A mutation study of catalytic residue Asp 52 in hen egg lysozyme

Journal of Biochemistry

Volumn 119, Issue 1, 1996, Pages 145-150

  Hashimoto, Yoshio ^a   Yamada, Keiko ^a   Motoshima, Hiroyuki ^a   Omura, Tadahiro ^a   Yamada, Hidenori ^a   Yasukochi, Takanori ^a   Miki, Takeyoshi ^a   Ueda, Tadashi ^a   Imoto, Taiji ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Catalytic mechanism; Expression system; Hen lysozyme; Mutagenesis; X ray analysis

Indexed keywords

ASPARTIC ACID; BETA FRUCTOFURANOSIDASE; LYSOZYME; SIGNAL PEPTIDE;

ARTICLE; CATALYSIS; EGG; ENZYME ACTIVE SITE; ENZYME RELEASE; EXPRESSION VECTOR; HEN; HYDROLYSIS; MUTAGENESIS; NONHUMAN; PH; YEAST;

EID: 0030047236     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021199     Document Type: Article

References (31)

1. Miki, T., Yasukouchi, T., Nagatani, H., Furuno, M., Orita, T., Yamada, H., Imoto, T., and Horiuchi, T. (1987) Construction of a plasmid vector for the regulatable high level expression of eukaryotic genes in Escherichia coli: An application to overproduction of chicken lysozyme. Protein Eng. 1, 327-332
2. Bitter, G.A., Chen, K.K., Banks, A.R., and Lai, P.H. (1984) Secretion of foreign proteins from Saccharomyces cerevisiae directed by α-factor gene fusions. Proc. Natl. Acad. Sci. USA 81, 5330-5334
3. Miyanohara, A., Toh-e, A., Nozaki, C., Hamada, F., Ohtomo, N., and Matsubara, K. (1983) Expression of hepatitis B surface antigen gene in yeast. Proc. Natl. Acad. Sci. USA 80, 1-5
4. Oberto, J. and Davison, J. (1985) Expression of chicken egg white lysozyme by Saccharomyces cerevisiae. Gene 40, 57-65
5. Thim, L., Hansen, M.T., Norris, K., Hoegh, I., Boel, E., Forstrom, J., Ammerer, G., and Fiil, N.P. (1986) Secretion and processing of insulin precursors in yeast. Proc. Natl. Acad. Sci. USA 83, 6766-6770
6. Kumagai, I., Kojima, S., Tamaki, E., and Miura, K. (1987) Conversion of Trp 62 of hen egg-white lysozyme to Tyr by site-directed mutagenesis. J. Biochem. 102, 733-740
7. Jigami, I., Muraki, M., Harada, N., and Tanaka, H. (1986) Expression of synthetic human-lysozyme gene in Saccharomyces cerevisiae: Use of a synthetic chicken-lysozyme signal sequence for secretion and processing. Gene 43, 273-279
8. Yamamoto, Y., Taniyama, Y., Kikuchi, M., and Ikehara, M. (1987) Engineering of the hydrophobic segment of the signal sequence for efficient secretion of human lysozyme by Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 149, 431-436
9. Imoto, T., Johnson, L.N., North, A.C.T., Phillips, D.C., and Rupley, J. A. (1972) Lysozyme in Enzymes (Boyer, P.D., ed.) Vol. 7, 3rd ed., pp. 665-868, Academic Press, New York
10. Blake, C.C.F., Johnson, L.N., Mair, G.A., North, A.C.T., Phillips, D.C., and Sarma, V. (1967) Crystallographic studies on the activity of hen egg white lysozyme. Proc. Roy. Soc. Ser. B 167, 378-388
11. Kuroki, R., Yamada, H., Moriyama, T., and Imoto, T. (1986) Chemical mutations of the catalytic carboxyl groups in lysozyme to the corresponding amides. J. Biol Chem. 261, 13571-13574
12. Malcolm, B.A., Rosenberg, S., Corey, M.J., Allen, J.S., Baetselier, A.D., and Kirsch, J.F. (1989) Site-directed mutagenesis of the catalytic residues Asp-52 and Glu-35 of chicken egg white lysozyme. Proc. Natl. Acad. Sci. USA 86, 133-137
