The importance of growth factors for preimplantation embryo development and in-vitro culture

Current Opinion in Obstetrics and Gynecology

Volumn 20, Issue 3, 2008, Pages 292-304

  Richter, Kevin S ^a  

^a Shady Grove Fertility Reproductive Science Center   (United States)

Author keywords

Embryo culture; Growth factor; In vitro fertilization; Preimplantation development

Indexed keywords

ACTIVIN A; COLONY STIMULATING FACTOR; COLONY STIMULATING FACTOR 1; EPIDERMAL GROWTH FACTOR; FIBROBLAST GROWTH FACTOR; FIBRONECTIN; GROWTH FACTOR; HEPARIN BINDING EPIDERMAL GROWTH FACTOR; INHIBIN; LEUKEMIA INHIBITORY FACTOR; SOMATOMEDIN B; SOMATOMEDIN C; THROMBOCYTE ACTIVATING FACTOR; TRANSFORMING GROWTH FACTOR ALPHA; VASCULOTROPIN;

AUTOCRINE EFFECT; BECKWITH WIEDEMANN SYNDROME; BLASTOCYTE; COCULTURE; DEVELOPMENTAL DISORDER; EMBRYO; EMBRYO CULTURE; EMBRYO DEVELOPMENT; EMBRYO GROWTH; ENDOMETRIUM; FERTILIZATION; FERTILIZATION IN VITRO; FETUS DEVELOPMENT; GENE DISRUPTION; GENETIC RISK; GRANULOSA CELL; HAPPY PUPPET SYNDROME; HUMAN; LUTEAL CELL; NONHUMAN; OVARY FOLLICLE; OVARY FOLLICLE CELL; OVIDUCT; PREGNANCY RATE; PREIMPLANTATION EMBRYO; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVIEW; SHEEP;

ANIMALS; BLASTOCYST; EMBRYO CULTURE TECHNIQUES; EMBRYONIC DEVELOPMENT; FEMALE; FETAL DEVELOPMENT; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; PREGNANCY; RANDOMIZED CONTROLLED TRIALS AS TOPIC; RECEPTORS, GROWTH FACTOR;

EID: 43149123533     PISSN: 1040872X     EISSN: None     Source Type: Journal    
DOI: 10.1097/GCO.0b013e3282fe743b     Document Type: Review

References (220)

1. Gardner DK, Lane M. Culture of viable human blastocysts in defined sequential serum-free media. Hum Reprod 1998; 13 (Suppl 3):148-159.
2. Muttukrishna S, Groome N, Ledger W. Gonadotropic control of secretion of dimeric inhibins and activin A by human granulosa-luteal cells in vitro. J Assist Reprod Genet 1997; 14:566-574.
3. Petraglia F, Florio P, Luisi S, et al. Expression and secretion of inhibin and activin in normal and neoplastic uterine tissues. High levels of serum activin A in women with endometrial and cervical carcinoma. J Clin Endocrinol Metab 1998; 83:1194-1200.
4. He ZY, Liu HC, Mele CA, et al. Expression of inhibin/activin subunits and their receptors and binding proteins in human preimplantation embryos. J Assist Reprod Genet 1999; 16:73-80.
5. Kauma SW, Aukerman SL, Eierman D, Turner T. Colony-stimulating factor-1 and c-fms expression in human endometrial tissues and placenta during the menstrual cycle and early pregnancy. J Clin Endocrinol Metab 1991; 73:746-751.
6. Sharkey AM, Dellow K, Blayney M, et al. Stage-specific expression of cytokine and receptor messenger ribonucleic acids in human preimplantation embryos. Biol Reprod 1995; 53:974-981.
7. Morishige K, Kurachi H, Amemiya K, et al. Menstrual stage-specific expression of epidermal growth factor and transforming growth factor-alpha in human oviduct epithelium and their role in early embryogenesis. Endocrinology 1993; 133:199-207.
