Purification and characterization of a new RGD/KGD-containing dimeric disintegrin, piscivostatin, from the venom of Agkistrodon piscivorus piscivorus: The unique effect of piscivostatin on platelet aggregation

Journal of Biochemistry

Volumn 130, Issue 3, 2001, Pages 407-415

  Okuda, D ^a   Morita, T ^a  

^a MEIJI PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

ADP induced platelet aggregation; Disintegrin; Laser light scattering; Platelet shape change; Snake venom

Indexed keywords

ADENOSINE DIPHOSPHATE; AMINO ACID; ARGININE; ASPARTIC ACID; BINDING PROTEIN; DIMER; DISINTEGRIN; ECHISTATIN; FIBRINOGEN; FIBRINOGEN RECEPTOR; GLYCINE; LYSINE; MONOCLONAL ANTIBODY; PISCIVOSTATIN; SNAKE VENOM; UNCLASSIFIED DRUG;

AGKISTRODON; ALPHA CHAIN; AMINO ACID SEQUENCE; ARTICLE; BETA CHAIN; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DOSE RESPONSE; HUMAN; HUMAN CELL; LIGHT SCATTERING; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN PURIFICATION; THROMBOCYTE ACTIVATION; THROMBOCYTE AGGREGATION; THROMBOCYTE SHAPE; THROMBOCYTOPOIESIS;

AGKISTRODON; AGKISTRODON PISCIVORUS PISCIVORUS; SERPENTES;

EID: 0034854834     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003000     Document Type: Article

References (36)

1. The tetrapeptide analogue of the cell attachment site of fibronectin inhibits platelet aggregation and fibrinogen binding to activated platelets
2. The effect of Arg-Gly-Asp-containing peptides on fibrinogen and von Willebrand factor binding to platelets
3. Arginylglycyl-aspartic acid (RGD): A cell adhesion motif
4. Disintegrins: A family of integrin inhibitory proteins from viper venoms
5. Disintegrins: The naturally-occurring antagonists of platelet fibrinogen receptor
6. Disintegrins and other naturally occurring antagonists of platelet fibrinogen receptors
7. Echistatin inhibits pp72syk and pp125FAK phosphorylation in fibrinogen-adherent platelets
8. Echistatin induces decrease of pp125FAK phosphorylation, disassembly of actin cytoskeleton and focal adhesions, and detachment of fibronectin-adherent melanoma cells
9. Echistatin. A potent platelet aggregation inhibitor from the venom of the viper, Echis carinatus
10. Barbourin. A GPIIb-IIIa-specific integrin antagonist from the venom of Sistrurus m. barbouri
11. Trigramin. A low molecular weight peptide inhibiting fibrinogen interaction with platelet receptors expressed on glycoprotein IIb-IIIa complex
12. Platelet glycoprotein IIb-IIIa protein antagonists from snake venoms: Evidence for a family of platelet-aggregation inhibitors
13. Characterization and platelet inhibitory activity of bitistatin, a potent arginine-glycine-aspartic acid-containing peptide from the venom of the viper Bitis arietans
14. The disulfide bridge pattern of snake venom disintegrins, flavoridin and echistatin
15. 1H-NMR, circulardichroism and Raman spectroscopy
16. The nuclear magnetic resonance solution structure of flavoridin, an antagonist of the platelet GP IIb-IIIa receptor
17. Cysteine pairing in the glycoprotein IIbIIIa antagonist kistrin using NMR, chemical analysis, and structure calculations
18. The disulphide bond pattern of bitistatin, a disintegrin isolated from the venom of the viper Bitis arietans
19. Purification and characterization of platelet aggregation inhibitors from snake venoms
20. Molecular cloning and functional expression of contortrostatin, a homodimeric disintegrin from southern copperhead snake venom
21. Ussuristatin 2, a novel KGD-bearing disintegrin from Agkistrodon ussuriensis venom
22. Structural and functional characterization of EMF10, a heterodimeric disintegrin from Eristocophis macmahoni venom that selectively inhibits α 5 β 1 integrin
23. A novel regulatory epitope defined by a murine monoclonal antibody to the platelet GPIIb-IIIa complex (α IIb β 3 integrin)
24. Platelet membrane glycoprotein IIb-IIIa complex: Purification, characterization, and reconstitution into phospholipid vesicles
25. Triflavin, an antiplatelet Arg-Gly-Asp-containing peptide, is a specific antagonist of platelet membrane glycoprotein IIb-IIIa complex
26. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
27. Detection of platelet aggregates with a particle counting method using light scattering
28. EC3, a novel heterodimeric disintegrin from Echis carinatus venom, inhibits α4 and α5 integrins in an RGD-independent manner
29. Agkistrodon piscivorus piscivorus platelet aggregation inhibitor: A potent inhibitor of platelet activation
30. Arg-Gly-Asp-dependent occupancy of GPIIb/IIIa by applaggin: Evidence for internalization and cycling of a platelet integrin
31. Disulphide-bond pattern and molecular modelling of the dimeric disintegrin EMF-10, a potent and selective integrin α5β1 antagonist from Eristocophis macmahoni venom
32. Determination of amino acid sequence of dimeric disintegrin
33. Complementary roles for receptor clustering and conformational change in the adhesive and signaling functions of integrin αIIb β3
34. Structurally distinct disintegrins contortrostatin and multisquamatin differentially regulate platelet tyrosine phosphorylation
35. Integrin cytoplasmic tyrosine motif is required for outside-in αIIbβ3 signalling and platelet function