Chronic hypoxia upregulates endothelial and inducible NO synthase gene and protein expression in rat lung

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 270, Issue 1 14-1, 1996, Pages

  Le Cras, Timothy D ^a   Xue, Chun ^a   Rengasamy, Appavoo ^a   Johns, Roger A ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

endothelial nitric oxide synthase; inducible nitric oxide synthase; nitric oxide; pulmonary hypertension

Indexed keywords

NITRIC OXIDE; NITRIC OXIDE SYNTHASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ENDOTHELIUM; GENE EXPRESSION; HYPOXIA; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; LUNG BLOOD VESSEL; LUNG HOMOGENATE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; PULMONARY HYPERTENSION; RAT; RECEPTOR UPREGULATION; RESISTANCE BLOOD VESSEL; SHEAR STRESS;

EID: 0030023029     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.1996.270.1.l164     Document Type: Article

References (33)

1. Adnot, S., B. Raffestin, S. Eddahibi, P. Braquet, and P. Chabrier. Loss of endothelium-dependent relaxant activity in the pulmonary circulation of rats exposed to chronic hypoxia. J. Clin. Invest. 87: 155-162, 1991.
2. Archer, S., V. Hampl, Z. McKenzie, D. Nelson, J. Huang, P. J. Shultz, and E. K. Weir. Role of endothelial-derived nitric oxide in normal and hypertensive pulmonary vasculature. Semin. Respir. Crit. Care Med. 15: 179-189, 1994.
3. Chomczynski, P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 15: 532-537, 1993.
4. Chu, S. C., H. Wu, T. C. Banks, N. T. Eissa, and J. Moss. Structural diversity in the 5′-untranslated region of cytokine-stimulated human inducible nitric oxide synthase mRNA. J. Biol. Chem. 270: 10625-10630, 1995.
5. Dinh-Xuan, A. T. Disorders of endothelium-dependent relaxation in pulmonary disease. Circulation 87: V81-V87, 1993.
6. Eddahibi, S., S. Adnot, C. Carville, Y. Blouquit, and B. Raffestin. L-Arginine restores endothelium-dependent relaxation in pulmonary circulation of chronically hypoxic rats. Am. J. Physiol. 263 (Lung Cell. Mol. Physiol. 7): L194-L200, 1992.
7. Feinberg, A. P., and B. Vogelstein. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6-13, 1983. [Addendum. Anal. Biochem. 137: 266-267, 1984.]
8. Garg, U. C., and A. Hassid. Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells. J. Clin. Invest. 83: 1774-1777, 1989.
9. Ghezzi, P., C. A. Dinarello, S. Bianchi, M. E. Rosandich, J. E. Repine, and C. W. White. Hypoxia increases production of interleukin-1 and tumor necrosis factor by human mononuclear cells. Cytokines 3: 189-194, 1991.
10. Hampl, V., D. N. Cornfield, N. J. Cowan, and S. L. Archer. Hypoxia potentiates nitric oxide synthesis and transiently increases cytosolic calcium levels in pulmonary artery endothelial cells. Eur. Respir. J. 8: 515-522, 1995.
11. Hassid, A., H. Arabshahi, T. Bourcier, G. S. Phaursi, and C. Matthews. Nitric oxide selectively amplifies FGF-2-induced mitogenesis in primary rat aortic smooth muscle cells. Am. J. Physiol. 267 (Heart Circ. Physiol. 36): H1040-H1048, 1994.
12. Isaacson, T. C., V. Hampl, E. K. Weir, D. P. Nelson, and S. L. Archer. Increased endothelium-derived NO in hypertensive pulmonary circulation of chronically hypoxic rats. J. Appl. Physiol. 76: 933-940, 1994.
13. Johns, R. A., J. M. Linden, and M. J. Peach. Endothelium-dependent relaxation and cyclic GMP accumulation in rabbit pulmonary artery are selectively impaired by moderate hypoxia. Circ. Res. 65: 1508-1515, 1989.
14. Karakurum, M., R. Shreeniwas, J. Chen, D. Pinsky, S. D. Yan, M. Anderson, K. Sunouchi, J. Major, T. Hamilton, and K. Kuwabara. Hypoxic induction of interleukin-8 gene expression in human endothelial cells. J. Clin. Invest. 93: 1564-1570, 1994.
15. Kobzik, L., D. S. Bredt, C. J. Lowenstein, J. Drazen, B. Gaston, D. Sugarbaker, and J. S. Stamler. Nitric oxide synthase in human and rat lung: immunocytochemical and histochemical localization. Am. J. Respir. Cell Mol. Biol. 9: 371-377, 1993.