8. Chegini N, Zhao Y, McLean FW. Expression of messenger ribonucleic acid and presence of immunoreactive proteins for epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha) and EGF/TGF alpha receptors and 125I-EGF binding sites in human fallopian tube. Biol Reprod 1994; 50:1049-1058.
9. Kurachi H, Morishige K, Imai T, et al. Expression of epidermal growth factor and transforming growth factor-alpha in fallopian tube epithelium and their role in embryogenesis. Horm Res 1994; 41 (Suppl 1):48-154.
10. Sato A, Bo M, Otani T, Mochizuki M. Expression of epidermal growth factor, transforming growth factor-alpha and epidermal growth factor receptor messenger RNA in human endometrium and endometrial carcinoma. Nippon Sanka Fujinka Gakkai Zasshi 1995; 47:473-478.
11. Chia CM, Winston RM, Handyside AH. EGF, TGF-alpha and EGFR expression in human preimplantation embryos. Development 1995; 121:299-307.
12. Smotrich DB, Stillman RJ, Widra EA, et al. Immunocytochemical localization of growth factors and their receptors in human preembryos and fallopian tubes. Hum Reprod 1996; 11:184-190.
13. Jiang Q, Chen S, Xing F. Expression of epidermal growth factor receptor in human preimplantation embryos. Zhonghua Fu Chan Ke Za Zhi 2000; 35:468-469.
14. Watson R, Anthony F, Pickett M, et al. Reverse transcription with nested polymerase chain reaction shows expression of basic fibroblast growth factor transcripts in human granulosa and cumulus cells from in vitro fertilisation patients. Biochem Biophys Res Commun 1992; 187:1227-1231.
15. Di Blasio AM, Vigano P, Cremonesi L, et al. Expression of the genes encoding basic fibroblast growth factor and its receptor in human granulosa cells. Mol Cell Endocrinol 1993; 96:R7-R11.
16. Seli E, Zeyneloglu HB, Senturk LM, et al. Basic fibroblast growth factor: peritoneal and follicular fluid levels and its effect on early embryonic development. Fertil Steril 1998; 69:1145-1148.
17. Familiari G, Verlengia C, Nottola SA, et al. Heterogeneous distribution of fibronectin, tenascin-C, and laminin immunoreactive material in the cumulus-corona cells surrounding mature human oocytes from IVF-ET protocols-evidence that they are composed of different subpopulations: an immunohistochemical study using scanning confocal laser and fluorescence microscopy. Mol Reprod Dev 1996; 43:392-402.
18. Hornung D, Lebovic DI, Shifren JL, et al. Vectorial secretion of vascular endothelial growth factor by polarized human endometrial epithelial cells. Fertil Steril 1998; 69:909-915.
19. Zhao Y, Chegini N. Human fallopian tube expresses granulocyte-macrophage colony stimulating factor (GM-CSF) and GM-CSF alpha and beta receptors and contain immunoreactive GM-CSF protein. J Clin Endocrinol Metab 1994; 79:662-665.
20. Zhao Y, Chegini N. The expression of granulocyte macrophage-colony stimulating factor (GM-CSF) and receptors in human endometrium. Am J Reprod Immunol 1999; 42:303-311.
21. Sjoblom C, Wikland M, Robertson SA. Granulocyte-macrophage colony-stimulating factor (GM-CSF) acts independently of the beta common subunit of the GM-CSF receptor to prevent inner cell mass apoptosis in human embryos. Biol Reprod 2002; 67:1817-1823.
22. Sun XX, Gemzell-Danielsson K, Li HZ, et al. Expression of heparin-binding epidermal growth factor-like growth factor and its receptors in the human fallopian tube and endometrium after treatment with mifepristone. Fertil Steril 2006; 85:171-178.
23. Yoo HJ, Barlow DH, Mardon HJ. Temporal and spatial regulation of expression of heparin-binding epidermal growth factor-like growth factor in the human endometrium: a possible role in blastocyst implantation. Dev Genet 1997; 21:102-108.
24. Leach RE, Khalifa R, Ramirez ND, et al. Multiple roles for heparin-binding epidermal growth factor-like growth factor are suggested by its cell-specific expression during the human endometrial cycle and early placentation. J Clin Endocrinol Metab 1999; 84:3355-3363.
