Rapid identification of the three major species of dairy obligate heterofermenters Lactobacillus brevis, Lactobacillus fermentum and Lactobacillus parabuchneri by species-specific duplex PCR

FEMS Microbiology Letters

Volumn 284, Issue 2, 2008, Pages 150-157

  Coton, Monika ^a   Berthier, Françoise ^b   Coton, Emmanuel ^a  

^a Adria Normandie ^*  (France)

^b CEMAGREF   (France)

Author keywords

Dairy products; Identification; Obligately heterofermentative lactobacilli; Species specific primers

Indexed keywords

RNA 16S; RNA 23S;

ARTICLE; BACTERIUM; BACTERIUM IDENTIFICATION; CHEESE; CONTROLLED STUDY; DAIRY PRODUCT; FERMENTATION; GENE CLUSTER; GENE SEQUENCE; GENOTYPE; LACTIC ACID BACTERIUM; LACTOBACILLUS; LACTOBACILLUS BREVIS; LACTOBACILLUS DIOLIVORANS; LACTOBACILLUS FERMENTUM; LACTOBACILLUS PARABUCHNERI; LACTOBACILLUS REUTERI; LEUCONOSTOC; MICROBIAL DIVERSITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PEDIOCOCCUS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTOCOCCUS; WEISELLA;

BACTERIAL TYPING TECHNIQUES; BIODIVERSITY; CHEESE; DNA FINGERPRINTING; DNA, BACTERIAL; DNA, RIBOSOMAL SPACER; ELECTROPHORESIS, AGAR GEL; FERMENTATION; FOOD MICROBIOLOGY; LACTOBACILLUS BREVIS; LACTOBACILLUS FERMENTUM; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY;

LACTOBACILLUS; LACTOBACILLUS BREVIS; LACTOBACILLUS DIOLIVORANS; LACTOBACILLUS FERMENTUM; LACTOBACILLUS PARABUCHNERI; LACTOBACILLUS REUTERI; LACTOBACILLUS SP.; LEUCONOSTOC; PEDIOCOCCUS; STREPTOCOCCUS; WEISELLA;

EID: 44949178440     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2008.01206.x     Document Type: Article

References (30)

