Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells

Virology Journal

Volumn 5, Issue , 2008, Pages

  Ludert, Juan E ^a   Mosso, Clemente ^a   Ceballos Olvera, Ivonne ^a   Del Angel, Rosa M ^a  

^a DEPARTAMENTO DE FÍSICA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CULTURE; DENGUE VIRUS; ENZYME IMMUNOASSAY; IMMUNOLOGICAL MONITORING; MACROPHAGE; QUANTITATIVE ASSAY; VIRUS INFECTION; VIRUS REPLICATION; AEDES; ANIMAL; CELL LINE; CERCOPITHECUS; DRUG EFFECT; EVALUATION; HAMSTER; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOUSE; PHYSIOLOGY; VERO CELL; VIROLOGY; VIRUS CULTURE; VIRUS ENTRY;

DENGUE VIRUS;

AMMONIUM CHLORIDE; NS1 PROTEIN, DENGUE 1 VIRUS; NS1 PROTEIN, DENGUE-1 VIRUS; VIRUS PROTEIN;

AEDES; AMMONIUM CHLORIDE; ANIMALS; CELL LINE; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; CRICETINAE; DENGUE VIRUS; HUMANS; IMMUNOENZYME TECHNIQUES; MACROPHAGES; MICE; PLAQUE ASSAY; VERO CELLS; VIRAL NONSTRUCTURAL PROTEINS; VIRUS INTERNALIZATION; VIRUS REPLICATION;

EID: 44049104986     PISSN: None     EISSN: 1743422X     Source Type: Journal    
DOI: 10.1186/1743-422X-5-51     Document Type: Article

References (26)

1. The Pan American Health Organization. http://www.paho.org
2. Flaviviridae: the virus and their replication. BD Lindenbach CM Rice, Virology Lippincott-Raven, Philadelphia, PA, Fields BN, 2001 991 1042
3. Development of dengue virus plaques under serum-free overlay medium. B Malewicz HM Jenkin, J Clin Microbiol 1979 9 609 614 39085
4. Dengue virus plaque development in simian cell systems. I. Factors influencing virus absorption and variables in the agar overlay medium. TB Stim, Appl Microbiol 1970 19 751 756 4987200
5. Development of a fluorogenic RT-PCR system for quantitative identification of dengue virus serotypes 1-4 using conserved and serotype specific 3'noncoding sequences. HS Houng R Chung-Ming Chen DW Vaughn N Kanesa-Thasan, J Virol Meth 2001 95 19 32 10.1016/S0166-0934(01)00280-4
6. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. YY Kong CH Thai TC Tin S Davi, J Virol Meth 2006 138 123 130 10.1016/j.jviromet.2006.08.003
7. Analysis of plasma viral RNA levels during acute dengue virus infection using quantitative competitor reverse transcription-polymerase chain reaction. TM Sudiro J Zivny H Ishiko S Green DW Vaughn S Kalayanarooj A Nisalak JE Norman FA Ennis AR Rothman, J Med Virol 2001 63 29 34 10.1002/1096-9071(200101)63: 1<29::AID-JMV1004>3.0.CO;2-S 11130884
8. A simplified PCR methodology for semiquantitatively analyzing dengue viruses. S Upanam A Cabrera-Hernandez M Ekkapongpisit DR Smith, Jpn J Infect Dis 2006 59 383 387 17186958
9. Quantitative competition reverse transcription-PCR for quantification of dengue virus RNA. WK Wang CN Lee CL Kao YL Lin CC King, J Clin Microbiol 2000 38 3306 3310 10970375
10. Flow cytometry-based assay for titrating dengue virus. CR Lambeth LJ White RE Johnston AM de Silva, J Clin Microbiol 2005 43 3267 3272 16000446 10.1128/JCM.43.7.3267-3272.2005
11. Quantitation of flavivirus by fluoresce focus assay. AF Payne I Binduga-Gajewska EB Kauffman LD Kramer, J Virol Meth 2006 134 183 189 10.1016/j.jviromet.2006.01.003
12. Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization. G Winkler SE Maxwell C Ruemmler V Stoller, Virology 1989 171 302 305 10.1016/0042-6822(89)90544-8 2525840
13. Trans-complementation of yellow fever virus NS1 reveals a role in early RNA replication. BD Lindenbach CM Rice, J Virol 1997 71 9608 9617 9371625
14. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. BD Lindenbach CM Rice, J Virol 1999 73 4611 4621 10233920
15. Mutagenesis of the N-linked glycosylation sites of the yellow fever virus NS1 protein: effects on virus replication and mouse neurovirulence. IA Muylaert TJ Chambers R Galler CM Rice, Virology 1996 222 159 168 10.1006/viro.1996.0406 8806496
16. Genetic analysis of the yellow fever virus NS1 protein: identification of a temperature-sensitive mutation which blocks RNA accumulation. IR Muylaert R Galler CM Rice, J Virol 1997 71 291 298 8985349
17. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. M Flamand F Megret M Mathieu J Lepault FA Rey V Deubel, J Virol 1999 73 6104 6110 10364366
18. Enzyme-linked immunoassay specific to dengue virus type 1 non-structural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. S Alcon A Talarmin M Debruyne A Falconar V Deubel M Flamand, J Clin Microbiol 2002 40 376 381 11825945 10.1128/JCM.40.02.376-381.2002
19. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. PR Young PA Hilditch C Bletchly W Halloran, J Clin Microbiol 2000 38 1053 1057 10698995
20. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. P Dussart B Labeau G Lagathu P Louis MRT Nunes SG Rodrigues C Storck-Hermann R Cesaire J Morvan M Flamand L Baril, Clin Vacc Immunol 2006 13 1185 1189 10.1128/CVI.00229-06
21. Evaluating the sensitivity of a commercial dengue NS1-antigen capture ELISA for early diagnosis for acute dengue virus infection. V Kumarasamy SK Chua Z Hassan AHA Huahab JK Chem M Mohamad KB Chua, Singapore Med J 2007 48 669 673 17609831
22. A ligand-binding pocket in the dengue virus envelope glycoprotein. Y Modis S Ogata D Clements SC Harrison, Proc Natl Acad Sci USA 2003 100 6986 6991 12759475 10.1073/pnas.0832193100
23. Alterations of pr-M cleavage and virus export in pr-M junction chimeric dengue virus. P Keelapang R Sriburi S Supasa N Payadee A Songjaeng A Jairungsri C Puttikhung W Kasinrerk P Malasit N Sittisombut, J Virol 2004 78 2367 2381 14963133 10.1128/JVI.78.5.2367-2381.2004
24. Maturation of the dengue-2 virus NS1 protein in insect cells: effects of downstream NS2A sequences on baculovirus-expressed gene constructs. H Leblois PR Young, J Gen Virol 1995 76 979 984 9049346
25. Growth, titration and purification of alphaviruses and flaviviruses. EA Gould JC Clegg, Virology: a practical approach IRL press, Oxford, U.K, Mahy BWJ, 1991 43 78
26. Heat shock protein 90 and heat shock protein 70 are components of dengue virus receptor complex in human cells. J Reyes-Del Valle S Chavez-Salinas F Medina RM Del Angel, J Virol 2005 79 4557 4567 15795242 10.1128/JVI.79.8.4557- 4567.2005