25. Stavreus-Evers A, Aghajanova L, Brismar H, et al. Co-existence of heparin-binding epidermal growth factor-like growth factor and pinopodes in human endometrium at the time of implantation. Mol Hum Reprod 2002; 8:765-769.
26. Chobotova K, Spyropoulou I, Carver J, et al. Heparin-binding epidermal growth factor and its receptor ErbB4 mediate implantation of the human blastocyst. Mech Dev 2002; 119:137-144.
27. Wang TH, Chang CL, Wu HM, et al. Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development. Fertil Steril 2006; 86:1392-1401.
28. Pfeifer TL, Chegini N. Immunohistochemical localization of insulin-like growth factor (IGF-I), IGF-I receptor, and IGF binding proteins 1-4 in human fallopian tube at various reproductive stages. Biol Reprod 1994; 50:281-289.
29. Lighten AD, Moore GE, Winston RM, Hardy K. Routine addition of human insulin-like growth factor-I ligand could benefit clinical in-vitro fertilization culture. Hum Reprod 1998; 13:3144-3150.
30. Tang XM, Rossi MJ, Masterson BJ, Chegini N. Insulin-like growth factor I (IGF-I), IGF-I receptors, and IGF binding proteins 1-4 in human uterine tissue: tissue localization and IGF-I action in endometrial stromal and myometrial smooth muscle cells in vitro. Biol Reprod 1994; 50:1113-1125.
31. Lighten AD, Hardy K, Winston RM, Moore GE. Expression of mRNA for the insulin-like growth factors and their receptors in human preimplantation embryos. Mol Reprod Dev 1997; 47:134-139.
32. Liu HC, He ZY, Mele CA, et al. Human endometrial stromal cells improve embryo quality by enhancing the expression of insulin-like growth factors and their receptors in cocultured human preimplantation embryos. Fertil Steril 1999; 71:361-367.
33. Ramasharma K, Li CH. Human pituitary and placental hormones control human insulin-like growth factor II secretion in human granulosa cells. Proc Natl Acad Sci U S A 1987; 84:2643-2647.
34. Giudice LC, Lamson G, Rosenfeld RG, Irwin JC. Insulin-like growth factor-II (IGF-II) and IGF binding proteins in human endometrium. Ann N Y Acad Sci 1991; 626:295-307.
35. Giudice LC, Dsupin BA, Jin IH, et al. Differential expression of messenger ribonucleic acids encoding insulin-like growth factors and their receptors in human uterine endometrium and decidua. J Clin Endocrinol Metab 1993; 76:1115-1122.
36. Vanttinen T, Liu J, Hyden-Granskog C, et al. Regulation of immunoreactive inhibin A and B secretion in cultured human granulosa-luteal cells by gonadotropins, activin A and insulin-like growth factor type-1 receptor. J Endocrinol 2000; 167:289-294.
37. Davis SR, Krozowski Z, McLachlan RI, Burger HG. Inhibin gene expression in the human corpus luteum. J Endocrinol 1987; 115:R21-R23.
38. Wang HZ, Lu SH, Han XJ, et al. Control of inhibin production by dispersed human luteal cells in vitro. Reprod Fertil Dev 1992; 4:67-75.
39. Piccinni MP, Scaletti C, Mavilia C, et al. Production of IL-4 and leukemia inhibitory factor by T cells of the cumulus oophorus: a favorable microenvironment for preimplantation embryo development. Eur J Immunol 2001; 31:2431-2437.
40. Keltz MD, Attar E, Buradagunta S, et al. Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube. Am J Obstet Gynecol 1996; 175:1611-1619.
41. Arici A, Engin O, Attar E, Olive DL. Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in human endometrium. J Clin Endocrinol Metab 1995; 80:1908-1915.
42. Cullinan EB, Abbondanzo SJ, Anderson PS, et al. Leukemia inhibitory factor (LIF) and LIF receptor expression in human endometrium suggests a potential autocrine/paracrine function in regulating embryo implantation. Proc Natl Acad Sci U S A 1996; 93:3115-3120.
