Purification and characterization of the single-strand-specific and guanylic-acid-preferential deoxyribonuclease activity of the extracellular nuclease from Basidiobolus haptosporus

European Journal of Biochemistry

Volumn 267, Issue 16, 2000, Pages 5123-5135

  Desai, Neelam A ^a   Shankar, Vepatu ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Base specificity; Basidiobolus haptosporus; Endoexonuclease; Extracellular nuclease; Single strand specific nuclease

Indexed keywords

ALUMINUM; AMMONIUM SULFATE; DEOXYRIBONUCLEASE; DITHIOTHREITOL; DNA; EXONUCLEASE; GUANIDINE; GUANOSINE PHOSPHATE; IRON; MERCAPTOETHANOL; MERCURY; PHOSPHATE; POTASSIUM CHLORIDE; PYROPHOSPHATE; RNA; SILVER; SODIUM CHLORIDE; ZINC;

ARTICLE; BASIDIOBOLUS HAPTOSPORUS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; PHYCOMYCETES; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; CATIONS; CHROMATOGRAPHY, DEAE-CELLULOSE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; DEOXYRIBONUCLEASES; ENDONUCLEASES; ENTOMOPHTHORALES; ENZYME STABILITY; EXONUCLEASES; FUNGAL PROTEINS; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; THERMODYNAMICS;

EID: 0033877124     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01580.x     Document Type: Article

References (51)

1. Structural specificity of five commonly used DNA nucleases
2. The nucleases of genetic recombination
3. Nuclease activities involved in DNA replication
4. Single-strand-specific nucleases
5. Buffalo (Bos bubalus) genome: Occurrence and characterization of repeated DNA sequences
6. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
7. Optimization of medium for extracellular nuclease formation from Rhizopus stolonifer
8. Purification from rat liver of a nuclease hydrolyzing ribonucleic acid and deoxyribonucleic acid
9. Extracellular ribonuclease production by Rhizopus stolonifer: Influence of metal ions
10. Studies on ribonuclease produced by Rhizopus sp. I. Crystallization and some properties of the ribonuclease
11. Active-site characterization of S1 nuclease I. Affinity purification and influence of amino-group modification
12. Microdetermination of phosphorus
13. Quantitation of protein
14. Protein measurement with the folin phenol reagent
15. Disc electrophoresis of basic proteins and peptides on polyacrylamide gels
16. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
17. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
18. Purification of a L-arabino-furanosidase using a single-column mini-scale isoelectric focusing unit
19. Colorimetric method for determination of sugars and related substances
20. Purification of proteins and peptides by SDS PAGE
21. The use of endo-β-N-acetylglucosaminidase H in characterizing the structure and function of glycoproteins
22. Purification and characterization of a nuclease (3'-nucleotidase) from a Penicillium sp
23. Isolation, characterization and primary structure of a base non-specific and adenylic acid preferential ribonuclease with higher specific activity from Trichoderma viridae
24. Single-strand-specific nucleases
25. Purification and characterization of the 2nd 5'-nucleotide-forming nuclease from Lentinus edodes
26. Purification and characterization of a nuclease from Lentinus edodes
27. Amino acid sequence of nuclease S1 from Aspergillus oryzae
28. Primary structure of nuclease P1 from Penicillium citrinum
29. Biochemical properties and hormonal regulation of barley nuclease
30. Purification and characterization of nuclease β from Ustilago maydis
31. Studies on nuclease α from Ustilago maydis
32. Purification and properties of endonuclease from wheat chloroplasts, specific for single stranded DNA
33. Partial purification and properties of an endonuclease from germinating pea seeds specific for single stranded DNA
34. Micrococcal nuclease as a probe of DNA conformation
35. A novel exocytoplasmic endonuclease from Streptomyces antibioticus
36. Purification and characterization of a nutritionally controlled endodeoxyribonuclease from Streptomyces glaucescens
37. The extracellular nuclease from Alteromonas espejiana: An enzyme highly specific for nonduplex structure in nominally duplex DNAs
38. Substrate specificity and mode of action of the zinc-metallo nuclease from Physarum polycephalum
39. A study in evolution: The DNA base sequence homology between coliphages T7 and T3
40. Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids
41. Activity of endonuclease S1 in denaturing solvents: Dimethylsulfoxide, dimethylformamide, formamide and formaldehyde
42. Specificity of the S1 nuclease from Aspergillus oryzae
43. Enzymes of nucleic acid metabolism from wheat seedlings. I. Purification and general properties of associated deoxyribonuclease, ribonuclease and 3'-nucleotidase activities
44. A comparative study of nucleases exhibiting preference for single stranded nucleic acid
45. Efficiency of T4 DNA ligase-catalyzed end joining after S1 endonuclease treatment on duplex DNA containing single stranded portions
46. Circular intermediates with missing nucleotides in the conversion of supercoiled or nicked circular to linear duplex DNA catalyzed by two species of BAL 31 nuclease
47. Mode of action of nuclease P1 on nucleic acids and its specificity for synthetic phosphodiesters
48. An endonuclease from Neurospora crassa specific for polynucleotides lacking an ordered structure. II. Studies on enzyme specificity
49. Preferential resistance of phosphodiester bonds between deoxycytidine and 5' adjacent bases to Chlamydomonas nuclease C
50. Interaction of the periplasmic dG-selective Streptomyces antibioticus nuclease with oligodeoxynucleotide substrates
51. A site-specific single strand endonuclease from eukaryote Chlamydomonas