The toxic effect of pyrrolizidine alkaloid clivorine on the human embryonic kidney 293 cells and its primary mechanism

Experimental and Toxicologic Pathology

Volumn 60, Issue 1, 2008, Pages 87-93

  Ji, Li Li ^a,b   Chen, Ying ^a,c   Wang, Zheng Tao ^a,b,c  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b Shanghai R and D Centre for Standardization of Chinese Medicines   (China)

^c CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Clivorine; Cytotoxicity; HEK293 cell; JNK; MAPK; Pyrrolizidine alkaloid

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; ANTHRA[1,9 CD]PYRAZOL 6(2H) ONE; CASPASE 3; CASPASE 9; CLIVORINE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE P38; STRESS ACTIVATED PROTEIN KINASE;

AGAR GEL ELECTROPHORESIS; APOPTOSIS; ARTICLE; CELL DEATH; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STRAIN HEK293; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DNA FRAGMENTATION; HUMAN; HUMAN CELL; PROTEIN PHOSPHORYLATION;

ANTHRACENES; APOPTOSIS; ASTERACEAE; CASPASE 3; CASPASE 9; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUGS, CHINESE HERBAL; ENZYME INHIBITORS; HUMANS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; KIDNEY; MITOGEN-ACTIVATED PROTEIN KINASE 3; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PYRROLIZIDINE ALKALOIDS;

LIGULARIA HODGSONII;

EID: 43849093107     PISSN: 09402993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.etp.2007.11.010     Document Type: Article

References (23)

1. Bonni A., Brunet A., West A.E., Datta S.R., Takasu M.A., and Greenberg M.E. Cell survival promoted by the Ras-MAPK signaling pathway by the transcription-dependent and -independent mechanisms. Science 286 (1999) 1358-1361
2. Boyce M., Degterev A., and Yuan J. Caspases: an ancient cellular sword of Damocles. Cell Death Differ 11 (2004) 29-37
3. Chang L., and Karin M. Mammalian MAP kinase signalling cascades. Nature 410 (2001) 37-40
4. Coulombe R.A. Pyrrolizidine alkaloids in foods. In: Taylor S.L. (Ed). Advances in food and nutrition research vol. 45 (2003), Elsevier Science, Oxford 61-99
5. Cui Y., and Lin G. Simultaneous analysis of clivorine and its four microsomal metabolites by high-performance liquid chromatography. J Chromatogr A 903 (2000) 85-92
6. De Maria R., Lenti L., Malisan F., d'Agostino F., Tomassini B., Zeuner A., et al. Requirement of GD3 ganglioside in CD95- and ceraminde-induced apoptosis. Science 277 (1997) 1652-1655
7. Fraser A., and Evan G. A license to kill. Cell 85 (1996) 781-784
8. Hansen M.B., Nielsen S.E., and Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 119 (1989) 203-210
9. Ji L.L., Zhao X.G., Chen L., Zhang M., and Wang Z.T. Pyrrolizidine alkaloid clivorine inhibits human normal liver L-02 cells growth and activates p38 mitogen-activated protein kinase in L-02 cells. Toxicon 40 (2002) 1685-1690
10. Ji L.L., Zhang M., Sheng Y.C., and Wang Z.T. Pyrrolizidine alkaloid clivorine induces apoptosis in human normal liver L-02 cells and reduces the expression of p53 protein. Toxicol In Vitro 19 (2005) 41-46
11. Kuhara K., Takanashi H., Hirono I., Furuya T., and Asada Y. Carcinogenic activity of clivorine, a pyrrolizidine alkaloid isolated from Ligularia Dentata. Cancer Lett 10 (1980) 117-122
12. Lappin P.B., Ross K.L., King L.E., Fraker P.J., and Roth R.A. The response of pulmonary vascular endothelial cells to monocrotaline pyrroles: cell proliferation and DNA synthesis in vitro and in vivo. Toxicol Appl Pharmacol 150 (1998) 37-48
13. Lewis T.S., Shapiro P.S., and Ahn N.G. Signal transduction through MAP kinase cascades. Adv Cancer Res 74 (1998) 49-139
14. Lin G., Cui Y.Y., and Hawes E.M. Microsomal formation of a pyrrolic alcohol glutathione conjugate of clivorine. Firm evidence for the formation of a pyrrolic metabolite of an otonecine-type pyrrolizidine alkaloid. Drug Metab Dispos 26 (1998) 181-184
15. Lin G., Cui Y.Y., and Hawes E.M. Characterization of rat liver microsomal metabolites of clivorine, an hepatotoxic otonecine-type pyrrolizidine alkaloid. Drug Metab Dispos 28 (2000) 1475-1483
16. Nishina H., Wada T., and Katada T. Physiological roles of SAPK/JNK signaling pathway. J Biochem (Tokyo) 136 (2004) 123-126
17. Nyska A., Moomaw C.R., Foley J.F., Maronpot R.R., Malarkey D.E., Cummings C.A., et al. The hepatic endothelial carcinogen riddelliine induces endothelial apoptosis, mitosis, S phase, and p53 and hepatocytic vascular endothelial growth factor expression after short-term exposure. Toxicol Appl Pharmacol 184 (2002) 153-164
18. Petty R.D., Sutherland L.A., Hunter E.M., and Cree I.A. Comparison of MTT and ATP-based assays for the measurement of viable cell number. J Biolumin Chemilumin 10 (1995) 29-34
19. Wilson D.W., Lame M.W., Dunston S.K., and Segall H.J. DNA damage cell checkpoint activities are altered in monocrotaline pyrrole-induced cell cycle arrest in human pulmonary artery endothelial cells. Toxicol Appl Pharmacol 166 (2000) 69-80
20. Won S.M., Park Y.H., Kim H.J., Park K.M., and Lee W.J. Catechins inhibit angiotensin II-induced vascular smooth muscle cell proliferation via mitogen-activated protein kinase pathway. Exp Mol Med 38 (2006) 525-534
21. Xia Q., Chou M.W., Lin G., and Fu P.P. Metabolic formation of DHP-derived DNA adducts from a representative otonecine type pyrrolizidine alkaloid clivorine and the extract of Ligularia hodgsonnii Hook. Chem Res Toxicol 17 (2004) 702-708
22. Yamanaka H., Nagao M., Sugimura T., Furuya T., Shirai A., and Matsushima T. Mutagenicity of pyrrolizidine alkaloids in the Salmonella/mammalian-microsome test. Mutat Res 68 (1979) 211-216
23. Zhang Y.G., Mattjus P., Schmid P.C., Dong Z.M., Zhong S.P., Ma W.Y., et al. Involvement of the acid sphingomyelinase pathway in UVA-induced apoptosis. J Biol Chem 276 (2001) 11775-11782