Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A 2 in the monomeric and dimeric states: Insights into its oligomeric state

Biochemical and Biophysical Research Communications

Volumn 323, Issue 1, 2004, Pages 24-31

  Magro, Angelo J ^a   Murakami, Mário T ^a   Marcussi, Silvana ^b   Soares, Andreimar M ^b   Arni, Raghuvir K ^a   Fontes, Marcos R M ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b UNIVERSITY OF RIBEIRÃO PRETO   (Brazil)

Author keywords

Acidic phospholipase A 2; Bothrops jararacussu venom; Crystal structure; Dimeric phospholipase A 2; Oligomeric state; Platelet aggregation and hypotensive effects; X ray crystallography

Indexed keywords

ANTIHYPERTENSIVE AGENT; ANTITHROMBOCYTIC AGENT; CALCIUM BINDING PROTEIN; DIMER; MONOMER; OLIGOMER; PHOSPHOLIPASE A2; SNAKE VENOM; SODIUM ION; CALCIUM ION;

ACIDITY; ARTICLE; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME ISOLATION; ENZYME STRUCTURE; NUCLEOTIDE SEQUENCE; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; STRUCTURE ANALYSIS; THROMBOCYTE AGGREGATION INHIBITION; ALPHA HELIX; BINDING SITE; BOTHROPS; BOTHROPS JARARACUSSU; CONFORMATIONAL TRANSITION; PROTEIN STRUCTURE; STEREOCHEMISTRY;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BLOOD PLATELETS; BOTHROPS; CALCIUM; CHROMATOGRAPHY, ION EXCHANGE; CROTALID VENOMS; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; IONS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASES A; PLATELET AGGREGATION INHIBITORS; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM;

BOTHROPS; BOTHROPS JARARACUSSU; SERPENTES;

EID: 4444265324     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.08.046     Document Type: Article

References (49)

