Revisiting cangitoxin, a sea anemone peptide: Purification and characterization of cangitoxins II and III from the venom of Bunodosoma cangicum

Toxicon

Volumn 51, Issue 7, 2008, Pages 1303-1307

  Zaharenko, André Junqueira ^a   Ferreira Jr , Wilson Alves ^a   de Oliveira, Joacir Stolarz ^a   Konno, Katsuhiro ^b   Richardson, Michael ^c   Schiavon, Emanuele ^d   Wanke, Enzo ^d   de Freitas, José Carlos ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b Stevens Institute of Technology   (United States)

^c FUNDAÇÃO EZEQUIEL DIAS   (Brazil)

^d UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

Bunodosoma cangicum; Cangitoxin; Edman sequencing; HPLC; Patch clamp; Sea anemone

Indexed keywords

AMINO ACID; CANGITOXIN; CANGITOXIN 2; CANGITOXIN 3; PEPTIDE; PLANT TOXIN;

ANEMONE; ARTICLE; BUNODOSOMA CANGICUM; CHROMATOGRAPHY; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PURIFICATION;

AMINO ACID SEQUENCE; ANIMALS; CELLS, CULTURED; CHEMICAL FRACTIONATION; CNIDARIAN VENOMS; GANGLIA, SPINAL; HUMANS; MOLECULAR SEQUENCE DATA; NEUROTOXINS; PATCH-CLAMP TECHNIQUES; PEPTIDE FRAGMENTS; PROTEIN ISOFORMS; RATS; RATS, WISTAR; SEA ANEMONES; SODIUM CHANNELS; STRUCTURE-ACTIVITY RELATIONSHIP;

ACTINIARIA; BUNODOSOMA CANGICUM;

EID: 43849102898     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2008.01.011     Document Type: Article

References (17)

1. + current in crayfish neuromuscular system and chromaffin cells by sea anemone Bunodosoma cangicum venom. J. Neurosci. Res. 42 (1995) 539-546
2. Barhanin J., Hugues M., Schweitz H., Vincent J.P., and Lazdunski M. Structure-function relationships of sea anemone toxin II from Anemonia sulcata. J. Biol. Chem. 256 (1981) 5764-5769
3. Bosmans F., Aneiros A., and Tytgat J. The sea anemone Bunodosoma granulifera contains surprisingly efficacious and potent insect-selective toxins. FEBS Lett. 532 (2002) 131-134
4. Campos F.V., and Beirão P.S. Effects of bound ts3 on voltage dependence of sodium channel transitions to and from inactivation and energetics of its unbinding. Cell Biochem. Biophys. 44 (2006) 424-430
5. Campos F.V., Coronas F.I., and Beirão P.S. Voltage-dependent displacement of the scorpion toxin Ts3 from sodium channels and its implication on the control of inactivation. Br. J. Pharmacol. 142 (2004) 1115-1122
6. Clauser K.R., Baker P., and Burlingame A.L. Role of accurate mass measurement (±10 ppm) in protein identification strategies employing MS or MS/MS and database searching. Anal. Chem. 71 (1999) 2871-2882
7. Cunha R.B., Santana A.N., Amaral P.C., Carvalho M.D., Carvalho D.M., Cavalheiro E.A., Maigret B., Ricart C.A., Cardi B.A., Sousa M.V., and Carvalho K.M. Primary structure, behavioral and electroencephalographic effects of an epileptogenic peptide from the sea anemone Bunodosoma cangicum. Toxicon 45 (2005) 207-217
8. Honma T., and Shiomi K. Peptide toxins in sea anemones: structural and functional aspects. Mar. Biotechol. 8 (2006) 1-10
9. Lagos P., Duran R., Cervenansky C., Freitas J.C., and Silveira R. Identification of hemolytic and neuroactive fractions in the venom of the sea anemone Bunodosoma cangicum. Braz. J. Med. Biol. Res. 34 (2001) 895-902
10. Malpezzi E.L., Freitas J.C., Muramoto K., and Kamiya H. Characterization of peptides in sea anemone venom collected by a novel procedure. Toxicon 31 (1993) 853-864
11. Norton R.S. Structure and structure-function relationships of sea anemone proteins that interact with the sodium channel. Toxicon 29 (1991) 1051-1084
12. Oliveira J.S., Redaelli E., Zaharenko A.J., Cassulini R.R., Konno K., Pimenta D.C., Freitas J.C., Clare J.J., and Wanke E. Binding specificity of sea anemone toxins to Nav 1.1-1.6 sodium channels: unexpected contributions from differences in the IV/S3-S4 outer loop. J. Biol. Chem. 279 (2004) 33323-33335
13. Oliveira J.S., Zaharenko A.J., Freitas J.C., Konno K., Andrade S.A., Portaro F.C., Richardson M., Sant'anna O.A., and Tambourgi D.V. Caissarolysin I (Bcs I), a new hemolytic toxin from the Brazilian sea anemone Bunodosoma caissarum: purification and biological characterization. Biochim. Biophys. Acta 1760 (2006) 453-461
14. + channel toxin BcIII. Biochim. Biophys. Acta 1764 (2006) 1592-1600
15. Salceda E., Garateix A., and Soto E. The sea anemone toxins BgII and BgIII prolong the inactivation time course of the tetrodotoxin-sensitive sodium current in rat dorsal root ganglion neurons. J. Pharmacol. Exp. Ther. 303 (2002) 1067-1074
16. Santana A.N., Leite A.B., Franca M.S., Franca L., Vale O.C., Cunha R.B., Ricart C.A., Sousa M.V., and Carvalho K.M. Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera. Braz. J. Med. Biol. Res. 31 (1998) 1335-1338
17. Schweitz H., Bidard J.N., Frelin C., Pauron D., Vijverberg H.P., Mahasneh D.M., Lazdunski M., Vilbois F., and Tsugita A. Purification, sequence, and pharmacological properties of sea anemone toxins from Radianthus paumotensis. A new class of sea anemone toxins acting on the sodium channel. Biochemistry 24 (1985) 3554-3561