43. Tsai HD, Chang CC, Hsieh YY, Lo HY. Leukemia inhibitory factor expression in different endometrial locations between fertile and infertile women throughout different menstrual phases. J Assist Reprod Genet 2000; 17:415-418.
44. van Eijk MJ, Mandelbaum J, Salat-Baroux J, et al. Expression of leukaemia inhibitory factor receptor subunits LIFR beta and gp130 in human oocytes and preimplantation embryos. Mol Hum Reprod 1996; 2:355-360.
45. Wanggren K, Lalitkumar PG, Hambiliki F, et al. Leukaemia inhibitory factor receptor and gp130 in the human fallopian tube and endometrium before and after mifepristone treatment and in the human preimplantation embryo. Mol Hum Reprod 2007; 13:391-397. Reports the identification of LIF receptors in preimplantation embryos, including in all cells of blastocysts.
46. Salmassi A, Zhang Z, Schmutzler AG, et al. Expression of mRNA and protein of macrophage colony-stimulating factor and its receptor in human follicular luteinized granulosa cells. Fertil Steril 2005; 83:419-425.
47. Roudebush WE, Purnell ET, Stoddart NR, Fleming SD. Embryonic platelet-activating factor: temporal expression of the ligand and receptor. J Assist Reprod Genet 2002; 19:72-78.
48. Boehm KD, Daimon M, Gorodeski IG, et al. Expression of the insulin-like and platelet-derived growth factor genes in human uterine tissues. Mol Reprod Dev 1990; 27:93-101.
49. Desai NN, Goldfarb JM. Growth factor/cytokine secretion by a permanent human endometrial cell line with embryotrophic properties. J Assist Reprod Genet 1996; 13:546-550.
50. Osterlund C, Wramsby H, Pousette A. Temporal expression of platelet-derived growth factor (PDGF)-A and its receptor in human preimplantation embryos. Mol Hum Reprod 1996; 2:507-512.
51. el-Danasouri I, Frances A, Westphal LM. Immunocytochemical localization of transforming growth factor-alpha and epidermal growth factor receptor in human fallopian tubes and cumulus cells. Am J Reprod Immunol 1993; 30:82-87.
52. Kamat BR, Brown LF, Manseau EJ, et al. Expression of vascular permeability factor/vascular endothelial growth factor by human granulosa and theca lutein cells. Role in corpus luteum development. Am J Pathol 1995; 146:157-165.
53. Neulen J, Yan Z, Raczek S, et al. Human chorionic gonadotropin-dependent expression of vascular endothelial growth factor/vascular permeability factor in human granulosa cells: importance in ovarian hyperstimulation syndrome. J Clin Endocrinol Metab 1995; 80:1967-1971.
54. Neulen J, Raczek S, Pogorzelski M, et al. Secretion of vascular endothelial growth factor/vascular permeability factor from human luteinized granulosa cells is human chorionic gonadotrophin dependent. Mol Hum Reprod 1998; 4:203-206.
55. Gordon JD, Mesiano S, Zaloudek CJ, Jaffe RB. Vascular endothelial growth factor localization in human ovary and fallopian tubes: possible role in reproductive function and ovarian cyst formation. J Clin Endocrinol Metab 1996; 81:353-359.
56. Sugino N, Kashida S, Takiguchi S, et al. Expression of vascular endothelial growth factor and its receptors in the human corpus luteum during the menstrual cycle and in early pregnancy. J Clin Endocrinol Metab 2000; 85:3919-3924.
57. Lam PM, Briton-Jones C, Cheung CK, et al. Vascular endothelial growth factor in the human oviduct: localization and regulation of messenger RNA expression in vivo. Biol Reprod 2003; 68:1870-1876.
58. Torry DS, Holt VJ, Keenan JA, et al. Vascular endothelial growth factor expression in cycling human endometrium. Fertil Steril 1996; 66:72-80.
