Genetic engineering of Escherichia coli to produce a 1:1 complex of the Anabaena sp. PCC 7120 nuclease NucA and its inhibitor NuiA

Gene

Volumn 253, Issue 2, 2000, Pages 221-229

  Korn, Christian ^a   Meiss, Gregor ^a   Gast, Frank Ulrich ^a   Gimadutdinow, Oleg ^b   Urbanke, Claus ^c   Pingoud, Alfred ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b KAZAN FEDERAL UNIVERSITY   (Russian Federation)

^c HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Complex formation; Copurification; Tandem expression

Indexed keywords

ENZYME INHIBITOR; NUCLEASE;

ANABAENA; ARTICLE; CELL INCLUSION; CISTRON; ENZYME INHIBITOR COMPLEX; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION;

ALGAL PROTEINS; ANABAENA; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; ENDONUCLEASES; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; KINETICS; MACROMOLECULAR SUBSTANCES; PHOSPHODIESTERASE INHIBITORS; PHOSPHORIC DIESTER HYDROLASES; PLASMIDS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY; ULTRACENTRIFUGATION;

EID: 0034622560     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00267-5     Document Type: Article

References (28)

1. Baliaeva M.I., Kapranova M.N., Vitol M.I., Golubenko I.A., Leshchinskaia I.B. Nucleic acids utilized as the main source of bacterial nutrition. Mikrobiologiia. 45:1976;420-424.
2. Benedik M.J., Strych U. Serratia marcescens and its extracellular nuclease. FEMS Microbiol. Lett. 165:1998;1-13.
3. Dake E., Hofmann T.J., McIntire S., Hudson A., Zassenhaus H.P. Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae. J. Biol. Chem. 263:1988;7691-7702.
4. Flick K.E., Jurica M.S., Monnat R.J. Jr., Stoddard B.L. DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI. Nature. 394:1998;96-101.
5. Friedhoff P., Gimadutdinow O., Pingoud A. Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. Nucleic Acids Res. 22:1994;3280-3287.
6. Friedhoff P., Kolmes B., Gimadutdinow O., Wende W., Krause K.-L., Pingoud A. Analysis of the mechanism of the Serratia nuclease using site directed mutagenesis. Nucleic Acids Res. 24:1996;2632-2639.
7. Friedhoff P., Franke I., Krause K.-L., Pingoud A. Cleavage experiments with deoxythymidine 3′-5′(p-nitrophenyl phosphate) suggest that the homing endonuclease I-PpoI follows the same mechanism of phosphodiester bond hydrolysis as the non-specific Serratia nuclease. FEBS Lett. 443:1999;209-214.
8. Friedhoff P., Franke I., Meiss G., Wende W., Krause K., Pingoud A. A similar active site for non-specific and specific endonucleases. Nature Struct. Biol. 6:1999;112-113.
9. Garinot-Schneider C., Pommer A.J., Moore G.R., Kleanthous C., James R. Identification of putative active-site residues in the DNase domain of colicin E9 by random mutagenesis. J. Mol. Biol. 260:1996;731-742.
10. Hartley R.W. Barnase and barstar: expression of its cloned inhibitor permits expression of a cloned ribonuclease. J. Mol. Biol. 202:1988;913-915.
11. Kleanthous C., Hemmings A.M., Moore G.R., James R. Immunity proteins and their specificity for endonuclease colicins: telling right from wrong in protein-protein recognition. Mol. Microbiol. 28:1998;227-233.
12. Kolmes B., Franke I., Friedhoff P., Pingoud A. Analysis of the reaction mechanism of the non-specific endonuclease of Serratia marcescens using an artificial substrate. FEBS Lett. 397:1996;343-346.
13. Kühlmann U.C., Moore G.R., James R., Kleanthous C., Hemmings A.M. Structural parsimony in endonuclease active sites: should the number of homing endonuclease families be redefined? FEBS Lett. 463:1999;1-2.
14. Lamm, O., 1929. Arkiv för matematik, astronomi och fysik. 21B No. 2.
15. Liu X., Zou H., Slaughter C., Wang X. DFF, a heterodimeric protein that functions downstream of caspase 3 to trigger DNA fragmentation during apoptosis. Cell. 89:1997;175-184.
16. Martin G., Keller W. Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and a catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases. EMBO J. 15:1996;2593-2603.
17. Meiss G., Franke I., Gimadutdinow O., Urbanke C., Pingoud A. Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and its inhibitor NuiA. Eur. J. Biochem. 251:1998;924-934.
18. Meiss G., Gast F.U., Pingoud A. The DNA/RNA non-specific Serratia nuclease prefers double stranded A-form nucleic acids as substrates. J. Mol. Biol. 288:1999;377-390.
19. Miller M.D., Tanner J., Alpaugh M., Benedik M.J., Krause K.L. 2.1 Å structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA. Nature Struct. Biol. 1:1994;461-468.
20. Miller M.D., Cai J., Krause K.L. The active site of Serratia endonuclease contains a conserved magnesium-water cluster. J. Mol. Biol. 288:1999;975-987.
21. Muro-Pastor A.M., Flores E., Herrero A., Wolk C.P. Identification, genetic analysis and characterization of a sugar non-specific nuclease from the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Microbiol. 6:1992;3021-3030.
22. Muro-Pastor A.M., Kuritz T., Flores E., Herrero A., Wolk C.P. Transfer of a genetic marker from a megaplasmid of Anabaena sp. strain PCC 7120 to a megaplasmid of a different Anabaena strain. J. Bacteriol. 176:1994;1093-1098.
23. Muro-Pastor A.M., Herrero A., Flores E. The nuiA gene from Anabaena sp. encoding an inhibitor of the NucA sugar non-specific nuclease. J. Mol. Biol. 268:1997;589-598.
24. Pace C.N., Vajdos F., Fee L., Grimsley G., Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4:1995;2411-2425.
25. Puyet A., Greenberg B., Lacks S.A. Genetic and structural characterization of EndA: a membrane-bound nuclease required for transformation of Streptococcus pneumoniae. J. Mol. Biol. 213:1990;727-738.
26. Raaijmakers H., Vix O., Toro I., Golz S., Kemper B., Suck D. X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture. EMBO J. 18:1999;1447-1458.
27. Ruiz-Carrillo A., Cote J. Primers for mitochondrial DNA replication generated by endonuclease G. Science. 261:1993;765-769.
28. Schumann G., Cannon K., Ma W.P., Crouch R.J., Boeke J.D. Antiretroviral effect of a gag-RNaseHI fusion gene. Gene Ther. 4:1997;593-599.