Dexamethasone blocks hypoxia-induced endothelial dysfunction in organ-cultured pulmonary arteries

American Journal of Respiratory and Critical Care Medicine

Volumn 170, Issue 6, 2004, Pages 647-655

  Murata, Takahisa ^a   Hori, Masatoshi ^a   Sakamoto, Kenichi ^a   Karaki, Hideaki ^a   Ozaki, Hiroshi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Dexamethasone; Endothelium dependent relaxation; Hypoxia; Organ culture

Indexed keywords

CALCIUM IONOPHORE; CALCIUM SALT; CALMODULIN BINDING PROTEIN; CAVEOLIN 1; CYCLIC GMP; DEXAMETHASONE; ENDOTHELIAL NITRIC OXIDE SYNTHASE; IONOMYCIN; MESSENGER RNA; NITROPRUSSIDE SODIUM; PROSTAGLANDIN F2 ALPHA; PROTEIN KINASE B; PROTEIN SERINE THREONINE KINASE; SUBSTANCE P;

ANIMAL TISSUE; ARTERY CONSTRICTION; ARTICLE; ATROPHY; CALCIUM CELL LEVEL; DRUG INHIBITION; DRUG MECHANISM; ENDOTHELIUM CELL; HYPOXIA; LUNG ENDOTHELIUM; MALE; MUSCLE TONE; NONHUMAN; ORGAN CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PULMONARY ARTERY; RABBIT; REAL TIME POLYMERASE CHAIN REACTION; VASCULAR DISEASE; VASCULAR ENDOTHELIUM; VASCULAR SMOOTH MUSCLE; WESTERN BLOTTING;

ANIMALS; ANOXIA; ATROPHY; CELLS, CULTURED; DEXAMETHASONE; ENDOTHELIUM, VASCULAR; GLUCOCORTICOIDS; MALE; NITRIC OXIDE SYNTHASE; PULMONARY ARTERY; RABBITS; VASCULAR DISEASES;

EID: 4444334342     PISSN: 1073449X     EISSN: None     Source Type: Journal    
DOI: 10.1164/rccm.200309-1311OC     Document Type: Article

References (34)