59. Zhang L, Scott PA, Turley H, et al. Validation of antivascular endothelial growth factor (anti-VEGF) antibodies for immunohistochemical localization of VEGF in tissue sections: expression of VEGF in the human endometrium. J Pathol 1998; 185:402-408.
60. Sugino N, Kashida S, Karube-Harada A, et al. Expression of vascular endothelial growth factor (VEGF) and its receptors in human endometrium throughout the menstrual cycle and in early pregnancy. Reproduction 2002; 123:379-387.
61. Machaty Z, Day BN, Prather RS. Development of early porcine embryos in vitro and in vivo. Biol Reprod 1998; 59:451-455.
62. Farin PW, Slenning BD, Britt JH. Estimates of pregnancy outcomes based on selection of bovine embryos produced in vivo or in vitro. Theriogenology 1999; 52:659-670.
63. Wang WH, Abeydeera LR, Han YM, et al. Morphologic evaluation and actin filament distribution in porcine embryos produced in vitro and in vivo. Biol Reprod 1999; 60:1020-1028.
64. Khurana NK, Niemann H. Effects of cryopreservation on glucose metabolism and survival of bovine morulae and blastocysts derived in vitro or in vivo. Theriogenology 2000; 54:313-326.
65. Montag M, Koll B, Holmes P, van der V. Significance of the number of embryonic cells and the state of the zona pellucida for hatching of mouse blastocysts in vitro versus in vivo. Biol Reprod 2000; 62:1738-1744.
66. Holm P, Booth PJ, Callesen H. Kinetics of early in vitro development of bovine in vivo- and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media. Reproduction 2002; 123:553-565.
67. Papadopoulos S, Rizos D, Duffy P, et al. Embryo survival and recipient pregnancy rates after transfer of fresh or vitrified, in vivo or in vitro produced ovine blastocysts. Anim Reprod Sci 2002; 74:35-44.
68. Rizos D, Ward F, Duffy P, et al. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Mol Reprod Dev 2002; 61:234-248.
69. Watkins AJ, Platt D, Papenbrock T, et al. Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure. Proc Natl Acad Sci U S A 2007; 104:5449-5454. A study demonstrating increased systolic blood pressure and elevated serum angiotensin-converting enzyme and hepatic phosphoenolpyruvate carboxykinase, which regulate cardiovascular and metabolic physiology, in adult mice produced through IVF.
70. Giritharan G, Talbi S, Donjacour A, et al. Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos. Reproduction 2007; 134:63-72. A study using DNA microarray technology to measure global patterns of gene expression in mouse blastocysts either conceived in vivo or produced through IVF. Statistically significant differences in expression of 1912 genes were observed in IVF versus in-vivo blastocysts. Expression of 29 genes differed by more than four-fold.
71. Boni R, Tosti E, Roviello S, Dale B. Intercellular communication in in vivo- and in vitro-produced bovine embryos. Biol Reprod 1999; 61:1050-1055.
72. Rizos D, Fair T, Papadopoulos S, et al. Developmental, qualitative, and ultrastructural differences between ovine and bovine embryos produced in vivo or in vitro. Mol Reprod Dev 2002; 62:320-327.
73. Crosier AE, Farin PW, Dykstra MJ, et al. Ultrastructural morphometry of bovine compact morulae produced in vivo or in vitro. Biol Reprod 2000; 62:1459-1465.
74. Crosier AE, Farin PW, Dykstra MJ, et al. Ultrastructural morphometry of bovine blastocysts produced in vivo or in vitro. Biol Reprod 2001; 64:1375-1385.
75. Abe H, Otoi T, Tachikawa S, et al. Fine structure of bovine morulae and blastocysts in vivo and in vitro. Anat Embryol (Berl) 1999; 199:519-527.
76. Long CR, Dobrinsky JR, Garrett WM, Johnson LA. Dual labeling of the cytoskeleton and DNA strand breaks in porcine embryos produced in vivo and in vitro. Mol Reprod Dev 1998; 51:59-65.
77. Khurana NK, Niemann H. Energy metabolism in preimplantation bovine embryos derived in vitro or in vivo. Biol Reprod 2000; 62:847-856.
