Identification, effective purification and functional characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-640

Bioresource Technology

Volumn 99, Issue 9, 2008, Pages 3635-3642

  Purama, Ravi Kiran ^a   Goyal, Arun ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY GUWAHATI   (India)

Author keywords

Dextran; Glucosyltransferase; Glycoside hydrolase; Polyethylene glycol

Indexed keywords

DEXTRAN; FILTRATION; FRACTIONATION; POLYETHYLENE GLYCOLS; PURIFICATION;

GLUCOSYLTRANSFERASE; GLYCOSIDE HYDROLASE;

ENZYMES;

ANAZOLENE SODIUM; DEXTRANSUCRASE; ENZYME; FRUCTOSYLTRANSFERASE; MACROGOL; MACROGOL 1500; MACROGOL 200; MACROGOL 400; DEXTRAN; GLUCOSYLTRANSFERASE; MACROGOL DERIVATIVE; RAFFINOSE; SUCROSE;

DEXTRAN; ENZYMES; FILTRATION; FRACTIONATION; POLYETHYLENE GLYCOLS; PURIFICATION;

DYE; ELECTROKINESIS; ENZYME ACTIVITY; FILTRATION; FRACTIONATION; IDENTIFICATION METHOD; POLYMER; POLYSACCHARIDE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; FRACTIONATION; GEL FILTRATION; LEUCONOSTOC MESENTEROIDES; NONHUMAN; PERIODIC ACID SCHIFF STAIN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; ENZYME SPECIFICITY; ENZYMOLOGY; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; LEUCONOSTOC; METABOLISM;

LEUCONOSTOC MESENTEROIDES;

CHEMICAL FRACTIONATION; CHROMATOGRAPHY, GEL; DEXTRANS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUCOSYLTRANSFERASES; LEUCONOSTOC; POLYETHYLENE GLYCOLS; RAFFINOSE; SUBSTRATE SPECIFICITY; SUCROSE;

EID: 43849113287     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2007.07.044     Document Type: Article

References (25)

1. Dols M., Simeon-Remaud M., Willolt R., Vignon M., and Monson P. Characterization of the different dextransucrase activities excreted in glucose, fructose or sucrose medium by Leuconostoc mesenteroides NRRL B1299. Appl. Environ. Microbiol. 64 (1998) 298-302
2. Fukui K., Fukui Y., and Moriyama T. Purification and properties of dextransucrase and invertage from Streptococcus mutans. J. Bacteriol. 118 (1974) 796-804
3. Funane K., Yamada M., Shiraiwa M., Takahara H., Yamamoto N., Ichishima E., and Kobayashi M. Aggregated forms of dextransucrases from Leuconostoc mesenterodes NRRL B-512F and its constitutive mutant. Biosci. Biotechnol. Biochem. 59 (1995) 776-780
4. Goulas A.K., Fisher D.A., Grimble G.K., Grandison A.S., and Rastall R.A. Synthesis of isomaltoligosaccharides and oligodextrans by the combined use of dextransucrase and dextranase. Enzyme Microb. Technol 35 (2004) 327-338
5. Goyal A., and Katiyar S.S. Fractionation of Leuconostoc mesenteroides NRRL B-512F dextran sucrase by polyethylene glycol: a simple and effective method of purification. J. Microbiol. Meth. 20 (1994) 225-231
6. Holt S.M., Al-Sheikh H., and Shin K.J. Characterization of dextran-producing Leuconostoc strains. Lett. Appl. Microbiol 32 (2001) 185-189
7. Kitaoka M., and Robyt J.F. Large-scale preparation of highly purified dextransucrase from a high-producing constitutive mutant of Leuconostoc mesenteroides NRRL B-512FMC. Enzyme Microb. Technol 23 (1998) 386-391
8. Kobayashi M., and Matsuda K. Electrophoresis analysis of the multiple forms of dextransucrase from Leuconostoc mesenteroides. J. Biochem. 100 (1986) 615-621
9. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
10. Lopez-Munguia A., Pelenc V., Remaud M., Paul F., Monsan P., Biton J., Michel J.M., and Lang C. Production and purification of alternansucrase, a glucosyltransferase from Leuconostoc mesenterodes NRRL B-1355, for the synthesis of oligoalternans. Enzyme Microb. Technol 15 (1993) 77-85
11. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
12. Miekka S., and Ingham K.C. Influence of self-association of proteins on their precipitation by poly(ethylene glycol). Arch. Biochem. Biophys. 191 (1978) 525-536
13. Naessens M., Cerdobbel A., Soetaert W., and Vandamme E.J. Leuconostoc dextransucrase and dextran: production, properties and applications. J. Chem. Technol. Biotechnol. 80 (2005) 845-860
14. Nelson N. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153 (1944) 375-380
15. Nigam M., Goyal A., and Katiyar S.S. High yield purification of dextransucrase from Leuconostoc mesenteroides NRRL B-512F by phase-partitioning. J. Food. Biochem. 30 (2006) 12-20
16. Otts D.R., and Day D.F. Dextransucrase secretion in Leuconostoc mesenteroides depends on the presence of a transmembrane proton gradient. J. Bacteriol. 170 (1988) 5006-5911
17. Paul F., Auriol D., Oriol E., and Monsan P. Production and purification of dextransucrase from Leuconostoc mesenteroides NRRL B-512. Ann. NY Acad. Sci. 434 (1984) 267-270
18. Purama R.K., and Goyal A. Dextransucrase production by Leuconostoc mesenteroides. Ind. J. Microbiol. 2 (2005) 89-101
19. Quirasco M., Lopez-Munguia A., Remaud-Simeon M., Monsan P., and Farres A. Induction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F. Appl. Environ. Microbiol. 65 (1999) 5504-5509
20. Remaud-Simeon M., Paul F., Monsan P., Heyraud A., and Rinaudo M. Molecular weight characterization and structural properties of controlled molecular weight dextrans synthesized by acceptor reaction using highly purified dextransucrase. J. Carbohydr. Chem. 10 (1991) 861-876
21. Russell R.R.B. Purification of Streptococcus mutans glucosyltransferase by polyethylene glycol phase precipitation. FEMS Microbiol. Lett. 6 (1979) 197-199
22. Somogyi M. A new reagent for the determination of sugars. J. Biol. Chem. 160 (1945) 61-68
23. Tsuchiya H.M., Koepsell H.J., Corman J., Bryant G., Bogard M.O., Feger V.H., and Jackson R.W. The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides. J. Bacteriol. 64 (1952) 521-526
24. Tsuchiya H.M., Hellman N.N., Koepsell H.J., Corman J., Stringer C.S., Rogovin S.P., Bogard M.O., Bryant G., Feger V.H., Hoffman C.A., Senti F.R., and Jackson R.W. Factors affecting molecular weight of enzymatically synthesized dextran. J. Am. Chem. Soc 77 (1955) 2412-2419
25. 13 C-nuclear magnetic resonance spectroscopy of glucan extracted from natural palm wine. Food Chem 76 (2002) 287-291