1. 2 enzymes: classification and characterization Biochim. Biophys. Acta 1488 2000 1 19
2. P. Rosenberg W. Shier D. Mebs Handbook of Toxinology 1990 Marcel Dekker New York 67 277
3. 2 Enzymes: Structure, Function and Mechanism 1997 Wiley Chichester 321 352
4. C.L. Ownby Structure, function and biophysical aspects of the myotoxins from snake venoms J. Toxicol. Toxin Rev. 17 1998 1003 1009
5. 2 Biochimie 82 2000 815 831
6. 2 Toxicon 27 1989 613 635
7. 2 from snake venoms: effects on catalytic and pharmacological properties. Review Toxicon 42 2004 855 868
8. P. Needleman, J. Turk, B.A. Jakschik, A.R. Morrison, and J.B. Lefkowith Arachidonic acid metabolism Annu. Rev. Biochem. 55 1986 69 102
9. 2 Arch. Biochem. Biophys. 284 1991 352 359
10. C.L. Ownby, H.S. Selistre de Araújo, S.P. White, and J.E. Fletcher Lysine 49 phospholipases A2 proteins. Review Toxicon 37 1999 411 445
11. 2 from the venom of Agkistrodon piscivorus piscivorus J. Biol. Chem. 267 1992 22645 22657
12. 2: quaternary structure and inhibition mechanism insights Biochem. Biophys. Res. Commun. 311 2003 713 720
13. 2 myotoxins from Bothrops snake venoms: structure-function relationship, Curr. Org. Chem. 8 (2004) in press
14. W.F. de Azevedo Jr., R.J. Ward, F.R. Lombardi, J.R. Giglio, A.M. Soares, M.R.M. Fontes, and R.K. Arni Crystal structure of myotoxin-II: a myotoxic phospholipase A2 homologue from Bothrops moojeni venom Protein Pept. Lett. 4 1997 329 334
15. 2 homologue Proteins: Struct. Funct. Genet. 30 1998 442 454
16. 2 - a hypothesis: the crystal structure of piratoxin II complexed to fatty acid Biochemistry 40 2001 28 36
17. R.K. Arni, M.R.M. Fontes, C. Barberato, J.M. Gutiérrez, C. Dí-az-Oreiro, and R.J. Ward Crystal structure of myotoxin II, a monomeric Lys49-phospholipase homologue isolated from the venom of Cerrophidion (Bothrops) godmani Arch. Biochem. Biophys. 366 1999 177 182
18. 2 in Bothrops snake venoms: effect of chemical modifications on the enzymatic and pharmacological properties of bothropstoxins from Bothrops jararacussu Biochimie 82 2000 755 763
19. 2 piratoxin III from Bothrops pirajai reveals unprecedented structural displacement of the calcium-binding loop: possible relationship to cooperative substrate binding Acta Crystallogr. D 59 2003 255 262
20. 2 from Bothrops neuwiedii venom Arch. Biochem. Biophys. 318 1995 65 70
21. S.M.T. Serrano, A.P. Reichl, R. Mentele, E.A. Auerswald, M.L. Santoro, C.A.M. Sampaio, A.C.M. Camargo, and M.T. Assakura A novel phospholipase A2, BJ-PLA2, from the venom of the snake Bothrops jararaca: purification, primary structure analysis, and its characterization as a platelet-aggregation- inhibiting factor Arch. Biochem. Biophys. 367 1999 26 32
22. M.I. Homsi-Brandeburgo, L.S. Queiroz, H. Santo-Neto, L. Rodrigues-Simioni, and J.R. Giglio Fractionation of Bothrops jararacussu snake venom: partial chemical characterization and biological activity of bothropstoxin Toxicon 26 1988 615 627
23. 2 activity J. Protein Chem. 17 1998 381 386
24. 2 from Bothrops jararacussu snake venom Biochem. Pharmacol. 64 2002 723 732
25. 2 from Bothrops jararacussu venom Protein J. 23 2004 273 285
26. 2 cDNA from Bothrops jararacussu venom gland Protein Expr. Purif. 37 2004 102 108
27. Z. Otwinowski, and W. Minor Processing of X-ray diffraction data collected in oscillation mode Methods Enzymol. 276 1997 307 326
28. J. Navaza AMoRe: an automated package for molecular replacement Acta Crystallogr. A50 1994 157 163
29. S.K. Han, E.T. Yoon, D.L. Scott, P.B. Sigler, and W. Cho Structural aspects of interfacial adsorption. A crystallographic and site-directed mutagenesis study of the phospholipase A2 from the venom of Agkistrodon piscivorus piscivorus J. Biol. Chem. 272 1997 3573 3582
30. A.T. Brünger, P.D. Adams, G.M. Clore, W.L. DeLano, P. Gros, R.W. Grosse-Kunstleve, J.S. Jiang, J. Kuszewski, M. Nilges, N.S. Pannu, R.J. Read, L.M. Rice, T. Simonson, and G.L. Warren Crystallography and NMR system (CNS): a new software system for macromolecular structure determination Acta Crystallogr. D54 1998 905 921
31. T.A. Jones, M. Bergdoll, and M. Kjeldgaard O: a macromolecule modeling environment C.E. Bugg S.E. Ealick Crystallographic and Modeling Methods in Molecular Design 1990 Springer-Verlag New York 189 195
32. A.T. Brünger X-PLOR Version 3.1: A System for Crystallography and NMR 1992 Yale University Press New Haven
33. Collaborative Computing Project No. 4 (CCP4), The CCP4 suite: programs for protein crystallography, Acta Crystallogr. D 50 (1994) 760-763
34. R.A. Laskowski, M.W. MacArthur, D.S. Moss, and J.M. Thornton Procheck: a program to check the stereochemical quality of protein structures J. Appl. Crystallogr. 26 1993 283 291
35. M. Carson Ribbons Methods Enzymol. 277 1997 493 505
36. 2 from the venom of Agkistrodon halys Pallas at 1.9 Å resolution Biochem. Biophys. Res. Commun. 300 2003 271 277
37. 2: relationship to interface binding Biochemistry 40 2001 609 617
38. 2 modified by p-bromo-phenacyl- bromide Toxicon 36 1998 875 886
39. L. Gu, H. Zhang, S. Song, Y. Zhou, and Z. Lin Structure of an acidic phospholipase A2 from the venom of Deinagkistrodon acutus Acta Crystallogr. D 58 2002 104 110
40. S. Brunie, J. Bolin, D. Gewirth, and P.B. Sigler The refined crystal structure of dimeric phospholipase A2 at 2.5 Å. Access to a shielded catalytic center J. Biol. Chem. 260 1985 9742 9749
41. V. Chandra, P. Kaur, J. Jasti, C. Betzel, and T.P. Singh Regulation of catalytic function by molecular association: structure of phospholipase A2 from Daboia russelli pulchella (DPLA2) at 1.9 Å resolution Acta Crystallogr. D 57 2001 1793 1798
42. K. Zhao, S. Song, Z. Lin, and Y. Zhou Structure of a basic phospholipase A2 from Agkistrodon halys Pallas at 2.13 Å resolution Acta Crystallogr. D 510 1998 510 521
43. T. Jabeen, A.K. Varma, M. Paramasivam, N. Singh, R.K. Singh, S. Sharma, A. Srinivasan, T.P. Singh, Crystal structure of a zinc containing dimer of phospholipase A2 from the venom of Indian cobra (Naja naja saggittifera), to be published
44. 2 from bovine pancreas and Crotalus atrox venom J. Biol. Chem. 260 1985 11627 11636
45. W.R. Pearson Rapid and sensitive sequence comparison with FASTP and FASTA Methods Enzymol. 183 1990 63 98
46. 2+-independent membrane damaging activity Biochemistry 40 2001 6912 6920
47. N. Dekker Outer-membrane phospholipase A: known structure, unknown biological function Mol. Microbiol. 35 2000 711 717
48. H.J. Snijder, I. Ubarretxena-Belandia, M. Blaauw, K.H. Kalk, H.M. Verheij, M.R. Egmond, N. Dekker, and B.W. Dijkstra Structural evidence for dimerization-regulated activation of an integral membrane phospholipase Nature 401 1999 717 721
49. 2 by two-photon excitation fluorescence correlation spectroscopy Biochemistry 40 2001 6903 6911