Gene synthesis, bacterial expression and purification of the Rickettsia prowazekii ATP/ADP translocase

Biochimica et Biophysica Acta - Biomembranes

Volumn 1419, Issue 2, 1999, Pages 299-306

  Alexeyev, Mikhail F ^a   Winkler, Herbert H ^a  

^a UNIVERSITY OF SOUTH ALABAMA COLLEGE OF MEDICINE   (United States)

Author keywords

ATP; Expression; Gene synthesis; Membrane transport; Mitochondrium; Rickettsia

Indexed keywords

ADENINE NUCLEOTIDE TRANSLOCASE; ADENOSINE TRIPHOSPHATE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CODON USAGE; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE; GENE EXPRESSION; MASS SPECTROMETRY; MEMBRANE TRANSPORT; MITOCHONDRION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RICKETTSIA PROWAZEKII; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; MITOCHONDRIAL ADP, ATP TRANSLOCASES; MOLECULAR SEQUENCE DATA; RICKETTSIA PROWAZEKII; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0033062804     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2736(99)00078-4     Document Type: Article

References (31)

1. W.H. Atkinson, H.H. Winkler, Transport of AMP by Rickettsia prowazekii, J. Bacteriol. 161 (1985) 32-38.
2. W.H. Atkinson, H.H. Winkler, Permeability of Rickettsia prowazekii to NAD, J. Bacteriol. 171 (1989) 761-766.
3. H.H. Winkler, R.M. Daugherty, Acquisition of glucose by Rickettsia prowazekii through the nucleotide intermediate uridine 5′-diphosphoglucose, J. Bacteriol. 167 (1986) 805-808.
4. H.H. Winkler, Rickettsial permeability: an ADP-ATP transport system, J. Biol. Chem. 251 (1976) 389-396.
5. S.G.E. Andersson, A. Zomorodipour, J.O. Andersson, T. Sicheritz-Ponten, U.C.M. Alsmark, R.M. Podowski, A.K. Naslund, A.S. Eriksson, H.H. Winkler, C.G. Kurland, The genome sequence of Rickettsia prowazekii and the origin of mitochondria, Nature 396 (1998) 133-143.
6. E. Pfaff, M. Klingenberg, Adenine nucleotide translocation of mitochondria. 1. Specificity and control, Eur. J. Biochem. 6 (1968) 66-79.
7. G.V. Plano, H.H. Winkler, Solubilization and reconstitution of the Rickettsia prowazekii ATP/ADP translocase, J. Membr. Biol. 110 (1989) 227-233.
8. P.V. Vignais, P.M. Vignais, G. DeFaye, Adenosine diphosphate translocation in mitochondria. Nature of the receptor site for carboxyatractyloside (gummiferin), Biochemistry 12 (1973) 1508-1519.
9. P.J.F. Henderson, H.A. Lardy, E. Dorschner, Factors affecting the inhibition of adenine nucleotide translocase by bongkrekic acid, Biochemistry 9 (1970) 3453-3457.
10. P.J.F. Henderson, H.A. Lardy, Bongkrekic acid. An inhibitor of the adenine nucleotide translocase of mitochondria, J. Biol. Chem. 245 (1970) 1319-1326.
11. D.C. Krause, H.H. Winkler, D.O. Wood, Cloning and expression of the Rickettsia prowazekii ADP/ATP translocator in Escherichia coli, Proc. Natl. Acad. Sci. USA 82 (1985) 3015-3019.
12. L.R. Williamson, G.V. Plano, H.H. Winkler, D.C. Krause, D.O. Wood, Nucleotide sequence of the Rickettsia prowazekii ATP/ADP translocase-encoding gene, Gene 80 (1989) 269-278.
13. G.V. Plano, H.H. Winkler, Identification and initial topological analysis of the Rickettsia prowazekii ATP/ADP translocase, J. Bacteriol. 173 (1991) 3389-3396.
