Application of a novel in vitro selection technique to isolate and characterise high affinity DNA aptamers binding mammalian prion proteins

Journal of Virological Methods

Volumn 151, Issue 1, 2008, Pages 107-115

  Bibby, David F ^a   Gill, Andrew C ^b   Kirby, Louise ^b   Farquhar, Christine F ^b   Bruce, Moira E ^b   Garson, Jeremy A ^a,c  

^a Windeyer Institute   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c UNIVERSITY COLLEGE LONDON HOSPITALS   (United Kingdom)

Author keywords

DNA aptamer; Prion protein; PrPSc; SELEX; Transmissible spongiform encephalopathy

Indexed keywords

APTAMER; DNA; FLUORESCEIN; GUANIDINE; ISOPROTEIN; LIGAND; NICKEL COMPLEX; PRION PROTEIN; SINGLE STRANDED DNA;

ANIMAL CELL; ARTICLE; COMBINATORIAL CHEMISTRY; DENATURATION; DISSOCIATION CONSTANT; DNA BINDING; DNA ISOLATION; FLOW CYTOMETRY; IN VITRO STUDY; MAGNET; MAMMAL; MOLECULAR CLONING; MOUSE; NONHUMAN; PRION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN IMMOBILIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; APTAMERS, NUCLEOTIDE; BASE SEQUENCE; CLONING, MOLECULAR; FLOW CYTOMETRY; HUMANS; MICE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PRIONS; PROTEIN BINDING; PRPC PROTEINS; PRPSC PROTEINS; RECOMBINANT PROTEINS; SELEX APTAMER TECHNIQUE; SHEEP;

MAMMALIAN PRION; MURINAE; OVIS;

EID: 44649202665     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.03.013     Document Type: Article

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