16. Kourembanas, S., R. L. Hannan, and D. V. Faller, Oxygen tension regulates the expression of the platelet-derived growth factor-B chain gene in human endothelial cells. J. Clin. Invest. 86: 670-674, 1990.
17. Kourembanas, S., P.A. Marsden, L. P. McQuillan, and D. V. Faller. Hypoxia induces endothelin gene expression and secretion in cultured human endothelium. J. Clin. Invest. 88: 1054-1057, 1991.
18. Le Cras, T. D., G. C. Dailey, and R. A. Johns. Endothelial NO synthase mRNA and protein, and inducible NO synthase mRNA are upregulated in the rat lung following chronic hypoxia (Abstract). Am. J. Respir. Crit. Care Med. 151: A729, 1995.
19. Maxwell, P. H., C. W. Pugh, and P. J. Ratcliffe. Inducible operation of the erythropoietin 3′ enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. Proc. Natl. Acad. Sci. USA 90: 2423-2427, 1993.
20. McQuillan, L. P., G. K. Leung, P. A. Marsden, S. K. Kostyk, and S. Kourembanas. Hypoxia inhibits expression of eNOS via transcriptional and posttranscriptional mechanisms. Am. J. Physiol. 267 (Heart Cire. Physiol. 36): H1921-H1927, 1994.
21. Morris, S. M., and T. R. Billiar. New insights into the regulation of inducible nitric oxide synthesis. Am. J. Physiol. 266 (Endocrinol. Metab. 29): E829-E839, 1994.
22. Nathan, C. Nitric oxide as a secretory product of mammalian cells. FASEB J. 6: 3051-3064, 1992.
23. North, A. J., R. A. Star, T. S. Brannon, K. Ujiie, L. B. Wells, C. J. Lowenstein, S. H. Snyder, and P. W. Shaul. Nitric oxide synthase type I and type III gene expression are developmentally regulated in rat lung. Am. J. Physiol. 266 (Lung Cell. Mol. Physiol. 10): L635-L641, 1994.
24. Rengasamy, A., and R. A. Johns. Characterization of endothelium-derived relaxing factor/nitric oxide synthase from bovine cerebellum and mechanism of modulation by high and low oxygen tensions. J. Pharmacol. Exp. Ther. 259: 310-316, 1991.
25. Roos, C. M., D. U. Frank, C. Xue, R. A. Johns, and G. F. Rich. Chronic inhaled nitric oxide: effects on pulmonary endothelial function and pathology in hypoxic and normoxic rats. J. Appl. Physiol. 80: 252-260, 1996.
26. Russ, R. D., and B. R. Walker. Maintained endothelium-dependent pulmonary vasodilation following chronic hypoxia in the rat. J. Appl. Physiol. 74: 339-344, 1993.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. Molecular Cloning: A Laboratory Manual (2nd ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.
28. Sessa, W. C., J. K. Harrison, C. M. Barber, D. Zeng, M. E. Durieux, D. D. D'Angelo, K. R. Lynch, and M. J. Peach. Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase. J. Biol. Chem. 267: 15274-15276, 1992.
29. Sessa, W. C., K. Pritchard, N. Seyedi, J. Wang, and T. H. Hintze. Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression. Circ. Res. 74: 349-353, 1994.
30. Shaul, P. W., A. J. North, T. S. Brannon, K. Ujiie, L. B. Wells, P. A. Nisen, C. J. Lowenstein, S. H. Snyder, and R. A. Star. Prolonged in vivo hypoxia enhances nitric oxide synthase type I and type III gene expression in adult rat lung. Am. J. Respir. Cell Mol. Biol. 13: 167-174, 1995.
31. Tracey, W. R., C. Xue, V. Klinghofer, J. Barlow, J. S. Pollock, U. Forstermann, and R. A. Johns. Immunochemical detection of inducible NO synthase in human lung. Am. J. Physiol. 266 (Lung Cell. Mol. Physiol. 10): L722-L727, 1994.
32. Xie, Q., H. J. Cho, J. Calaycay, R. A. Mumford, K. M. Swiderek, T. D. Lee, A. Ding, T. Troso, and C. Nathan. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science Wash. DC 256: 225-228, 1992
33. Xue, C., A. Rengasamy, T. D. Le Cras, P. A. Koberna, G. C. Dailey, and R. A. Johns. Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia Am. J. Physiol. 267 (Lung Cell. Mol. Physiol. 11): L667-L678, 1994.