Standardized quantitative RT-PCR assays for quantitation of yellow fever and chimeric yellow fever-dengue vaccines

Journal of Virological Methods

Volumn 151, Issue 1, 2008, Pages 40-46

  Mantel, N ^a   Aguirre, M ^a   Gulia, S ^a   Girerd Chambaz, Y ^a   Colombani, S ^a   Moste, C ^a   Barban, V ^a  

^a SANOFI PASTEUR   (France)

Author keywords

Dengue; Quantitative RT PCR; Yellow fever

Indexed keywords

DENGUE VACCINE; YELLOW FEVER VACCINE;

ANTIBODY TITER; ARTICLE; CHIMERA; DENGUE VIRUS; MONKEY; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA TRANSCRIPTION; VIRUS ISOLATION; YELLOW FEVER FLAVIVIRUS;

ANIMALS; CERCOPITHECUS AETHIOPS; DENGUE; DENGUE VACCINES; DENGUE VIRUS; DNA PRIMERS; MACACA FASCICULARIS; PLAQUE ASSAY; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VACCINES, SYNTHETIC; VERO CELLS; VIRAL ENVELOPE PROTEINS; VIRAL NONSTRUCTURAL PROTEINS; YELLOW FEVER; YELLOW FEVER VACCINE; YELLOW FEVER VIRUS;

EID: 44649126660     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.03.026     Document Type: Article

References (21)

1. Anderson K.B., Chunsuttiwat S., Nisalak A., Mammen M.P., Libraty D.H., Rothman A.L., Green S., Vaughn D.W., Ennis F.A., and Endy T.P. Burden of symptomatic dengue infection in children at primary school in Thailand: a prospective study. Lancet 369 9571 (2007) 1452-1459
2. Bae H.G., Nitsche A., Teichmann A., Biel S.S., and Niedrig M. Detection of yellow fever virus: a comparison of quantitative real-time PCR and plaque assay. J. Virol. Methods 110 (2003) 185-191
3. Barban V., Girerd Y., Aguirre M., Gulia S., Petiard F., Riou P., Barrere B., and Lang J. High stability of yellow fever 17D-204 vaccine: a 12-year restrospective analysis of large-scale production. Vaccine 25 (2007) 2941-2950
4. Chao D.Y., Davis B.S., and Chang G.J. Development of multiplex real-time reverse transcriptase PCR assays for detecting eight medically important flaviviruses in mosquitoes. J. Clin. Microbiol. 45 (2007) 584-589
5. Chien L.J., Liao T.L., Shu P.Y., Huang J.H., Gubler D.J., and Chang G.J. Development of real-time reverse transcriptase PCR assays to detect and serotype dengue viruses. J. Clin. Microbiol. 44 (2006) 1295-12304
6. Drosten C., Gottig S., Schilling S., Asper M., Panning M., Schmitz H., and Gunther S. Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J. Clin. Microbiol. 40 (2002) 2323-2330
7. Edelman R. Dengue and dengue vaccines. J. Infect. Dis. 191 (2005) 650-653
8. Guirakhoo F., Arroyo J., Pugachev K.V., Miller C., Zhang Z.X., Weltzin R., Georgakopoulos K., Catalan J., Ocran S., Soike K., Ratterree M., and Monath T.P. Construction, safety, and immunogenicity in nonhuman primates of a chimeric yellow fever-dengue virus tetravalent vaccine. J. Virol. 75 (2001) 7290-7304
9. Guirakhoo F., Pugachev K., Zhang Z., Myers G., Levenbrook I., Draper K., Lang J., Ocran S., Mitchell F., Parsons M., Brown N., Brandler S., Fournier C., Barrere B., Rizvi F., Travassos A., Nichols R., Trent D., and Monath T.P. Safety and efficacy of chimeric yellow fever-dengue virus tetravalent vaccine formulations in non-human primates. J. Virol. 78 (2004) 4761-4775
10. Guirakhoo F., Kitchener S., Morrison D., Forrat R., McCarthy K., Nichols R., Yoksan S., Duan X., Ermak T.H., Kanesa-Thasan N., Bedford P., Lang J., Quentin-Millet M.J., and Monath T.P. Live attenuated chimeric yellow fever dengue type 2 (ChimeriVax-DEN2) vaccine: phase I clinical trial for safety and immunogenicity: effect of yellow fever pre-immunity in induction of cross neutralizing antibody responses to all 4 dengue serotypes. Hum. Vac. 2 (2006) 60-67
11. Johnson B.W., Russel B.J., and Lanciotti R.S. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J. Clin. Microbiol. 43 (2005) 4977-4983
12. Kong Y.Y., Thay C.H., Tin T.C., and Devi S. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. J. Virol. Methods 138 (2006) 123-130
13. Lai Y.L., Chung Y.K., Tan H.C., Yap H.F., Yap G., Ooi E.E., and Ng L.C. Cost-effective real-time reverse transcriptase PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J. Clin. Microbiol. 45 (2007) 935-941
14. Lindenbach B.D., and Rice C.M. Flaviviridae: the viruses and their replication. Fields Virology. 4th ed. (2001), Lippincott Williams & Wilkins pp. 991-1042
15. Pierre V., Drouet M.T., and Deubel V. Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction. Res. Virol. 145 (1994) 93-104
16. Porterfield J.S. A plaque technique for the titration of yellow fever virus and antisera. Trans. R. Soc. Trop. Med. Hyg. 53 (1959) 458-466
17. Sambrook J., and Russel D.W. Molecular Cloning: A Laboratory Manual. 3rd edition (2001), CSHL Press
18. Simasathien S., and Watanaveeradej V. Dengue vaccine. J. Med. Assoc. Thai. 88 Suppl. 3 (2005) S363-S377
19. Shu P.Y., Chang S.F., Kuo Y.C., Yueh Y.Y., Chien L.J., Sue C.L., Lin T.H., and Huang J.H. Development of group- and serotype-specific one-step SYBR green I-based real-time reverse transcription-PCR assay for dengue virus. J. Clin. Microbiol. 41 (2003) 2408-2416
20. Theiler, M., Smith, H.H., 2000. The use of yellow fever virus modified by in vitro cultivation for human immunization. J. Exp. Med. 65, 787-800 (1937) (Rev. Med. Virol. 10, 6-16).
21. Westaway E.G., Brinton M.A., Gaidamovich S.Y., Horzinek M.C., Igarashi A., Kaariainen L., Lvov D.K., Porterfield J.S., Russell P.K., and Trent D.W. Flaviviridae. Intervirology 24 (1985) 183-192