14. M.F. Alexeyev, H.H. Winkler, Membrane topology of the Rickettsia prowazekii ATP/ADP translocase revealed by novel dual pho-lac reporters, J. Mol. Biol. 285 (1999) 1503-1513.
15. R.S. Stephens, S. Kalman, C. Lammel, J. Fan, R. Marathe, L. Aravind, W. Mitchell, L. Olinger, R.L. Tatusov, Q. Zhao, E.V. Koonin, R.W. Davis, Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis, Science 282 (1998) 754-759.
16. J. Tjaden, H.H. Winkler, C. Schwoppe, M. Van Der Laan, T. Mohlmann, H.E. Neuhaus, Two nucleotide transport proteins in Chlamydia trachomatis, one for net nucleoside triphosphate uptake and the other for transport of energy, J. Bacteriol. 181 (1999) 1196-1202.
17. K. Kampfenkel, T. Möhlmann, O. Batz, M. van Montagu, D. Inzé, H.E. Neuhaus, Molecular characterization of an Arabidopsis thaliana cDNA encoding a novel putative adenylate translocator of higher plants, FEBS Lett. 374 (1995) 351-355.
18. H.E. Neuhaus, E. Thom, T. Möhlmann, M. Steup, K. Kampfenkel, Characterization of a novel ATP/ADP transporter from Arabidopsis thaliana L, Plant J. 11 (1997) 73-82.
19. T. Möhlmann, J. Tjaden, C. Schwöppe, H.H. Winkler, K. Kampfenkel, H.E. Neuhaus, Occurrence of two plastidic ATP/ADP transporters in Arabidopsis thaliana L - Molecular characterisation and comparative structural analysis of homologous ATP/ADP translocators from plastids and Rickettsia prowazekii, Eur. J. Biochem. 252 (1998) 353-359.
20. H.H. Winkler, H.E. Neuhaus, Non-mitochondrial ATP transport, Trends Biochem. Sci. 277 (1999) 64-68.
21. S.A. Dunbar, H.H. Winkler, Increased and controlled expression of the Rickettsia prowazekii ATP/ADP translocase and analysis of cysteine-less mutant translocase, Microbiology 143 (1997) 3661-3669.
22. B. Miroux, J.E. Walker, Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels, J. Mol. Biol. 260 (1996) 289-298.
23. H.H. Winkler, D.O. Wood, Codon usage in selected AT-rich bacteria, Biochimie 70 (1988) 977-986.
24. Y. Pollack, A.L. Katzen, D.T. Spira, J. Colenser, The genome of Plasmodium falciparum. I: DNA base composition, Nucleic Acids Res. 10 (1982) 539-546.
25. W. Sirawaraporn, P. Prapunwattana, R. Sirawaraporn, Y. Yuthavong, D.V. Santi, The dihydrofolate reductase domain of Plasmodium falciparum thymidylate synthase-dihydrofolate reductase, J. Biol. Chem. 268 (1993) 21637-21644.
26. C. Prodromou, L.H. Pearl, Recursive PCR: a novel technique for total gene synthesis, Protein Eng. 5 (1992) 827-829.
27. K. Jayaraman, C.J. Puccini, A PCR-mediated gene synthesis strategy involving the assembly of oligonucleotides representing only one of the strands, BioTechniques 12 (1992) 392-398.
28. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
29. P.D. Roepe, H.R. Kaback, Characterization and functional reconstitution of a soluble form of the hydrophobic membrane protein lac permease from Escherichia coli, Proc. Natl. Acad. Sci. USA 86 (1989) 6087-6091.
30. M.L. Aldema, L.M. McMurry, A.R. Walmsley, S.B. Levy, Purification of the Tn10-specified tetracycline efflux antiporter TetA in the native state as polyhistidine fusion protein, Mol. Microbiol. 19 (1996) 187-195.
31. S. Barik, Relationship between opacity of transformed E. coli colonies and over-expression of recombinant transcript, BioTechniques 22 (1997) 112-118.