Emodin upregulates urokinase plasminogen activator, plasminogen activator inhibitor-1 and promotes wound healing in human fibroblasts

Vascular Pharmacology

Volumn 48, Issue 4-6, 2008, Pages 184-190

  Radha, K S ^a   Madhyastha, H K ^a   Nakajima, Y ^a   Omura, S ^a   Maruyama, M ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

Cell migration; Emodin; Fibrinolysis; Type 1 plasminogen activator inhibitor; Urokinase type plasminogen activator

Indexed keywords

ASCORBIC ACID; EMODIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PLASMINOGEN ACTIVATOR INHIBITOR 1; REACTIVE OXYGEN METABOLITE; UROKINASE;

ARTICLE; CELL MIGRATION; CONTROLLED STUDY; ENZYME ACTIVITY; FIBRINOLYSIS; FIBROBLAST; GEL MOBILITY SHIFT ASSAY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION; WESTERN BLOTTING; WOUND; WOUND HEALING; ZYMOGRAPHY;

ANTIOXIDANTS; BLOTTING, WESTERN; CELL MOVEMENT; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORETIC MOBILITY SHIFT ASSAY; EMODIN; FIBRIN; FIBRINOLYSIS; FIBROBLASTS; HUMANS; L-LACTATE DEHYDROGENASE; NF-KAPPA B; PLASMINOGEN ACTIVATOR INHIBITOR 1; PROTEIN KINASE INHIBITORS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STIMULATION, CHEMICAL; TETRAZOLIUM SALTS; THIAZOLES; UP-REGULATION; URINARY PLASMINOGEN ACTIVATOR; WOUND HEALING;

EID: 43449124394     PISSN: 15371891     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vph.2008.02.002     Document Type: Article

References (37)

