Site-directed mutagenesis of firefly luciferase: Implication of conserved residue(s) in bioluminescence emission spectra among firefly luciferases

Biochemical Journal

Volumn 412, Issue 1, 2008, Pages 27-33

  Tafreshi, Narges Kh ^a   Sadeghizadeh, Majid ^a   Emamzadeh, Rahman ^a   Ranjbar, Bijan ^a   Naderi Manesh, Hossein ^a   Hosseinkhani, Saman ^a  

^a TARBIAT MODARES UNIVERSITY   (Iran)

Author keywords

Bioluminescence; Firefly luciferase; Lampyris turkestanicus (Iranian firefly); Mutagenesis; Red shift

Indexed keywords

BIOASSAY; BIOLUMINESCENCE; MUTAGENESIS; TISSUE;

BIOLUMINESCENCE IMAGING; LUCIFERASE REACTION; RED-EMITTING LUCIFERASES;

ENZYME ACTIVITY;

FIREFLY LUCIFERASE;

ADENYLATION; ARTICLE; BIOLUMINESCENCE; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME STRUCTURE; FIREFLY; FLAME PHOTOMETRY; GENE MUTATION; LAMPYRIS TURKESTANICUS; NONHUMAN; OXIDATION; PHRIXOTHRIX; PRIORITY JOURNAL; PROTEIN EXPRESSION; RED LIGHT; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; WORM;

ANIMALS; CONSERVED SEQUENCE; FIREFLIES; GENES, REPORTER; KINETICS; LUCIFERASES, FIREFLY; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN DENATURATION; SEQUENCE HOMOLOGY, AMINO ACID; SPECTRUM ANALYSIS;

COLEOPTERA; LAMPYRIS TURKESTANICUS; PHENGODIDAE; PHRIXOTHRIX;

EID: 43549103310     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070733     Document Type: Article

References (43)

