Quantitation of flaviviruses by fluorescent focus assay

Journal of Virological Methods

Volumn 134, Issue 1-2, 2006, Pages 183-189

  Payne, Anne F ^a   Binduga Gajewska, Iwona ^a   Kauffman, Elizabeth B ^a   Kramer, Laura D ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

Dengue virus; Flavivirus; Fluorescent focus assay (FFA); Plaque assay (PA); St. Louis encephalitis virus; West Nile virus

Indexed keywords

MONOCLONAL ANTIBODY;

ANIMAL CELL; ANTIBODY LABELING; ARTICLE; CALCULATION; CELL LINE; DENGUE VIRUS; DIAGNOSTIC ACCURACY; FLAVIVIRUS; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPE; MOSQUITO; NONHUMAN; PLAQUE FORMING CELL; PRIORITY JOURNAL; SEROTYPING; ST LOUIS FLAVIVIRUS; STAINING; VERO CELL; VIRUS INFECTION; VIRUS TITRATION; WEST NILE FLAVIVIRUS;

ANIMALS; CELL LINE; CERCOPITHECUS AETHIOPS; CULICIDAE; DENGUE VIRUS; ENCEPHALITIS VIRUS, ST. LOUIS; FLAVIVIRUS; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; MICE; PLAQUE ASSAY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VERO CELLS; WEST NILE VIRUS;

DENGUE VIRUS; ST. LOUIS ENCEPHALITIS VIRUS; WEST NILE VIRUS;

EID: 33646135541     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.01.003     Document Type: Article

References (11)

1. Burke D.S., and Monath T.P. Flaviviruses. In: Knipe D.M., and Howley P.M. (Eds). Fields Virology (2001), Lippincott William & Wilkins, Philadelphia, PA 1043-1126
2. Ebel G.D., Dupuis II A.P., Ngo K.A., Nicholas D.C., Kauffman E.B., Jones S.A., Young D.M., Maffei J.G., Shi P.-Y., Bernard K.A., and Kramer L.D. Partial genetic characterization of West Nile virus strains, New York State. Emerg. Infect. Dis. 7 (2001) 650-653
3. Espinoza J.C., and Kuznar J. Rapid simultaneous detection and quantitation of infectious pancreatic necrosis virus (IPNV). J. Virol. Meth. 105 (2002) 81-85
4. Flint S.J., Enquist L.W., Racaniello V.R., and Skalka A.M. Principles of Virology: Molecular Biology, Pathogenesis, and Control of Animal Viruses. 2nd ed. (2004), ASM Press, Washington, DC
5. Igarashi A., and Mantani M. Rapid titration of dengue virus type 4 infectivity by counting fluorescent foci. Biken. J. 17 (1974) 87-93
6. Lonsdale R., Pau M.G., Oerlemans M., Ophorst C., Vooys A., Havenga M., Goudsmit J., UytdeHaag F., and Marzio G. A rapid method for immunotitration of influenza viruses using flow cytometry. J. Virol. Meth. 110 (2003) 67-71
7. Malewicz B., and Jenkin H.M. Development of dengue virus plaques under serum-free overlay medium. J. Clin. Microbiol. 9 (1979) 609-614
8. Mendez E., Ruggli N., Collett M.S., and Rice C.M. Infectious bovine viral diarrhea virus (strain NADL) RNA from stable cDNA clones: a cellular insert determines NS3 production and viral cytopathogenicity. J. Virol. 72 (1998) 4737-4745
9. Randolph V.B., and Hardy J.L. Establishment and characterization of St. Louis encephalitis virus persistent infections in Aedes and Culex mosquito cell lines. J. Gen. Virol. 69 (1988) 2189-2198
10. Stim T.B. Arborvirus plaquing in two simian kidney cell lines. J. Gen. Virol. 5 (1969) 329-338
11. Yang D.P., Goldberg K.M., Ma X.D., Magargle W., and Rappaport R. Development of a fluorescent focus identification assay using serotype-specific monoclonal antibodies for detection and quantitation of rotaviruses in a tetravalent rotavirus vaccine. Clin. Diagn. Lab. Immunol. 5 (1998) 780-783