78. Swain JE, Bormann CL, Clark SG, et al. Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 2002; 123:253-260.
79. Wrenzycki C, Herrmann D, Carnwath JW, Niemann H. Expression of the gap junction gene connexin43 (Cx43) in preimplantation bovine embryos derived in vitro or in vivo. J Reprod Fertil 1996; 108:17-24.
80. Eckert J, Niemann H. mRNA expression of leukaemia inhibitory factor (LIF) and its receptor subunits glycoprotein 130 and LIF-receptor-beta in bovine embryos derived in vitro or in vivo. Mol Hum Reprod 1998; 4:957-965.
81. Stojanov T, Alechna S, O'Neill C. In-vitro fertilization and culture of mouse embryos in vitro significantly retards the onset of insulin-like growth factor-II expression from the zygotic genome. Mol Hum Reprod 1999; 5:116-124.
82. Stojanov T, O'Neill C. Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro on its expression. Biol Reprod 1999; 60:674-682.
83. Stojanov T, O'Neill C. In vitro fertilization causes epigenetic modifications to the onset of gene expression from the zygotic genome in mice. Biol Reprod 2001; 64:696-705.
84. Bertolini M, Beam SW, Shim H, et al. Growth, development, and gene expression by in vivo- and in vitro-produced day 7 and 16 bovine embryos. Mol Reprod Dev 2002; 63:318-328.
85. Lazzari G, Wrenzycki C, Herrmann D, et al. Cellular and molecular deviations in bovine in vitro-produced embryos are related to the large offspring syndrome. Biol Reprod 2002; 67:767-775.
86. Buster JE, Bustillo M, Rodi IA, et al. Biologic and morphologic development of donated human ova recovered by nonsurgical uterine lavage. Am J Obstet Gynecol 1985; 153:211-217.
87. Behr B, Fisch JD, Racowsky C, et al. Blastocyst-ET and monozygotic twinning. J Assist Reprod Genet 2000; 17:349-351.
88. Milki AA, Jun SH, Hinckley MD, et al. Incidence of monozygotic twinning with blastocyst transfer compared to cleavage-stage transfer. Fertil Steril 2003; 79:503-506.
89. Wright V, Schieve LA, Vahratian A, Reynolds MA. Monozygotic twinning associated with day 5 embryo transfer in pregnancies conceived after IVF. Hum Reprod 2004; 19:1831-1836.
90. van Wagtendonk-de Leeuw AM, Aerts BJ, den Daas JH. Abnormal offspring following in vitro production of bovine preimplantation embryos: a field study. Theriogenology 1998; 49:883-894.
91. Bertolini M, Mason JB, Beam SW, et al. Morphology and morphometry of in vivo- and in vitro-produced bovine concepti from early pregnancy to term and association with high birth weights. Theriogenology 2002; 58:973-994.
92. Bertolini M, Moyer AL, Mason JB, et al. Evidence of increased substrate availability to in vitro-derived bovine foetuses and association with accelerated conceptus growth. Reproduction 2004; 128:341-354.
93. Miles JR, Farin CE, Rodriguez KF, et al. Angiogenesis and morphometry of bovine placentas in late gestation from embryos produced in vivo or in vitro. Biol Reprod 2004; 71:1919-1926.
94. Sjoblom C, Roberts CT, Wikland M, Robertson SA. Granulocyte-macrophage colony-stimulating factor alleviates adverse consequences of embryo culture on fetal growth trajectory and placental morphogenesis. Endocrinology 2005; 146:2142-2153.
95. Farin PW, Farin CE. Transfer of bovine embryos produced in vivo or in vitro: survival and fetal development. Biol Reprod 1995; 52:676-682.
96. Maxfield EK, Sinclair KD, Broadbent PJ, et al. Short-term culture of ovine embryos modifies fetal myogenesis. Am J Physiol 1998; 274:E1121-E1123.
97. Crosier AE, Farin CE, Rodriguez KF, et al. Development of skeletal muscle and expression of candidate genes in bovine fetuses from embryos produced in vivo or in vitro. Biol Reprod 2002; 67:401-408.
