Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Song, Qing ^a   Zhang, Xiaobo ^a  

^a SOA Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources Key Laboratory of Marine Genetic   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOPHAGES; DNA; ENZYME ACTIVITY; ESCHERICHIA COLI; GENES; NUCLEIC ACIDS; PH; RNA;

NUCLEASE; NUCLEASE-ENCODING GENE; THERMOPHILIC BACTERIOPHAGE; THERMOSTABLE ENZYMES;

BIOTECHNOLOGY;

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; CITRIC ACID; DETERGENT; DITHIOTHREITOL; DODECYL SULFATE SODIUM; DOUBLE STRANDED DNA; EDETATE DISODIUM; ENZYME INHIBITOR; GUANIDINE; MERCAPTOETHANOL; NUCLEASE; NUCLEIC ACID; POLYSORBATE 20; RNA; SINGLE STRANDED DNA; TRITON X 100; UREA; ENDONUCLEASE; ENZYME ACTIVATOR; ESCHERICHIA COLI PROTEIN; EXONUCLEASE; HYBRID PROTEIN; VIRUS PROTEIN;

ARTICLE; BACTERIOPHAGE; BACTERIOPHAGE GBSV1; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE; THERMOSTABILITY; BIOSYNTHESIS; DRUG ANTAGONISM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING;

ESCHERICHIA COLI;

CAUDOVIRALES; CLONING, MOLECULAR; ENDONUCLEASES; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; EXONUCLEASES; GENE EXPRESSION; KINETICS; NUCLEIC ACIDS; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE; VIRAL REGULATORY AND ACCESSORY PROTEINS;

EID: 44249120771     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-43     Document Type: Article

References (28)

