Secretion of active urokinase-type plasminogen activator from the yeast Yarrowia lipolytica

Biotechnology and Bioprocess Engineering

Volumn 8, Issue 2, 2003, Pages 162-165

  Ryu, Ho Myoung ^a   Kang, Woo Kyu ^a   Kang, Hyun Ah ^b   Kim, Jeong Yoon ^a  

^a Chungnam National University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Secretion; Urokinase type plasminogen activator; Yarrowia lipolytica

Indexed keywords

EID: 43749090351     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02940274     Document Type: Article

References (21)

1. Faber, K. N., W. Harder, G. Ab, and M. Veenhuis (1995) Review: methylotrophic yeasts as factories for the production of foreign proteins. Yeast 11: 1331-1344.
2. Ngsee, J. K. and M. Smith (1990) Changes in a mammalian signal sequence required for efficient protein secretion by yeasts. Gene 86: 251-255.
3. Schwientek, T. and J. F. Ernst (1994) Efficient intra- and extracellular production of human beta-l,4-galactosyltransferase in Saccharomyces cerevisiae is mediated by yeast secretion leaders. Gene 145: 299-303.
4. Barth, G. and C. Gaillardin (1997) Physiology and genetics of the dimorphic fungus Yarrowia lipolytica. FEMS Microbiol. Rev. 19:219-237.
5. Juretzek, T., M. Le Dall, S. Mauersberger, C. Gaillardin, G. Barth, and J. Nicaud (2001) Vectors for gene expression and amplification in the yeast Yarrowia lipolytica. Yeast 18: 97-113.
6. Park, C. S., C. C. Chang, J. Y. Kim, D. M. Ogrydziak, and D. D. Ryu (1997) Expression, secretion, and processing of rice alpha-amylase in the yeast Yarrowia lipolytica. J. Biol. Chem. 272:6876-6881.
7. Pignede, G., H. Wang, F. Fudalej, C. Gaillardin, M. Seman, and J. M. Nicaud (2000) Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolytica. J. Bacteriol. 182: 2802-2810.
8. Chang, C. C., D. D. Ryu, C. S. Park, and J.-Y. Kim (1998) Improvement of heterologous protein productivity using recombinant Yarrowia lipolytica and cyclic fed-batch process strategy. Biotechnol. Bioeng. 59: 379-385.
9. 2+-ATPase, in the yeast Yarrowia lipolytica. Gene 206: 107-116.
10. Kim, J. W., T. J. Park, D. D. Ryu, and J.-Y. Kim (2000) High cell density culture of Yarrowia lipolytica using a one-step feeding process. Biotechnol. Prog. 16: 657-660.
11. Pagot, Y., A. Endrizzi, J. M. Nicaud, and J.M. Belin (1997) Utilization of an auxotrophic strain of the yeast Yarrowia lipolytica to improve gamma-decalactone production yields. Lett. Appl. Microbiol. 25: 113-116.
12. Pagot, Y., A. Le Clainche, J. M. Nicaud, Y. Wache, and J. M. Belin (1998) Peroxisomal beta-oxidation activities and gamma-decalactone production by the yeast Yarrowia lipolytica. Appl. Microbiol. Biotechnol. 49: 295-300.
13. Lijnen, H. R. and D. Collen (1991) Strategies for the improvement of thrombolytic agents. Thromb. Haemost. 66: 88-110.
14. Wun, T. C., L. Ossowski, and E. Reich (1982) A proenzyme form of human urokinase. J. Biol. Chem. 257: 7262-7268.
15. Holmes, W. E., D. Pennica, M. Blaber, M. W. Rey, W. A. Guenzler, G. J. Steffens, and H. L. Heyneker (1985) Cloning and expression of the gene for pro-urokinase in Escherichia coli. Bio/Technology 3: 923-929.
16. Melnick, L. M., B. G. Turner, P. Puma, B. Price-Tillotson, K. A. Salvato, D. R. Dumais, D. T. Moir, R. J. Broeze, and G. C. Avgerinos (1990) Characterization of a nonglycosylated single chain urinary plasminogen activator secreted from yeast. J. Biol. Chem. 265: 801-807.
17. Hiramatsu, R., S. Horinouchi, and T. Beppu (1991) Isolation and characterization of human pro-urokinase and its mutants accumulated within the yeast secretory pathway. Gene 99: 235-241.
18. Tsujikawa, M., K. Okabayashi, M. Morita, and T. Tanabe (1996) Secretion of a variant of human single-chain urokinase-type plasminogen activator without an N-glycosylation site in the methylotrophic yeast, Pichia pastoris and characterization of the secreted product. Yeast 12: 541-553.
19. Agaphonov, M. O., A. N. Packeiser, M. B. Chechenova, E. S. Choi, and M. D. Ter-Avanesyan (2001) Mutation of the homologue of GDP-mannose pyrophosphorylase alters cell wall structure, protein glycosylation and secretion in Hansenula polymorpha. Yeast 18: 391-402.
20. Nelles, L., H. R. Lijnen, D. Collen, and W. E. Holmes (1987) Characterization of recombinant human single chain urokinase-type plasminogen activator mutants produced by site-specific mutagenesis of lysine 158. J. Biol. Chem. 262: 5682-5689.
21. Ogrydziak, D. M. and R. K. Mortimer (1977) Genetics of extracellular protease production in Saccharomycopsis lipolytica. Genetics 87: 621-632.