1. Hampl V, Herget J. Role of nitric oxide in the pathogenesis of chronic pulmonary hypertension. Physiol Rev 2000;80:1337-1372.
2. Xue C, Johns RA. Endothelial nitric oxide synthase in the lungs of patients with pulmonary hypertension. N Engl J Med 1995;333:1642-1644.
3. Tobin MJ. Chronic obstructive pulmonary disease, pollution, pulmonary vascular disease, transplantation, pleural disease, and lung cancer in AJRCCM 2003. Am J Respir Crit Care Med 2004;169:301-313.
4. Ghofrani HA, Schermuly RT, Rose F, Wiedemann R, Kohstall MG, Kreckel A, Olschewski H, Weissmann N, Enke B, Ghofrani S, et al. Sildenafil for long-term treatment of nonoperable chronic thromboembolic pulmonary hypertension. Am J Respir Crit Care Med 2003;167:1139-1141.
5. Kuhn KP, Byrne DW, Arbogast PG, Doyle TP, Loyd JE, Robbins IM. Outcome in 91 consecutive patients with pulmonary arterial hypertension receiving epoprostenol. Am J Respir Crit Care Med 2003;167:580-586.
6. Rudic RD, Shesely EG, Maeda N, Smithies O, Segal SS, Sessa WC. Direct evidence for the importance of endothelium-derived nitric oxide in vascular remodeling. J Clin Invest 1998;101:731-736.
7. Maeda Y, Ikeda U, Oya K, Shimpo M, Ueno S, Okada K, Saito T, Mano H, Ozawa K, Shimada K. Endogenously generated nitric oxide by nitric-oxide synthase gene transfer inhibits cellular proliferation. J Pharmacol Exp Ther 2000;292:387-393.
8. Govers R, Rabelink TJ. Cellular regulation of endothelial nitric oxide synthase. Am J Physiol Renal Physiol 2001;280:F193-F206.
9. Sessa WC, Garcia-Cardena G, Liu J, Keh A, Pollock JS, Bradley J, Thiru S, Braverman IM, Desai KM. The Golgi association of endothelial nitric oxide synthase is necessary for the efficient synthesis of nitric oxide. J Biol Chem 1995;270:17641-17644.
10. Gratton JP, Fontana J, O'Connor DS, Garcia-Cardena G, McCabe TJ, Sessa WC. Reconstitution of an endothelial nitric-oxide synthase (eNOS), hsp90, and caveolin-1 complex in vitro: evidence that hsp90 facilitates calmodulin stimulated displacement of eNOS from caveolin-1. J Biol Chem 2000;275:22268-22272.
11. Fulton D, Gratton JP, McCabe TJ, Fontana J, Fujio Y, Walsh K, Franke TF, Papapetropoulos A, Sessa WC. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 1999;399:597-601.
12. Fleming I, Fisslthaler B, Dimmeler S, Kemp BE, Busse R. Phosphorylation of Thr(495) regulates Ca(2+)/calmodulin-dependent endothelial nitric oxide synthase activity. Circ Res 2001;88:E68-E75.
13. Murata T, Sato K, Hori M, Ozaki H, Karaki H. Decreased endothelial nitric-oxide synthase (eNOS) activity resulting from abnormal interaction between eNOS and its regulatory proteins in hypoxia-induced pulmonary hypertension. J Biol Chem 2002;277:44085-44092.
14. Murata T, Yamawaki H, Hori M, Sato K, Ozaki H, Karaki H. Hypoxia impairs endothelium-dependent relaxation in organ cultured pulmonary artery. Eur J Pharmacol 2001;421:45-53.
15. Wang Y, Zhang L, Shen W, Chen L, Fan S. Interrelation between nitric oxide and endothelin-1 in an experimental acute hypoxia in rats and its intervention. Chin Med J (Engl) 1999;112:363-365.
16. Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM. Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 1985;318:635-641.
17. Messmer UK, Winkel G, Briner VA, Pfeilschifter J. Glucocorticoids potently block tumour necrosis factor-alpha- and lipopolysaccharide-induced apoptotic cell death in bovine glomerular endothelial cells upstream of caspase 3 activation. Br J Pharmacol 1999;127:1633-1640.
18. Suzuki Y, Nishio K, Takeshita K, Takeuchi O, Watanabe K, Sato N, Naoki K, Kudo H, Aoki T, Yamaguchi K. Effect of steroid on hyperoxia-induced ICAM-1 expression in pulmonary endothelial cells. Am J Physiol Lung Cell Mol Physiol 2000;278:L245-L252.
19. Wheller SK, Perretti M. Dexamethasone inhibits cytokine-induced intercellular adhesion molecule- 1 up-regulation on endothelial cell lines. Eur J Pharmacol 1997;331:65-71.
20. Murata T, Yamawaki H, Yoshimoto R, Hori M, Sato K, Ozaki H, Karaki H. Chronic effect of doxorubicin on vascular endothelium assessed by organ culture study. Life Sci 2001;69:2685-2695.
21. 2+-calmodulin and caveolin. J Biol Chem 1997;272:15583-15586.
22. Garcia-Cardena G, Oh P, Liu J, Schnitzer JE, Sessa WC. Targeting of nitric oxide synthase to endothelial cell caveolae via palmitoylation: implications for nitric oxide signaling. Proc Natl Acad Sci USA 1996;93:6448-6453.
23. Garcia-Cardena G, Fan R, Shah V, Sorrentino R, Cirino G, Papapetropoulos A, Sessa WC. Dynamic activation of endothelial nitric oxide synthase by Hsp90. Nature 1998;392:821-824.
24. Shirai M, Pearson JT, Shimouchi A, Nagaya N, Tsuchimochi H, Ninomiya I, Mori H. Changes in functional and histological distributions of nitric oxide synthase caused by chronic hypoxia in rat small pulmonary arteries. Br J Pharmacol 2003;139:899-910.
25. Le Cras TD, Xue C, Rengasamy A, Johns RA. Chronic hypoxia upregulates endothelial and inducible NO synthase gene and protein expression in rat lung. Am J Physiol 1996;270:L164-L170.
26. Shaul PW, North AJ, Brannon TS, Ujiie K, Wells LB, Nisen PA, Lowenstein CJ, Snyder SH, Star RA. Prolonged in vivo hypoxia enhances nitric oxide synthase type I and type III gene expression in adult rat lung. Am J Respir Cell Mol Biol 1995;13:167-174.
27. Limbourg FP, Huang Z, Plumier JC, Simoncini T, Fujioka M, Tuckermann J, Schutz G, Moskowitz MA, Liao JK. Rapid nontranscriptional activation of endothelial nitric oxide synthase mediates increased cerebral blood flow and stroke protection by corticosteroids. J Clin Invest 2002; 110:1729-1738.
28. Gruetter CA, Gruetter DY, Lyon JE, Kadowitz PJ, Ignarro LJ. Relationship between cyclic guanosine 3′:5′-monophosphate formation and relaxation of coronary arterial smooth muscle by glyceryl trinitrate, nitroprusside, nitrite and nitric oxide: effects of methylene blue and methemoglobin. J Pharmacol Exp Ther 1981;219:181-186.
29. Moore TM, Chetham PM, Kelly JJ, Stevens T. Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am J Physiol 1998;275:L203-L222.
30. Busse R, Edwards G, Feletou M, Fleming I, Vanhoutte PM, Weston AH. EDHF: bringing the concepts together. Trends Pharmacol Sci 2002;23:374-380.
31. Kirsch EA, Yuhanna IS, Chen Z, German Z, Sherman TS, Shaul PW. Estrogen acutely stimulates endothelial nitric oxide synthase in H441 human airway epithelial cells. Am J Respir Cell Mol Biol 1999;20:658-666.
32. MacRitchie AN, Jun SS, Chen Z, German Z, Yuhanna IS, Sherman TS, Shaul PW. Estrogen upregulates endothelial nitric oxide synthase gene expression in fetal pulmonary artery endothelium. Circ Res 1997;81:355-362.
33. Bonnet S, Dumas-de-La-Roque E, Begueret H, Marthan R, Fayon M, Dos Santos P, Savineau JP, Baulieu EE. Dehydroepiandrosterone (DHEA) prevents and reverses chronic hypoxic pulmonary hypertension. Proc Natl Acad Sci USA 2003;100:9488-9493.
34. Resta TC, Kanagy NL, Walker BR. Estradiol-induced attenuation of pulmonary hypertension is not associated with altered eNOS expression. Am J Physiol Lung Cell Mol Physiol 2001;280:L88-L97.