Enzymatic detection of heavy metal ions in aqueous solution from vegetable wastes by immobilizing pumpkin (Cucumis melo) urease in calcium alginate beads

Biotechnology and Bioprocess Engineering

Volumn 13, Issue 2, 2008, Pages 210-216

  Prakash, Om ^a   Talat, Mahe ^a   Hasan, S H ^b   Pandey, Rajesh K ^a  

^a BANARAS HINDU UNIVERSITY   (India)

^b BANARAS HINDU UNIVERSITY   (India)

Author keywords

Calcium alginate; Cucumis melo; Immobilization; Inhibition; Pumpkin; Urease

Indexed keywords

ACETIC ACID; CADMIUM; CALCIUM ALGINATE; COBALT; COPPER; HEAVY METAL; MERCURY; SEPHADEX; UREASE;

AQUEOUS SOLUTION; ARTICLE; CANTALOUPE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME ISOLATION; ENZYME PURIFICATION; GEL FILTRATION; HEAT TREATMENT; HEAVY METAL POISONING; IMMOBILIZATION; INCUBATION TIME; LEACHING; PLANT SEED; PUMPKIN; QUANTITATIVE ANALYSIS; VEGETABLE; WATER POLLUTION;

CUCUMIS MELO;

EID: 43749088733     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-007-0179-5     Document Type: Article

References (24)

1. Ghindilis, A. L., P. Atanasov, and E. Wilkins (1997) Enzyme catalyzed direct electron transfer: fundamentals and analytical applications. Electroanalysis 9: 661-674.
2. Jung, K., G. Bitton, and B. Koopman (1995) Assessment of urease inhibition assays for measuring toxicity of environmental samples. Water Res. 29: 1929-1933.
3. Duran, N., M. A. Rosa, A. D'Annibale, and L. Gianfreda (2002) Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review. Enzyme Microb. Technol. 31: 907-931.
4. Kuswandi, B. (2003) Simple optical fibre biosensor based on immobilized enzyme for monitoring of trace heavy metal ions. Anal. Bioanal. Chem. 376: 1104-1110.
5. Prakash, O. and D. K. Vishwakarma (2001) Inhibition of urease from seeds of water melon (Citrullus vulgaris) by heavy metal ions. J. Plant Biochem. Biotechnol. 10: 147-149.
6. Vel Krawczyk, T. K., M. Moszczynska, and M. Trojanowicz (2000) Inhibitive determination of mercury and other metal ions by potentiometric urea biosensor. Biosens. Bioelectron. 15: 681-691.
7. Srivastava, P. K., A. M. Kayastha, and Srinivasan (2001) Characterization of gelatin-immobilized pigeonpea urease and preparation of a new urea biosensor. Biotechnol. Appl. Biochem. 34: 55-62.
8. Prakash, O., S. Puliga, and L. S. B. Upadhyay (2007) Immobilization of watermelon (Citrullus vulgaris) urease in agarose gel for urea estimation. Biotechnol. Bio-process Eng. 12: 131-135.
9. Kayastha, A. M. and P. K. Srivastava (2001) Pigeonpea (Cajanus cajan L.) urease immobilized on glutaraldehyde-activated chitosan beads and its analytical applications. Appl. Biochem. Biotechnol. 96: 41-53.
10. Reddy K, R. C., P. K. Srivastava, P. M. Dey, and A. M. Kayastha (2004) Immobilization of pigeonpea (Cajanus cajan) urease on DEAE-cellulose paper strips for urea estimation. Biotechnol. Appl. Biochem. 39: 323-327.
11. Smidsrod, O. and G. Skjak-Braek (1990) Alginate as immobilization matrix for cells. Trends Biotechnol. 8: 71-78.
12. Kierstan, M. and C. Bucke (2000) The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels. Biotechnol. Bioeng. 67: 726-736.
13. 2+ ion inhibition of jack bean urease. J. Inorg. Biochem. 98: 1160-1168.
14. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
15. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
16. Blattler, D. P., C. C. Contaxis, and F. J. Reithel (1967) Dissociation of urease by glycol and glycerol. Nature 216: 274-275.
17. Lee, S. M. and W. Y. Lee (2002) Determination of heavy metal ions using conductometric biosensor based on sol-gel-immobilized urease. Bull. Kor. Chem. Soc. 23: 1169-1172.
18. Rodriguez, B. B., J. A. Bolbot, and I. E. Tothill (2004) Development of urease and glutamic dehydrogenase amperometric assay for heavy metals screening in polluted samples. Biosens. Bioelectron. 19: 1157-1167.
19. Fahmy, A. S., V. B. Bagos, and T. M. Mohammed (1998) Immobilization of Citrullus vulgaris urease on cyanuric chloride DEAE-cellulose ether: preparation and properties. Bioresour. Technol. 64: 121-129.
20. Krajewska, B. (1991) Urease immobilized on chitosan membrane. Inactivation by heavy metal ions. J. Chem. Technol. Biotechnol. 52: 157-162.
21. Srivastava, P. K. and A. M. Kayastha (2000) Significance of sulfhydryl groups in the activity of urease from pigeonpea (Cajanus cajan L.) seeds. Plant Sci. 159: 149-158.
22. Prakash, O. and L. S. B. Upadhyay (2003) Effect of thiols on the activity of urease from dehusked seeds of watermelon (Citrullus vulgaris). Plant Sci. 164: 189-194.
23. Prakash, O. and G. Bhushan (1998) Molecular asymmetry in urease of watermelon (Citrullus vulgaris). J. Plant Biochem. Biotechnol. 7: 53-55.
24. Prakash, O. and L. S. B. Upadhyay (2006) Immobilization imparts stability to watermelon urease to work in water miscible organic media. Biotechnol. Bioprocess Eng. 11: 140-145.