98. Young LE, Fernandes K, McEvoy TG, et al. Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nat Genet 2001; 27:153-154.
99. Hiendleder S, Mund C, Reichenbach HD, et al. Tissue-specific elevated genomic cytosine methylation levels are associated with an overgrowth phenotype of bovine fetuses derived by in vitro techniques. Biol Reprod 2004; 71:217-223.
100. Hiendleder S, Wirtz M, Mund C, et al. Tissue-specific effects of in vitro fertilization procedures on genomic cytosine methylation levels in overgrown and normal sized bovine fetuses. Biol Reprod 2006; 75:17-23.
101. Blondin P, Farin PW, Crosier AE, et al. In vitro production of embryos alters levels of insulin-like growth factor-II messenger ribonucleic acid in bovine fetuses 63 days after transfer. Biol Reprod 2000; 62:384-389.
102. Bertolini M, Wallace CR, Anderson GB. Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies. Reproduction 2006; 131:163-173.
103. Sangild PT, Schmidt M, Jacobsen H, et al. Blood chemistry, nutrient metabolism, and organ weights in fetal and newborn calves derived from in vitro-produced bovine embryos. Biol Reprod 2000; 62:1495-1504.
104. Rivera RM, Stein P, Weaver JR, et al. Manipulations of mouse embryos prior to implantation result in aberrant expression of imprinted genes on day 9.5 of development. Hum Mol Genet 2008; 17:1-14. A study demonstrating altered DNA methylation and misexpression of several imprinted genes during postimplantation development following IVF.
105. Moore K, Kramer JM, Rodriguez-Sallaberry CJ, et al. Insulin-like growth factor (IGF) family genes are aberrantly expressed in bovine conceptuses produced in vitro or by nuclear transfer. Theriogenology 2007; 68:717-727.
106. Jauniaux E, Englert Y, Vanesse M, et al. Pathologic features of placentas from singleton pregnancies obtained by in vitro fertilization and embryo transfer. Obstet Gynecol 1990; 76:61-64.
107. Gavriil P, Jauniaux E, Leroy F. Pathologic examination of placentas from singleton and twin pregnancies obtained after in vitro fertilization and embryo transfer. Pediatr Pathol 1993; 13:453-462.
108. Daniel Y, Schreiber L, Geva E, et al. Doplacentae of termsingleton pregnancies obtained by assisted reproductive technologies differ from those of spontaneously conceived pregnancies? Hum Reprod 1999; 14:1107-1110.
109. Lalosevic D, Tabs D, Krnojelac D, et al. Histological characteristics of placentas from assisted reproduction programs. Med Pregl 2003; 56:521-527.
110. Heinonen S, Ryynanen M, Kirkinen P, et al. Effect of in vitro fertilization on human chorionic gonadotropin serum concentrations and Down's syndrome screening. Fertil Steril 1996; 66:398-403.
111. Perheentupa A, Ruokonen A, Tuomivaara L, et al. Maternal serum beta-HCG and alpha-fetoprotein concentrations in singleton pregnancies following assisted reproduction. Hum Reprod 2002; 17:794-797.
112. Hui PW, Tang MH, Lam YH, et al. Nuchal translucency in pregnancies conceived after assisted reproduction technology. Ultrasound Obstet Gynecol 2005; 25:234-238.
113. Jacobsen H, Sangild PT, Schmidt M, et al. Macromolecule absorption and cortisol secretion in newborn calves derived from in vitro produced embryos. Anim Reprod Sci 2002; 70:1-11.
114. van Wagtendonk-de Leeuw AM, Mullaart E, de Roos AP, et al. Effects of different reproduction techniques: AI MOET or IVP, on health and welfare of bovine offspring. Theriogenology 2000; 53:575-597.
115. Numabe T, Oikawa T, Kikuchi T, Horiuchi T. Birth weight and birth rate of heavy calves conceived by transfer of in vitro or in vivo produced bovine embryos. Anim Reprod Sci 2000; 64:13-20.