1. Bass R., and Ellis V. Cellular mechanisms regulating non-haemostatic plasmin generation. Biochem. Soc. Trans. 30 (2002) 189-194
2. Bonello S., Zahringer C., BelAiba R.S., Djordjevic T., Hess J., Michiels C., Kietzmann T., and Gorlach A. Reactive oxygen species activate the HIF-1alpha promoter via a functional NFkappaB site. Arterioscler. Thromb. Vasc. Biol. 27 (2007) 755-761
3. Brown M., Bellon M., and Nicot C. Emodin and DHA potently increase arsenic trioxide interferon-alpha-induced cell death of HTLV-I-transformed cells by generation of reactive oxygen species and inhibition of Akt and AP-1. Blood 109 (2007) 1653-1659
4. Bugge T.H., Flick M.J., Danton M.J., Daugherty C.C., Romer J., Dano K., Carmeliet P., Collen D., and Degen J.L. Urokinase-type plasminogen activator is effective in fibrin clearance in the absence of its receptor or tissue-type plasminogen activator. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 5899-5904
5. Chithra P., Sajithlal G.B., and Chandrakasan G. Influence of aloe vera on collagen characteristics in healing dermal wounds in rats. Mol. Cell Biochem. 181 (1998) 71-76
6. Chithra P., Sajithlal G.B., and Chandrakasan G. Influence of aloe vera on the healing of dermal wounds in diabetic rats. J. Ethnopharmacol. 59 (1998) 195-201
7. Crippa M.P. Urokinase-type plasminogen activator. Int. J. Biochem. Cell Biol. 39 (2007) 690-694
8. Duvoix A., Delhalle S., Blasius R., Schnekenburger M., Morceau F., Fougere M., Henry E., Galteau M.M., Dicato M., and Diederich M. Effect of chemopreventive agents on glutathione S-transferase P1-1 gene expression mechanisms via activating protein 1 and nuclear factor kappaB inhibition. Biochem. Pharmacol. 68 (2004) 1101-1111
9. Fray T.R., Watson A.L., Croft J.M., Baker C.D., Bailey J., Sirel N., Tobias A., and Markwell P.J. A combination of aloe vera, curcumin, vitamin C, and taurine increases canine fibroblast migration and decreases tritiated water diffusion across canine keratinocytes in vitro. J. Nutr. 134 (2004) 2117S-2119S
10. Gandhari M., Arens N., Majety M., Dorn-Beineke A., and Hildenbrand R. Urokinase-type plasminogen activator induces proliferation in breast cancer cells. Int. J. Oncol. 28 (2006) 1463-1470
11. Idell S. Coagulation, fibrinolysis, and fibrin deposition in acute lung injury. Crit. Care Med. 31 (2003) S213-S220
12. Jing Y., Yang J., Wang Y., Li H., Chen Y., Hu Q., Shi G., Tang X., and Yi J. Alteration of subcellular redox equilibrium and the consequent oxidative modification of nuclear factor kappaB are critical for anticancer cytotoxicity by emodin, a reactive oxygen species-producing agent. Free Radic. Biol. Med. 40 (2006) 2183-2197
13. Kiian I., Tkachuk N., Haller H., and Dumler I. Urokinase-induced migration of human vascular smooth muscle cells requires coupling of the small GTPases RhoA and Rac1 to the Tyk2/PI3-K signalling pathway. Thromb. Haemost. 89 (2003) 904-914
14. Kohyama T., Liu X., Kim H.J., Kobayashi T., Ertl R.F., Wen F.Q., Takizawa H., and Rennard S.I. Prostacyclin analogs inhibit fibroblast migration. Am. J. Physiol. Lung Cell Mol. Physiol. 283 (2002) L428-L432
15. Kusch A., Tkachuk S., Haller H., Dietz R., Gulba D.C., Lipp M., and Dumler I. Urokinase stimulates human vascular smooth muscle cell migration via a phosphatidylinositol 3-kinase-Tyk2 interaction. J. Biol. Chem. 275 (2000) 39466-39473
16. Liang C.C., Park A.Y., and Guan J.L. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat. Protoc. 2 (2007) 329-333
17. Lijnen H.R. Pleiotropic functions of plasminogen activator inhibitor-1. J. Thromb. Haemost. 3 (2005) 35-45
18. Lin S.W., Ke F.C., Hsiao P.W., Lee P.P., Lee M.T., and Hwang J.J. Critical involvement of ILK in TGFbeta1-stimulated invasion/migration of human ovarian cancer cells is associated with urokinase plasminogen activator system. Exp. Cell Res. 313 (2007) 602-613
19. Lund L.R., Green K.A., Stoop A.A., Ploug M., Almholt K., Lilla J., Nielsen B.S., Christensen I.J., Craik C.S., Werb Z., Dano K., and Romer J. Plasminogen activation independent of uPA and tPA maintains wound healing in gene-deficient mice. Embo. J. 25 (2006) 2686-2697
20. Madhyastha H.K., Radha K.S., Sugiki M., Omura S., and Maruyama M. C-phycocyanin transcriptionally regulates uPA mRNA through cAMP mediated PKA pathway in human fibroblast WI-38 cells. Biochim. Biophys. Acta. 1760 (2006) 1624-1630
21. Maquerlot F., Galiacy S., Malo M., Guignabert C., Lawrence D.A., d'Ortho M.P., and Barlovatz-Meimon G. Dual role for plasminogen activator inhibitor type 1 as soluble and as matricellular regulator of epithelial alveolar cell wound healing. Am. J. Pathol. 169 (2006) 1624-1632
22. Mondino A., and Blasi F. uPA and uPAR in fibrinolysis, immunity and pathology. Trends Immunol. 25 (2004) 450-455
23. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65 (1983) 55-63
24. Muller M.J., Hollyoak M.A., Moaveni Z., Brown T.L., Herndon D.N., and Heggers J.P. Retardation of wound healing by silver sulfadiazine is reversed by Aloe vera and nystatin. Burns. 29 (2003) 834-836
25. Myohanen H., and Vaheri A. Regulation and interactions in the activation of cell-associated plasminogen. Cell Mol. Life Sci. 61 (2004) 2840-2858
26. Park M.J., Lee J.Y., Kwak H.J., Park C.M., Lee H.C., Woo S.H., Jin H.O., Han C.J., An S., Lee S.H., Chung H.Y., Park I.C., Hong S.I., and Rhee C.H. Arsenic trioxide (As2O3) inhibits invasion of HT1080 human fibrosarcoma cells: role of nuclear factor-kappaB and reactive oxygen species. J. Cell Biochem. 95 (2005) 955-969
27. Pawlak K., Pawlak D., and Mysliwiec M. Oxidative stress effects fibrinolytic system in dialysis uraemic patients. Thromb. Res. 117 (2006) 517-522
28. Quax P.H.A., van Muijen G.N.P., Pederson N., de Bart A.C.W., Schrauweb Y., Barlovatz-Meimon G., Blasi F., and Verheijen J.H. The plasminogen activator system in extracellular matrix degradation. Fibrinolysis 6 (1992) 41-44
29. Radha K.S., Sugiki M., Yoshida E., Harish Kumar M., Omura S., and Maruyama M. Iron-mediated stability of PAI-1 mRNA in adenocarcinoma cells-involvement of a mRNA-binding nuclear protein. Thromb. Res. 116 (2005) 255-263
30. Srinivas G., Babykutty S., Sathiadevan P.P., and Srinivas P. Molecular mechanism of emodin action: Transition from laxative ingredient to an antitumor agent. Med. Res. Rev. 27 (2007) 591-608
31. Tanaka F., Ogura N., and Abiko Y. Stimulation of plasminogen activator/plasmin system in gingival fibroblast cells by oxygen radicals. Arch. Oral Biol. 42 (1997) 263-270
32. Tang T., Yin L., Yang J., and Shan G. Emodin, an anthraquinone derivative from Rheum officinale Baill, enhances cutaneous wound healing in rats. Eur. J. Pharmacol. 567 (2007) 177-185
33. Tanski W.J., Fegley A.J., Roztocil E., and Davies M.G. Domain-dependent action of urokinase on smooth muscle cell responses. J. Vasc. Surg. 39 (2004) 214-222
34. Vulin A.I., and Stanley F.M. Oxidative stress activates the plasminogen activator inhibitor type 1 (PAI-1) promoter through an AP-1 response element and cooperates with insulin for additive effects on PAI-1 transcription. J. Biol. Chem. 279 (2004) 25172-25178
35. Wang Z., Sosne G., and Kurpakus-Wheater M. Plasminogen activator inhibitor-1 (PAI-1) stimulates human corneal epithelial cell adhesion and migration in vitro. Exp. Eye Res. 80 (2005) 1-8
36. Watanabe M., Kondo S., Mizuno K., Yano W., Nakao H., Hattori Y., Kimura K., and Nishida T. Promotion of corneal epithelial wound healing in vitro and in vivo by annexin A5. Invest. Ophthalmol. Vis. Sci. 47 (2006) 1862-1868
37. Yamamoto K., and Loskutoff D.J. Fibrin deposition in tissues from endotoxin-treated mice correlates with decreases in the expression of urokinase-type but not tissue-type plasminogen activator. J. Clin. Invest. 97 (1996) 2440-2451