Galagan, J. E., Calvo, S. E., Cuomo, C., Ma, L. J., Wortman, J. R., Batzoglou, S., Lee, S. I., Bastürkmen, M., Spevak, C. C., Clutterbuck, J. et al. (2005) Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438, 1105-1115
15. Clutterbuck, A. J. (1993) Aspergillus nidulans. In Genetic Maps: Locus Maps of Complex Genomes, vol. 3 (O'Brien, S. J., ed.), pp. 3.71-3.84, Cold Spring Harbor Laboratory Press, New York
16. Cove, D. J. (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim. Biophys. Acta 113, 51-56
17. Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York
18. Specht, C. A., DiRusso, C. C., Novotny, C. P. and Ullrich, R. C. (1982) A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal. Biochem. 119, 158-163
19. Clutterbuck, A. J. (1974) Aspergillus nidulans. In Handbook of Genetics. Volume 1: Bacteria, Bacteriophages, and Fungi (King, R. C., ed.), pp. 447-510, Plenum Press, New York
20. Tilburn, J., Scazzocchio, C., Taylor, G. G., Zabicky-Zissman, J. H., Lockington, R. A. and Davies, R. W. (1983) Transformation by integration in Aspergillus nidulans. Gene 26, 205-221
21. Yu, J. H., Hamari, Z., Han, K. H., Seo, J. A., Reyes-Dominguez, Y. and Scazzocchio, C. (2004) Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet. Biol. 41, 973-981
22. Kurtov, D., Kinghorn, J. R. and Unkles, S. E. (1999) The Aspergillus nidulans panB gene encodes ketopantoate hydroxymethyltransferase, required for biosynthesis of pantothenate and Coenzyme A. Mol. Gen. Genet. 262, 115-120
23. Forment, J. V., Flipphi, M., Ramón, D., Ventura, L. and MacCabe, A. P. (2006) Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans. J. Biol. Chem. 281, 8339-8346
24. Flipphi, M., Kocialkowska, J. and Felenbok, B. (2003) Relationships between the ethanol utilization (alc) pathway and unrelated catabolic pathways in Aspergillus nidulans. Eur. J. Biochem. 270, 3555-3564
25. Flipphi, M., van de Vondervoort, P. J. I., Ruijter, G. J. G., Visser, J., Arst, Jr, H. N. and Felenbok, B. (2003) Onset of carbon catabolite repression in Aspergillus nidulans. Parallel involvement of hexokinase and glucokinase in sugar signaling. J. Biol. Chem. 278, 11849-11857
26. MacCabe, A. P., Miró, P., Ventura, L. and Ramón, D. (2003) Glucose uptake in germinating Aspergillus nidulans conidia: involvement of the creA and sorA genes. Microbiology 149, 2129-2136
27. Flipphi, M., Kocialkowska, J. and Felenbok, B. (2002) Characteristics of physiological inducers of the ethanol utilization (alc) pathway in Aspergillus nidulans. Biochem. J. 364, 25-31
28. Todd, R. B., Kelly, J. M., Davis, M. A. and Hynes, M. J. (1997) Molecular characterization of mutants of the acetate regulatory gene facB of Aspergillus nidulans. Fungal Genet. Biol. 22, 92-102
29. Todd, R. B., Andrianopoulos, A., Davis, M. A. and Hynes, M. J. (1998) FacB, the Aspergillus nidulans activator of acetate utilization genes, binds dissimilar DNA sequences. EMBO J. 17, 2042-2054
30. Kim, H., Melén, K. and von Heijne, G. (2003) Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions. J. Biol. Chem. 278, 10208-10213
31. Daley, D. O., Rapp, M., Granseth, E., Melén, K., Drew, D. and von Heijne, G. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308, 1321-1323
32. Casal, M., Cardoso, H. and Leão, C. (1996) Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae. Microbiology 142, 1385-1390
33. Casal, M. and Leão, C. (1995) Utilization of short-chain monocarboxylic acids by the yeast Torulaspora delbrueckii: specificity of the transport systems and their regulation. Biochim. Biophys. Acta 1267, 122-130
34. Semighini, C. P., Goldman, M. H. and Goldman, G. H. (2004) Multi-copy suppression of an Aspergillus nidulans mutant sensitive to camptothecin by a putative monocarboxylate transporter. Curr. Microbiol 49, 229-233
35. Makuc, J., Paiva, S., Schauen, M., Krämer, R., André, B., Casal, M., Leão, C. and Boles, E. (2001) The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane. Yeast 18, 1131-1143
36. Kupfer, D. M., Drabenstot, S. D., Buchanan, K. L., Lai, H., Zhu, H., Dyer, D. W., Roe, B. A. and Murphy, J. W. (2004) Introns and splicing elements of five diverse fungi. Eukaryotic Cell 3, 1088-1100
37. Prade, R. A. and Timberlake, W. E. (1993) The Aspergillus nidulans brlA regulatory locus consists of overlapping transcription units that are individually required for conidiophore development. EMBO J. 12, 2439-2447
38. Han, S., Navarro, J., Greve, R. A. and Adams, T. H. (1993) Translational repression of brlA expression prevents premature development in Aspergillus. EMBO J. 12, 2449-2457
39. Casal, M., Paiva, S., Andrade, R. P., Gancedo, C. and Leão, C. (1999) The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1. J. Bacteriol. 181, 2620-2633
40. Andrade, R. P., Kötter, P., Entian, K. D. and Casal, M. (2005) Multiple transcripts regulate glucose-triggered mRNA decay of the lactate transporter JEN1 from Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 332, 254-262
41. Borsuk, P., Dzikowska, A., Empel, J., Grzelak, A., Grzeskowiak, R. and Weglenski, P. (1999) Structure of the arginase coding gene and its transcript in Aspergillus nidulans. Acta Biochim. Pol. 46, 391-403
42. Borsuk, P., Przykorska, A., Blachnio, K., Koper, M., Pawlowicz, J. M., Pekala, M. and Weglenski, P. (2007) L-Arginine influences the structure and function of arginase mRNA in Aspergillus nidulans. Biol. Chem. 388, 135-144
43. Cássio, F., Leão, C. and van Uden, N. (1987) Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae. Appl. Environ. Microbiol. 53, 509-513
44. Andrade, R. P. and Casal, M. (2001) Expression of the lactate permease gene JEN1 from the yeast Saccharomyces cerevisiae. Fungal Genet. Biol. 32, 105-111
45. Flipphi, M., Mathieu, M., Cirpus, I., Panozzo, C. and Felenbok, B. (2001) Regulation of the aldehyde dehydrogenase gene (aldA) and its role in the control of the coinducer level necessary for induction of the ethanol utilization pathway in Aspergillus nidulans. J. Biol. Chem. 276, 6950-6958
46. Apirion, D. (1965) The two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in Aspergillus nidulans. Genet. Res. 6, 317-329
47. von Heijne, G. (1992) Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J. Mol. Biol. 225, 487-494
48. Thompson, J. D., Higgins, D. G. and Gibson, T. J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680
49. Felsenstein, J. (1989) PHYLIP - Phylogeny Inference Package (Version 3.2). Cladistics 5, 164-166