13. Lumb, K.J., Aplin, R.T., Radford, S.E., Archer, D.J., Jeenes, D.J., Lambert, N., MacKenzie, D.A., Dobson, C.M., and Lowe, G. (1992) A study of D52S hen lysozyme-GlcNAc oligosaccharide complexes by NMR spectroscopy and electrospray mass spectrometry. FEBS Lett. 296, 153-157
14. Hadfield, A.T., Harvey, D.J., Archer, D.B., MacKenzie, D.A., Jeenes, D.J., Radford, S.E., Lowe, G., Dobson, C.M., and Johnson, L.N. (1994) Crystal structure of the mutant D52S hen egg white lysozyme with an oligosaccharide product. J. Mol. Biol. 243, 856-872
15. Weaver, L.H., Grutter, M.G., and Matthews, B.W. (1995) The refined structures of goose lysozyme and its complex with a bound trisaccharide show that the "goose-type" lysozymes lack a catalytic aspartate residue. J. Mol. Biol. 245, 54-68
16. Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heyneker, H.L., Boyer, H.W., Crosa, J.H., and Falkow, S. (1977) Construction and characterization of new cloning vehicles. Gene 2, 95-113
17. Vanish-Perron, C., Vieira, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13 mp18 and pUC 19 vector. Gene 33, 103-119
18. Hinnen, A., Hicks, J.B., and Fink, G.R. (1978) Transformation of yeast. Proc. Natl. Acad. Sci. USA 76, 1929-1933
19. Maki, S., Kuribatashi, M., Miki, T., and Horiuchi, T. (1983) An amber replication mutant of F plasmid mapped in the minimal replication region. Mol. Gen. Genet. 191, 231-237
20. Toh-e, A., Ueda, Y., Kakimoto, S., and Oshima, Y. (1973) Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae. J. Bacteriol. 113, 727-738
21. Imoto, T., Yamada, H., Yasukochi, T., Yamada, E., Ito, Y., Ueda, T., Nagatani, H., Miki, T., and Horiuchi, T. (1987) Point mutation of alanine (31) to valine prohibits the folding of reduced lysozyme by sulfhydryl-disulfide interchange. Protein Eng. 1, 333-338
22. Miyanohara, A., Toh-e, A., Nozaki, C., Hamada, F., Ohtomo, N., and Matsubara, K. (1983) Expression of hepatitis B surface antigen gene in yeast. Proc. Natl. Acad. Sci. USA 80, 1-5
23. Vieira, J. and Messing, J. (1987) Production of single-stranded plasmid DNA. Methods Enzymol. 153, 3-11
24. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
25. Miki, T., Easton, A.M., and Rownd, R.H. (1978) Mapping of the resistance genes of the R plasmid NRl. Mol. Gen. Genet. 158, 217-224
26. Kunkel, T.A., Roberts, J.D., and Zakour, R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 254, 367-382
27. Ito, Y., Yamada, H., and Imoto, T. (1992) Colorimetric assay for lysozyme using Micrococcus luteus labeled with a blue dye, remazol brilliant Blue R, as a substrate. Chem. Pharm. Bull. 40, 1523-1526
28. a of glutamic acid-35. Biochemistry 31, 5545-5553
29. Chang, C.N., Matteucci, M., Perry, L.J., Wulf, J.J., Chen, C.Y., and Hitzeman, R.A. (1986) Saccharomyces cerevisiae secretes and correctly processes human interferon hybrid proteins con-taining yeast invertase signal peptides. Mol. Cell. Biol. 6, 1812-1819
30. Nakamura, Y., Sato, T., Emi, M., Miyanohara, A., Nishida, T., and Matsubara, K. (1986) Expression of human salivary alpha-amylase gene in Saccharomyces cerevisiae and its secretion using the mammalian signal sequence. Gene 50, 239-245
31. Shigematsu, Y., Miyata, T., Higashi, S., Miki, T., Sadler, J.E., and Iwanaga, S. (1992) Expression of human soluble tissue factor in yeast and enzymatic properties of its complex with factor VIIa. J. Biol. Chem. 267, 21329-21337