Crystal structure of schistatin, a disintegrin homodimer from saw-scaled viper (Echis carinatus) at 2.5 Å resolution

Journal of Molecular Biology

Volumn 341, Issue 3, 2004, Pages 829-837

  Bilgrami, Sameeta ^a   Tomar, Shailly ^a   Yadav, Savita ^a   Kaur, Punit ^a   Kumar, Janesh ^a   Jabeen, Talat ^a   Sharma, Sujata ^a   Singh, Tej P ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Arg Gly Asp loop; crystal structure; disintegrin; homodimer; schistatin

Indexed keywords

DISINTEGRIN; PROTEIN SUBUNIT; SCHISTATIN; UNCLASSIFIED DRUG; VIPER VENOM;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DISULFIDE BOND; ECHIS CARINATUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN ISOLATION; PROTEIN MOTIF; PROTEIN QUATERNARY STRUCTURE; PROTEIN STRUCTURE; SNAKE; STRUCTURE ANALYSIS;

ECHIS CARINATUS;

EID: 4344607978     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2004.06.048     Document Type: Article

References (61)

1. R.J. Gould, M.A. Polokoff, P.A. Friedman, T.F. Huang, J.C. Holt, J.J. Cook, and S. Niewiarowski Disintegrins: a family of integrin inhibitory proteins from viper venoms Proc. Soc. Expt. Biol. Med. 195 1990 168 171
2. Z.R. Gan, R.J. Gould, J.W. Jacobs, P.A. Freidman, and M.A. Polokof Echistatin: a potent platelet aggregation inhibitor from the venom of the viper Echis carinatus J. Biol. Chem. 263 1988 19827 19832
3. L.C. Au, J.S. Chou, K.J. Chang, G.W. Teh, and S.B. Lin Nucleotide sequence of a full-length cDNA encoding a common precursor of platelet aggregation inhibitor and hemorrhagic protein from Calloselasma rhodostoma venom Biochim. Biophys. Acta 1173 1993 243 245
4. M. Kishimoto, and T. Takahashi Molecular cloning and sequence analysis of cDNA encoding flavoridin, a disintegrin from the venom of Trimeresurus flavoviridis Toxicon 40 2002 1033 1040
5. T.F. Huanf, J.C. Holt, H. Lukasiewicz, and S. Niewiarowski Trigramin: a low molecular weight peptide inhibiting fibrinogen interaction with platelet receptors expressed glycoprotein IIb-IIIa complex J. Biol. Chem. 262 1987 16157 16163
6. T.F. Huang, J.R. Sheu, C.M. Teng, S.W. Chen, and C.S. Liu Triflavin, an antiplatelet Arg-Gly-Asp-containing peptide, is a specific antagonist of platelet membrane glycoprotein IIb-IIIa complex J. Biochem. 109 1991 328 334
7. M.S. Dennis, W.J. Henzel, R.M. Pitti, M.T. Lipari, M.A., Napier, and T.A. Deisher Platelet glycoprotein IIb-IIIa protein antagonists from snake venoms: evidence for a family of platelet-aggregation inhibitors Proc. Natl Acad. Sci. USA 87 1990 2471 2475
8. R.M. Scarborough, J.W. Rose, M.A. Hsu, D.R. Phillips, V.A. Fried, and A.M. Campbell Barbourin. A GPIIb-IIIa-specific integrin antagonist from the venom of Sistrurus m. barbouri J. Biol. Chem. 266 1991 9359 9362
9. K. Maruyama, T. Kawasaki, Y. Sakai, Y. Taniuchi, M. Shimizu, H. Kawashima, and T. Takenaka Isolation and amino acid sequence of flavostatin, a novel disintegrin from the venom of Trimeresurus flavoviridis Peptides 18 1997 73 78
10. T.F. Huang, C.Z. Liu, C.H. Ouyang, and C.M. Teng Halysin, an antiplatelet Arg-Gly-Asp-containing snake venom peptide, as fibrinogen receptor antagonist Biochem. Pharmacol. 42 1991 1209 1219
11. J. Williams, B. Rucinski, J. Holt, and S. Niewiarowski Elegantin and albolabrin purified peptides from viper venoms: homologies with the RGDS domain of fibrinogen and von Willebrand factor Biochim. Biophys. Acta 1039 1990 81 89
12. B.H. Chao, J.A. Jakubowski, B. Savage, E.P. Chow, U.M. Marzec, L.A. Harker, and J.M. Maraganore Agkistrodon piscivorus piscivorus platelet aggregation inhibitor: a potent inhibitor of platelet activation Proc. Natl Acad. Sci. USA 86 1989 8050 8054
13. C. Marcinkiewicz, J.J. Calvete, M.M. Marcinkiewicz, M. Raida, S. Vijay-Kumar, and Z. Huang EC3 a novel MLD dependant disintegrin from Echis carinatus, is a potent antagonist for α4 integrins J. Biol. Chem. 274 1999 12468 12473
14. C. Marcinkiewicz, Y. Taooka, Y. Yokosaki, J.J. Calvete, M.M. Marcinkiewicz, and R.R. Lobb Inhibitory effects of MLDG-containing heterodimeric disintegrins reveal distinct structural requirements for interaction of the integrin alpha 9beta 1 with VCAM-1, tenascin-C and osteopontin J. Biol. Chem. 275 2000 31930 31937