1. Mishra NC Nucleases: Molecular Biology and Applications Hoboken, NJ: John Wiley & Sons 2002.
2. Li L Lin S Yang F Functional identification of the non-specific nuclease from white spot syndrome virus Virology 2005, 337:399-406. 10.1016/ j.virol.2005.04.017 15913698
3. Maclellan SR Forsberg CW Properties of the major non-specific endonuclease from the strict anaerobe Fibrobacter succinogenes and evidence for disulfide bond formation in vivo Microbiology 2001, 147:315-323. 11158348
4. Samejima K Earnshaw WC Trashing the genome: The role of nucleases during apoptosis Nat Rev Mol Cell Biol 2005, 6:677-688. 10.1038/nrm1715 16103871
5. Ikeda S Kawasaki N Isolation and characterization of the Schizosaccharomyces pombe cDNA encoding the mitochondrial endonuclease Biochim Biophys Acta 2001, 1519:111-116. 11406279
6. Bouex P Sabourin M Chaignepain S Castroviejo M Laquel-Robert P Purification and characterization of an endo-exonuclease from Podospora anserine mitochondria Biochim Biophys Acta 2002, 1574:72-84. 11955615
7. Wu SI Lo SK Shao CP Tsai HW Hor LI Cloning and characterization of a periplasmic nuclease of Vibrio vulnificus and its role in preventing uptake of foreign DNA Appl Environ Microbiol 2001, 67:82-88. 92521 11133431 10.1128/AEM.67.1.82-88.2001
8. Sopwith WF Debrabant A Yamage M Dwyer DM Bates PA Developmentally regulated expression of a cell surface class I nuclease in Leishmania mexicana Int J Parasitol 2002, 32:449-459. 10.1016/S0020-7519(01)00372-1 11849641
9. Yamage M Debrabant A Dwyer DM Molecular characterization of hyperinducible, surface membrane-anchored, class I nuclease of trypanosomatid parasite J Biol Chem 2000, 275:36369-36379. 10.1074/ jbc.M004036200 10945983
10. Accetto T Avgustin G Non-specific DNAases from the rumen bacterium Prevotella bryantii Folia Microbiol 2001, 46:33-35. 10.1007/BF02825880
11. Al-Khaldi SF Durocher LL Martin SA Deoxyribonuclease activity in Selenomonas ruminantium, Streptococcus bovis, and Bacteriodes ovatus Curr Microbiol 2000, 41:182-186. 10.1007/s002840010115 10915204
12. Desai NA Shankae V Single-strand-specific nucleases FEMS Microbiol Rev 2003, 26:457-491. 10.1111/j.1574-6976.2003.tb00626.x 12586391
13. Rangarajan ES Shankar V Sugar non-specific endonucleases FEMS Microbiol Rev 2001, 25:583-613. 10.1111/j.1574-6976.2001.tb00593.x 11742693
14. Hsia KC Li CL Yuan HS Structural and functional insight into sugar-nonspecific nucleases in host defense Curr Opin Struct Biol 2005, 15:126-134. 10.1016/j.sbi.2005.01.015 15718143
15. Meiss G Gimadutdinow O Haberland B Pingoud A Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp. PCC 7120 endonuclease NucA and its inhibition by NuiA. J Mol Biol 2000, 297:521-534. 10.1006/ jmbi.2000.3586 10715218
16. Korn C Meiss G Gast F Gimadutdinow O Urbanke C Pingoud A Genetic engineering of Escherichia coli to produce a 1:1 complex of the Anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA Gene 2000, 253:221-229. 10.1016/S0378-1119(00)00267-5 10940560
17. Ghosh M Meiss G Pingoud A London RE Pedersen LC Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena J Biol Chem 2005, 280:27990-27997. 10.1074/jbc.M501798200 15897201
18. Vanden BB Extremophiles as a source for novel enzymes Curr Opin Microbiol 2003, 6:213-218. 10.1016/S1369-5274(03)00060-2 12831896
19. Blondal T Thorisdottir A Unnsteinsdottir U Hjorleifsdottir S Aevarsson A Ernstsson A Fridjonsson OH Skirnisdottir S Wheat JO Hermannsdottir AG Sigurdsson ST Hreggvidsson GO Smith AV Kristjansson JK Isolation and characterization of a thermostable RNA ligase 1 from a Thermus scotoductus bacteriophage TS2126 with good single-stranded DNA ligation properties Nucleic Acids Res 2005, 33:135-142. 546137 15642699 10.1093/ nar/gki149
20. Blondal T Hjorleifsdottir S Aevarsson A Fridjonsson OH Skirnisdottir S Wheat JO Hermannsdottir AG Hreggvidsson GO Smith AV Kristjansson JK Characterization of a 5′-polynucleotide kinase/3′- phosphatase from bacteriophage RM378 J Biol Chem 2005, 280:5188-5194. 10.1074/jbc.M409211200 15579472
21. Welker NE Purification and Properties of a Thermophilic Bacteriophage Lytic Enzyme J Virol 1967, 1:617-625. 375292 4990043
22. Desai NA Shankar V Purification and characterization of the single-strand-specific and guanylic-acid-preferential deoxyribonuclease activity of the extracellular nuclease from Basidiobolus haptosporus Eur J Biochem 2000, 267:5123-5135. 10.1046/j.1432-1327.2000.01580.x 10931196
23. Sui MJ Tsai LC Hsia KC Doudeva LG Chak KF Yuan HS Metal ions and phosphate binding in the H-N-H motif: Crystal structures of the nuclease domain of ColE7/Im7 in complex with a phosphate ion and different divalent metal ions Protein Sci 2002, 11:2947-2957. 10.1110/ps.0220602 12441392 2373744
24. Meiss G Franke I Gimadutdinow O Urbanke C Pingoud A Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA Eur J Biochem 1998, 251:924-934. 10.1046/ j.1432-1327.1998.2510924.x 9490069
25. Zhang W Allen S Roberts CJ Soultanas P The Bacillus subtilis Primosomal Protein DnaD Untwists Supercoiled DNA J Bacteriol 2006, 188:5487-5493. 1540042 16855238 10.1128/JB.00339-06
26. Bradford MM A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 1976, 72:248-254. 10.1016/0003-2697(76)90527-3 942051
27. Lineweaver H Burk D The determination of enzyme dissociation constants J Am Chem Soc 1934, 56:658-666. 10.1021/ja01318a036
28. Hale SP Poole LB Gerlt JA Mechanism of the reaction catalyzed by staphylococcal nuclease: Identification of the rate-determining step Biochemistry 1993, 32:7479-7487. 10.1021/bi00080a020 8338846