Immobilization imparts stability to watermelon urease to work in water miscible organic media

Biotechnology and Bioprocess Engineering

Volumn 11, Issue 2, 2006, Pages 140-145

  Prakash, Om ^a   Upadhyay, Lata Sheo Bachan ^a  

^a BANARAS HINDU UNIVERSITY   (India)

Author keywords

Citrullus vulgaris; Immobilized urease; Urease; Water miscible solvents; Watermelon

Indexed keywords

ACETONITRILE; ALCOHOL; ALGINIC ACID; CALCIUM ALGINATE; METHANOL; N,N DIMETHYLFORMAMIDE; ORGANIC SOLVENT; PROPANOL; UREASE; WATER;

ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STABILITY; ENZYME SUBSTRATE; PROTEIN IMMOBILIZATION; SOLUBILITY; TEMPERATURE MEASUREMENT; TIME; WATERMELON;

CITRULLUS LANATUS VAR. LANATUS;

EID: 33646429780     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931898     Document Type: Article

References (26)

1. Zaks, A. and A. M. Klibanov (1984) Enzymatic catalysis in organic media at 100°C. Science 224: 1249-1251.
2. Griebenow, K. and A. M. Klibanov (1996) On protein denaturation in aqueous-organic mixtures but not in pure organic solvents. J. Am. Chem. Soc. 118: 11695-11700.
3. Schmitke, J. L., L. J. Stern, and A. M. Klibanov (1997) The crystal structure of subtilisin Carlsberg in anhydrous dioxane and its comparison with those in water and acetonitrile. Proc. Natl. Acad. Sci. USA 94: 4250-4255.
4. Zhu, G., Q. Huang, Z. Wang, M. Qian, Y. Jia, and Y. Tang (1998) X-ray studies on two forms of bovine β-trypsin crystals in neat cyclohexane. Biochim. Biophys. Acta 1429: 142-150.
5. Zaks, A. and D. R. Dodds (1997) Application of biocatalysis and biotransformations to the synthesis of pharmaceuticals. Drug Discov. Today 2: 513-531.
6. Luisi, P. L. and C. Laane (1986) Solubilization of enzymes in apolar solvents via reverse micelles. Trends Biotechnol. 4: 153-161.
7. Klibanov, A. M. (1989) Enzymatic catalysis in anhydrous organic solvents. Trends Biochem. Sci. 14: 141-144.
8. Zaks, A. and A. M. Klibanov (1988) Enzymatic catalysis in nonaqueous solvents. J. Biol. Chem. 263: 3194-3201.
9. Carrea, G., G. Ottolina, and S. Riva (1995) Role of solvents in the control of enzyme selectivity in organic media. Trends Biotechnol. 13: 63-70.
10. Okahata, Y., M. Yamaguchi, F. Tanaka, and I. Fujii (1995) A lipid-coated catalytic antibody in water-miscible organic solvents. Tetrahedron 51: 7673-7680.
11. Tanaka, K. and Y. Okahata (1996) A DNA-lipid complex in organic media and formation of an aligned cast film. J. Am. Chem. Soc. 118: 10679-10683.
12. Carrea, G. and S. Riva (2000) Properties and synthetic applications of enzymes in organic solvents. Angew. Chem. Int. Ed. Engl. 39: 2226-2254.
13. Prakash, O. and G. Bhushan (1997) Isolation, purification, and partial characterization of urease from seeds of water melon (Citrullus vulgaris). J. Plant Biochem. Biotechnol. 6: 45-47.
14. Prakash, O. and G. Bhushan (1998) A study of inhibition of urease from seeds of the water melon (Citrullus vulgaris). J. Enzym. Inhib. 13: 69-77.
15. Prakash, O. and G. Bhushan (1998) Molecular asymmetry in urease of water melon (Citrullus vulgaris). J. Plant Biochem. Biotechnol. 7: 53-55.
16. Blakeley, R. L. and B. Zerner (1984) Jack bean urease: the first nickel enzyme. J. Mol. Catal. 23: 263-292.
17. Prakash, O. and L. S. B. Upadhyay (2003) Effect of thiols on the activity of urease from dehusked seeds of watermelon (Citrullus vulgaris). Plant Sci. 164: 189-194.
18. Prakash, O. and L. S. B. Upadhyay (2005) Physicochemical characterization of watermelon urease upon immobilization in alginate beads. J. Plant Biochem. Biotechnol. 14: 209-213.
19. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
20. Janda, K. D., J. A. Ashley, T. M. Jones, D. A. McLeod, D. M. Schloeder, and M. I. Weinhouse (1990) Immobilized catalytic antibodies in aqueous and organic solvents. J. Am. Chem. Soc. 112: 8886-8888.
21. Anupma (1997) Immobilization and Chemical Modification of Proteases. Ph.D. Thesis. Banaras Hindu University, Varanasi, India.
22. Adlercreutz, F. (1991) On the importance of the support material for enzymatic synthesis in organic media. Support effects at controlled water activity. Eur. J. Biochem. 199: 609-614.
23. Khmelnitsky, Y. L., S. H. Welch, D. S. Clark, and J. S. Dordick (1994) Salts dramatically enhance activity of enzymes suspended in organic solvents. J. Am. Chem. Soc. 116: 2647-2648.
24. Clark, D. S. and J. E. Bailey (1983) Structure-function relationships in immobilized chymotrypsin catalysis. Biotechnol. Bioeng. 25: 1027-1047.
25. Clark, D. S. and J. E. Bailey (1984) Characterization of heterogeneous immobilized enzyme subpopulations using EPR spectroscopy. Biotechnol. Bioeng. 26: 231-238.
26. Skerker, P. S. and D. S. Clark (1988) Catalytic properties and active-site structural features of immobilized horse liver alcohol dehydrogenase. Biotechnol. Bioeng. 32: 148-158.