1. White, E. H., Rapaport, E., Seliger, H. H. and Hopkins, T. A. (1971) The chemi- and bioluminescence of firefly luciferin: an efficient chemical production of electronically excited states. Bioorg. Chem. 1, 92-122
2. DeLuca, M. (1976) Firefly luciferase. Adv. Enzymol. Relat. Areas Mol. Biol. 44, 37-68
3. Doyle, T. C., Burns, S. M. and Contag, C. H. (2004) In vivo bioluminescence imaging for integrated studies of infection. Cell. Microbiol. 6, 303-317
4. Roda, A., Pasini, P., Mirasoli, M., Michelini, E. and Guardigli, M. (2004) Biotechnological application of bioluminescence and chemiluminescence. Trends Biotechnol. 22, 295-303
5. Branchini, B. R., Southworth, T. R., Khattak, N. F., Michelini, E. and Roda, A. (2005) Red and green emitting firefly luciferase mutants for bioluminescent reporter application. Anal. Biochem. 345, 140-148
6. Billard, P. and DuBow, M. S. (1998) Bioluminescence-based assay for detection and characterization of bacteria and chemicals in clinical laboratories. Clin. Biochem. 31, 1-14
7. Bitton, G. and Koopman, B. (1992) Bacterial and enzymatic bioassays for toxicity testing in the environment. Rev. Environ. Contam. Toxicol. 125, 1-22
8. Cheong, W. F., Prahl, S. A. and Welch, A. J. (1990) A review of the optical properties of biological tissues. IEEE J. Quantum Electron. 26, 2166-2185
9. Rice, B. W., Cable, M. D. and Nelson, M. B. (2001) In vivo imaging of light-emitting probes. J. Biomed. Opt. 6, 432-440
10. Tromberg, B. J., Shah, N., Lanning, R., Cerussi, A., Espinoza, J., Pham, T., Svaasand, L. and Butler, J. (2000) Non-invasive in vivo characterization of breast tumors using photon migration spectroscopy. Neoplasia 2, 26-40
11. Shapiro, E., Lu, C. and Baneyx, F. (2005) A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications. Protein Eng. Des. Sel. 18, 581-587
12. Branchini, B. R., Magyar, R. A., Murtiashaw, M. H., Anderson, S. M. and Zimmer, M. (1998) Site-directed mutagenesis of histidine 245 in firefly luciferase: a proposed model of the active site. Biochemistry 37, 15311-15319
13. Branchini, B. R., Magyar, R. A., Murtiashaw, M. H. and Portier, N. C. (2001) The role of active site residue arginine 218 in firefly luciferase bioluminescence. Biochemistry 40, 2410-2418
14. Branchini, B. R., Southworth, T. L., Murtiashaw, M. H., Boije, H. and Fleet, S. E. (2003) A mutagenesis study of the putative luciferin binding site residues of firefly luciferase. Biochemistry 42, 10429-10436
15. Kajiyama, N. and Nakano, E. (1991) Isolation and characterization of mutants of firefly luciferase which produce different colors of light. Protein Eng. 4, 691-693
16. Wood, K. V., Lam, A. Y., Seliger, H. H. and McElroy, W. D. (1989) Complementary DNA coding click beetle luciferases can elicit bioluminescence of different colors. Science 244, 700-702
17. Wood, K. V. (1995) The chemical mechanism and evolutionary development of beetle bioluminescence. Photochem. Photobiol. 62, 662-673
18. Ohmiya, Y., Hirano, T. and Ohashi, M. (1996) The structural origin of the color differences in the bioluminescence of firefly luciferase. FEBS Lett. 384, 83-86
19. Viviani, V. R., Neto, A. J. S. and Ohmiya, Y. (2004) The influence of the region between residues 220 and 344 and beyond in Phrixotrix railroad worm luciferases green and red bioluminescence. Protein Eng. 17, 113-117
20. Ugarova, N. N. and Brovko, L. Y. (2001) Relationship between the structure of the protein globule and bioluminescence spectra of firefly luciferase. Russ. Chem. Bull. 50, 1752-1761
21. DeLuca, M. (1969) Hydrophobic nature of the active site of firefly luciferase. Biochemistry 8, 160-166
22. White, E. H. and Branchini, B. (1975) Modification of firefly luciferase with a luciferin analog: a red light producing enzyme. J. Am. Chem. Soc. 97, 1243-1245
23. Viviani, V. R. and Bechara, E. J. H. (1995) Bioluminescence of Brazilian fireflies (Coleoptera: Lampyridae): spectral distribution and pH effect on luciferase-elicited colors. Comparison with elaterid and phengodid luciferases. Photochem. Photobiol. 62, 490-495
24. McCapra, F., Gilfoyle, D. J., Young, D. W., Church, N. J. and Spencer, P. (1994) The chemical origin of colour differences in beetle bioluminescence. In Bioluminescence and Chemiluminescence: Fundamentals and Applied Aspects (Campbell, A. K., Kricka, L. J. and Stanley, P. E., eds), pp. 387-391, Wiley, Chichester
25. McCapra, F. (1996) Mechanism in chemiluminescence and bioluminesce: unfinished business. In Bioluminescence and Chemiluminescence: Molecular Reporting with Photons (Hasting, J. W., Kricka, L. J. and Stanley, P. E., eds), pp. 7-15, Wiley, Chichester
26. Branchini, B. R., Southworth, T. L., Murtiashaw, M. H., Magyar, R. A., Gonzalez, S. A., Ruggiero, M. C. and Stroh, J. G. (2004) An alternative mechanism of bioluminescence color determination in firefly luciferase. Biochemistry 43, 7255-7262
27. Conti, E., Franks, N. P. and Brick, P. (1996) Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes. Structure 4, 287-298
28. Nakatsu, T., Ichiyama, S., Hiratake, J., Saldanha, A., Kobashi, N., Sakata, K. and Kato, H. (2006) Structural basis for the spectral difference in luciferase bioluminescence. Nature 440, 372-376
29. Alipour, B. S., Hosseinkhani, S., Nikkhah, M., Naderi-Manesh, H., Chaichi, M. J. and Kazempour, S. (2004) Molecular cloning, sequence analysis, and expression of a cDNA encoding the luciferase from the glow-worm Lampyris turkestanicus. Biochem. Biophys. Res. Commun. 325, 215-222
30. Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K. and Pease, L. R. (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77, 61-68
31. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
32. Schippers, P. H. and Dekkers, H. P. J. M. (1981) Direct determination of absolute circular dichroism data and calibration of commercial instrument. Anal. Chem. 53, 778-788
33. Takakuwa, T., Konno, T. and Meguro, H. A. (1985) New standard substance for calibration of circular dichroism: ammonium d-10-camphorsulfonate. Anal. Sci. 1, 215-225
34. Hosseinkani, S., Ranjbar, B., Naderi-Manesh, H. and Nemat-Gorgani, M. (2004) Chemical modification of glucose oxidase: possible formation of molten globule-like intermediate structure. FEBS Lett. 561, 213-216
35. Guex, N. and Peitsch, M. C. (1997) SWISS-MODEL and the Swiss-pdb viewer: an environment for comparative protein modeling. Electrophoresis 18, 2714-2732
36. Branchini, B. R., Magyar, R. A., Murtiashaw, M. H., Anderson, S. M., Helgerson, L. C. and Zimmer, M. (1999) Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color. Biochemistry 38, 13223-13230
37. Ugarova, N. N. and Brovko, L. Y. (2002) Protein structure and bioluminescent spectra for firefly bioluminescence. Luminescence 17, 321-330
38. Viviani, V. R. (2002) The origin, diversity, and structure function relationships of insect luciferases. Cell. Mol. Life Sci. 59, 1833-1850
39. 175 in the control of luminescence decay. Biochem. J. 385, 572-580
40. 175) in structure and function of bacterial luciferase. FEBS Lett. 579, 4701-4706
41. Ugarova, N. N., Maloshenok, L. G., Uporov, I. V. and Koksharov, M. I. (2005) Bioluminescence spectra of native and mutant firefly luciferases as a function of pH. Biochemistry (Moscow) 70, 1262-1267
42. 356) in structure and bioluminescence spectra of firefly luciferase. J. Biol. Chem. 282, 8641-8647
43. Baggert, B., Roy, R., Momen, S., Morgan, S., Tisi, L., Morse, D. and Gillies, R. J. (2004) Thermostability of firefly luciferases affects efficiency of detection by in vivo bioluminescence. Mol. Imaging 3, 324-332