15. C. Marcinkiewicz, J.J. Calvete, S. Vijay-Kumar, M.M. Marcinkiewicz, M. Raida, and P. Schick Structural and functional characterization of EMF10, a heterodimeric disintegrin from Eristocophis macmahoni venom that selectively inhibits alpha5 beta1 integrin Biochemistry 38 1999 13302 13309
16. D. Okuda, and T. Morita Purification and characterization of a new RGD/KGD-containing dimeric disintegrin, piscivostatin, from the venom of Agkistrodon piscivorus piscivorus: the unique effect of piscivostatin on platelet aggregation J. Biochem. 130 2001 407 415
17. A. Gasmi, N. Sriari, S. Guermazi, H. Dkhil, H. Karoui, and M.E. Ayeb Amino acid structure and characterization of a heterodimeric disintegrin from Vipera lebetina venom Biochim. Biophys. Acta 1547 2001 51 56
18. D. Okuda, H. Koike, and T. Morita A new gene structure of the disintegrin family: a subunit of dimeric disintegrin has a short coding region Biochemistry 41 2002 14248 14254
19. J.J. Calvete, J.W. Fow, A. Agelan, S. Niewiarowski, and C. Marcinkiewicz The presence of the WGD motif in CC8 heterodimeric disintegrin increases its inhibitory effect on alphaII(b)beta3, alpha(v)beta3 and alpha5beta1 integrins Biochemistry 41 2002 2014 2021
20. M. Trikha, Y.A. De Clarke, and F.S. Markland Contortrostatin a snake venom disintegrin, inhibits β1 integrin mediated human metastatic melanoma cell adhesion and blocks experimental metastasis Cancer Res. 54 1994 4993 4998
21. Q. Zhou, P. Hu, M.R. Ritter, S.D. Swenson, S. Argounova, A.L. Epstein, and F.S. Markland Molecular cloning and functional expression of contortrostatin, a homodimeric disintegrin from southern copperhead snake venom Arch. Biochem. Biophys. 375 2000 278 288
22. M.D. Pierschbacher, and E. Rouslahti Arg-Gly-Asp: a versatile cell recognition signal Cell 44 1986 517 518
23. R. Hynes Integrins: versatility, modulation, and signaling in cell adhesion Cell 69 1992 11 25
24. R.O. Hynes Integrins: a family of cell surface receptors Cell 48 1987 549 554
25. D. Sheppard In vivo functions of integrins: lessons from null mutations in mice Matrix Biol. 9 2000 203 209
26. T.V. Byzova, R. Rabbani, S.E. D'Souza, and E.F. Plow Role of integrin α5β3 in vascular biology Thromb. Heamost. 80 1998 726 734
27. P.C. Brooks, R.A.F. Clark, and D.A. Cheresh Requirement of vascular integrin α5β3 for angiogenesis Science 264 1994 569 571
28. M. Friedlander, P.C. Brooks, R.W. Shaffer, C.M. Kincaid, J.A. Varner, and D.A. Cheresh Definition of two angiogenic pathways by distinct α5 integrins Science 270 1995 1500 1502
29. S. Hapke, H. Kessler, B. Luber, A. Benge, P. Hutzler, and H. Hofler Ovarian cancer cell proliferation and motility is induced by engagement of integrin alpha(v)beta3/Vitronectin interaction Biol. Chem. 384 2003 1073 1083
30. G.C. Tucker Alpha v integrin inhibitors and cancer therapy Curr. Opin. Investig. Drugs 4 2003 722 731
31. D. Schuppan, and M. Ocker Integrin-mediated control of cell growth Hepatology 38 2003 289 291
32. D.R. Philips, I.F. Charo, L.V. Parise, and L.A. Fitzgerald The platelet membrane glycoprotein IIb-IIIa complex Blood 71 1988 831 843
33. E.F. Plow, and M.H. Ginsberg Cellular adhesion GP IIb-IIIa as prototypic adhesion receptor Prog. Hemost. Thromb. 9 1989 117 156
34. E.A. Clark, M. Trikha, F.S. Markland, and J.S. Brugge Structurally distinct disintegrins contortrostatin and multisquamatin differentially regulate platelet tyrosine phosphorylation J. Mol. Biol. 269 1994 21940 21943
35. P. Edman Sequence determination Mol. Biol. Biochem. Biophys. 8 1970 211 255
36. A. Bairoch, and R. Apweiler The SWISS-PROT protein sequence data bank and its new supplement TREMBL Nucl. Acids Res. 24 1996 21 25
37. C. Marcinkiewicz, S. Vijay-Kumar, M.A. McLane, and S. Niewiarowski Significance of RGD loop and C-terminal domain of echistatin for recognition of alphaIIb beta3 and alpha(v) beta3 integrins and expression of ligand-induced binding site Blood 90 1997 1565 1575