1. Aquilanti L, Dell'Aquila L, Zannini E, Zocchetti A Clementi F (2006) Resident lactic acid bacteria in raw milk Canestrato Pugliese cheese. Lett Appl Microbiol 43 : 161 167.
2. Berthier F, Beuvier E, Dasen A Grappin R (2001) Origin and diversity of mesophilic lactobacilli in Comté cheese, as revealed by PCR with repetitive and species-specific primers. Int Dairy J 11 : 293 305.
3. Crow V, Curry B Hayes M (2001) The ecology of non-starter lactic acid bacteria (NSLAB) and their use as adjuncts in New Zealand Cheddar. Int Dairy J 11 : 275 283.
4. Demarigny Y, Beuvier E, Dasen A Duboz G (1996) Influence of raw milk microflora on the characteristics of Swiss-type cheeses. I. Evolution of microflora during ripening and characterization of facultatively heterofermentative lactobacilli. Lait 76 : 371 387.
5. Gevers D, Huys G Swings J (2001) Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205 : 31 36.
6. Gobbetti M, Folkertsma B, Fox PF, Corsetti A, Smacchi E, De Angelis M, Rossi J, Kilcawley K Cortini M (1999) Microbiology and biochemistry of Fossa (pit) cheese. Int Dairy J 9 : 763 773.
7. Jordan KN Cogan TM (1993) Identification and growth of non-starter lactic acid bacteria in Irish Cheddar cheese. Ir J Agri Food Res 32 : 47 55.
8. Kabadjova P, Dousset X, Le Cam V Prevost H (2002) Differentiation of closely related Carnobacterium food isolates based on 16S-23S ribosomal DNA intergenic spacer region polymorphism. Appl Environ Microbiol 68 : 5358 5366.
9. Lazzi C, Rossetti L, Zago M, Neviani E Giraffa G (2004) Evaluation of bacterial communities belonging to natural whey starters by length heterogeneity-PCR. J Appl Microbiol 96 : 481 490.
10. Leuschner RG, Kurihara R Hammes WP (1998) Effect of enhanced proteolysis on formation of biogenic amines by lactobacilli during Gouda cheese ripening. Int J Food Microbiol 44 : 15 20.
11. Lynch CM, Muir DD, Banks JM McSweeney PL (1999) Influence of adjunct cultures of Lactobacillus paracasei spp. paracasei or Lactobacillus plantarum on cheddar cheese ripening. J Dairy Sci 82 : 1618 1628.
12. Novella-Rodriguez S, Veciana-Nogues MT, Roig-Sagues AX, Trujillo-Mesa AJ Vidal-Carou MC (2004) Evaluation of biogenic amines and microbial counts throughout the ripening of goat cheeses from pasteurized and raw milk. J Dairy Res 71 : 245 252.
13. Ouadghiri M, Amar M, Vancanneyt M Swings J (2005) Biodiversity of lactic acid bacteria in Moroccan soft white cheeses (Jben). FEMS Microbiol Lett 251 : 267 271.
14. Perry KD McGillivray WA (1964) The manufacture of normal and starter-free cheddar cheese under controlled bacteriological conditions. J Dairy Res 31 : 155 165.
15. Sanchez I, Sesena S, Poveda JM, Cabezas L Palop L (2006) Genetic diversity, dynamics, and activity of Lactobacillus community involved in traditional processing of artisanal Manchego cheese. Int J Food Microbiol 107 : 265 273.
16. Settanni L, van Sinderen D, Rossi J Corsetti A (2005) Rapid differentiation and in situ detection of 16 sourdough Lactobacillus species by multiplex PCR. Appl Environ Microbiol 71 : 3049 3059.
17. Skeie S, Lindberg C Narvhus J (2001) Development of amino acids and organic acids in Norvegia, influence of milk treatment and adjunct Lactobacillus. Int Dairy J 11 : 399 411.
18. Stiles ME Holzapfel WH (1997) Review article: lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36 : 1 29.
19. Sumner SS, Speckhard MW, Somers EB Taylor SL (1985) Isolation of histamine-producing Lactobacillus buchneri from Swiss cheese implicated in a food poisoning outbreak. Appl Environ Microbiol 50 : 1094 1096.
20. Swearingen PA, O'Sullivan DJ Warthesen JJ (2001) Isolation, characterization, and influence of native, nonstarter lactic acid bacteria on Cheddar cheese quality. J Dairy Sci 84 : 50 59.
21. Taylor SL, Keefe TJ, Windham ES Howell JF (1982) Outbreak of histamine poisoning associated with consumption of Swiss cheese. J Food Prot 45 : 455 457.
22. Tungjaroenchai W, Drake MA White CH (2001) Influence of adjunct cultures on ripening of reduced fat Edam cheeses. J Dairy Sci 84 : 2117 2124.
23. Van Camp G, Chapelle S De Watcher R (1993) Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences. Curr Microbiol 27 : 147 151.
24. Vancanneyt M, Engelbeen K, De Watcher M, Vandemeulebroecke K, Cleenwerck I Swings J (2005) Reclassification of Lactobacillus ferintoshensis as a later heterotypic synonym of Lactobacillus parabuchneri. Int J Syst Evol Microbiol 55 : 2195 2198.
25. Vancanneyt M, Naser SM, Engelbeen K, De Watcher M, Van der Meulen R, Cleenwerck I, Hoste B, De Vuyst L Swings J (2006) Reclassification of Lactobacillus brevis strains LMG 11494 and LMG 11984 as Lactobacillus parabrevis sp. nov. Int J Syst Evol Microbiol 56 : 1553 1557.
26. Versalovic J, Koeuth T Lupski JR (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19 : 6823 6831.
27. Versalovic J, Schneider G, Bruijn FJ Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). Methods Mol Cell Biol 5 : 25 40.
28. Wijesundera C, Roberts M Limsowtin GKY (1997) Flavour development in aseptic cheese curd slurries prepared with single-strain starter bacteria in the presence and absence of adjuncts. Lait 77 : 121 131.
29. Wilmotte A, Van der Auwera G De Watcher R (1993) Structure of the 16S ribosomal RNA of the thermophilic cynobacterium Chlorogloeopsis HTF ('Mastigocladus laminosus HTF') strain PCC7518, and phylogenetic analysis. FEBS Lett 317 : 96 100.
30. Wouters JTM, Ayad EHE, Hugenholtz J Smit G (2002) Microbes from raw milk for fermented dairy products. Int Dairy J 12 : 91 109.