38. 1 integrins J. Biol. Chem. 274 1999 37809 37814
39. R.M. Scarborough, J.W. Rose, M.A. Naughton, R.D. Phillips, L. Nannizzi, and A. Arfsten Characterization of the integrin specificities of disintegrin isolated from American pit viper venoms J. Biol. Chem. 268 1993 1058 1065
40. M.A. McLane, S. Vijay-Kumar, C. Marcinkiewicz, J.J. Calvete, and S. Niewiarowski Importance of the structure of the RGD containing loop in the disintegrins echistatin and eristostatin for recognition of alpha IIb beta 3 and alpha v beta 3 integrins FEBS Letters 391 1996 139 143
41. 2+ cation Biochem. J. 335 1998 247 257
42. R.A. Laskowski, M.W. MacArthur, D.S. Moss, and J.M. Thornton PROCHECK: a program to check the stereochemical quality of protein structures J. Appl. Crystallog. 26 1993 283 291
43. G.N. Ramachandran, and V. Sasisekharan Conformation of polypeptides and proteins Advan. Protein Chem. 23 1968 383 438
44. J.J. Calvete, M. Jurgens, C. Marcinkiewicz, A. Romero, M. Schrader, and S. Niewiarowski Disulfide-bond pattern and molecular modelling of the dimeric disintegrin EMF-10, a potent and selective integrin alpha5beta1 antagonist from Eristocophis macmahoni venom Biochem. J. 345 2000 573 581
45. J. Jasti, M. Paramasivam, A. Srinivasan, and T.P. Singh Crystal structure of echicetin from Echis carinatus (Indian Saw-scaled Viper) at 2.4 Å resolution J. Mol. Biol. 335 2004 167 176
46. M. Adler, R.A. Lazarus, M.S. Dennis, and G. Wagner Solution structure of Kistrin, potent platelet aggregation Inhibitor and GP IIb-IIIa antagonist Science 253 1991 445 448
47. V. Saudek, R.A. Atkinson, P. Lepage, and J.T. Pelton Three dimensional structure of echistatin, the smallest active RGD protein Biochemistry 30 1991 7369 7372
48. H. Senn, and W. Klaus The nuclear magnetic resonance solution structure of flavoridin, an antagonist of the platelet GP IIa-IIIa receptor J. Mol. Biol. 232 1993 907 925
49. Y. Fuji, D. Okuda, Z. Fujimoto, K. Horii, T. Morita, and H. Mizuno Crystal structure of trimestatin, a disintegrin containing cell adhesion recognition motif RGD J. Mol. Biol. 332 2003 1115 1122
50. M. Pfaff, K. Tangemann, B. Muller, M. Gurrath, G. Muller, and H. Kessler Selective recognition of cyclic RGD peptides of NMR defined conformation by alpha IIb beta 3, alpha V beta 3, and alpha 5 beta 1 integrins J. Biol. Chem. 269 1994 20233 20238
51. M.R. Ritter, and F.S. Markland Jr Differential regulation of tyrosine phosphorylation in tumor cells by contortrostatin, a homodimeric disintegrin and monomeric disintegrins echistatin and flavoridin Toxicon 39 2001 283 289
52. T. Hato, N. Pampori, and S.J. Shattil Complementary roles for receptor clustering and conformational change in the adhesive and signaling functions of integrin alphaIIb beta3 J. Cell Biol. 141 1998 1685 1695
53. Collaborative Computational Project Number 4 The CCP4 suite: programs for protein crystallography Acta Crystallog. sect. D 50 1994 760 763
54. Z. Otwinowski, and W. Minor Processing of X-ray diffraction data collection in oscillation mode Methods Enzymol. 276 1997 307 326
55. T.C. Terwilliger, and J. Brendzen Automated MAD and MIR structure solution Acta Crystallog. sect. D 55 1999 849 861
56. T.C. Terwilliger Maximum-likelihood density modification Acta Crystallog. sect. D 56 2000 965 972
57. A. Perrakis, R. Morris, and V.S. Lamzin Automated protein model building combined with iterative structure refinement Nature Struct. Biol. 6 1999 458 463
58. G.N. Murshudov, A.A. Vagin, and E.J. Dodson Refinement of macromolecular structures by the maximum-likelihood method Acta Crystallog. sect. D 53 1997 240 255
59. T.A. Jones, J.Y. Zou, S.W. Cowan, and M. Kjeldgaard Improved methods for building protein models in electron density maps and the location of errors in these models Acta Crystallog. sect. A 47 1991 110 119
60. P.J. Kraulis "MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures" J. Appl. Crystallog. 24 1991 946 950
61. E.A. Merritt, and M.E.P. Murphy Raster3D version 2.0: a program for photorealistic molecular graphics Acta Crystallog. sect. D 50 1994 869 873