116. Behboodi E, Anderson GB, BonDurant RH, et al. Birth of large calves that developed from in vitro-derived bovine embryos. Theriogenology 1995; 44:227-232.
117. McEvoy TG, Sinclair KD, Broadbent PJ, et al. Postnatal growth and development of Simmental calves derived from in vivo or in vitro embryos. Reprod Fertil Dev 1998; 10:459-464.
118. Hansen M, Kurinczuk JJ, Bower C, Webb S. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization. N Engl J Med 2002; 346:725-730.
119. Stromberg B, Dahlquist G, Ericson A, et al. Neurological sequelae in children born after in-vitro fertilisation: a population-based study. Lancet 2002; 359:461-465.
120. Helmerhorst FM, Perquin DA, Donker D, Keirse MJ. Perinatal outcome of singletons and twins after assisted conception: a systematic review of controlled studies. BMJ 2004; 328:261.
121. Jackson RA, Gibson KA, Wu YW, Croughan MS. Perinatal outcomes in singletons following in vitro fertilization: a meta-analysis. Obstet Gynecol 2004; 103:551-563.
122. Bonduelle M, Wennerholm UB, Loft A, et al. A multicentre cohort study of the physical health of 5-year-old children conceived after intracytoplasmic sperm injection, in vitro fertilization and natural conception. Hum Reprod 2005; 20:413-419.
123. Klemetti R, Gissler M, Sevon T, et al. Children born after assisted fertilization have an increased rate of major congenital anomalies. Fertil Steril 2005; 84:1300-1307.
124. Lidegaard O, Pinborg A, Andersen AN. Imprinting diseases and IVF: Danish National IVF cohort study. Hum Reprod 2005; 20:950-954.
125. Olson CK, Keppler-Noreuil KM, Romitti PA, et al. In vitro fertilization is associated with an increase in major birth defects. Fertil Steril 2005; 84:1308-1315.
126. Shevell T, Malone FD, Vidaver J, et al. Assisted reproductive technology and pregnancy outcome. Obstet Gynecol 2005; 106:1039-1045.
127. Farhi J, Fisch B. Risk of major congenital malformations associated with infertility and its treatment by extent of iatrogenic intervention. Pediatr Endocrinol Rev 2007; 4:352-357.
128. Zhu JL, Obel C, Hammer Bech B, et al. Infertility, infertility treatment, and fetal growth restriction. Obstet Gynecol 2007; 110:1326-1334.
129. Doyle P, Beral V, Maconochie N. Preterm delivery, low birthweight and small-for-gestational-age in liveborn singleton babies resulting from in-vitro fertilization. Hum Reprod 1992; 7:425-428.
130. Wang JX, Clark AM, Kirby CA, et al. The obstetric outcome of singleton pregnancies following in-vitro fertilization/gamete intra-fallopian transfer. Hum Reprod 1994; 9:141-146.
131. Koudstaal J, Braat DD, Bruinse HW, et al. Obstetric outcome of singleton pregnancies after IVF: a matched control study in four Dutch university hospitals. Hum Reprod 2000; 15:1819-1825.
132. Mahsoudi B, Li A, O'Neill C. Assessment of the long-term and transgenerational consequences of perturbing preimplantation embryo development in mice. Biol Reprod 2007; 77:889-896. A detailed transgenerational study of the developmental consequences of IVF. IVF-produced offspring exhibited slower preweaning growth rates and altered thyroid, testes and epididymal weights compared to controls. Naturally conceived offspring of IVF-produced mice also had slower preweaning growth rates and altered brain, pituitary, kidney and prostate weights compared to controls.
133. Olivennes F, Kerbrat V, Rufat P, et al. Follow-up of a cohort of 422 children aged 6 to 13 years conceived by in vitro fertilization. Fertil Steril 1997; 67:284-289.
134. Raimondi S, Pedotti P, Taioli E. Meta-analysis of cancer incidence in children born after assisted reproductive technologies. Br J Cancer 2005; 93:1053-1056.
135. Place I, Englert Y. A prospective longitudinal study of the physical, psychomotor, and intellectual development of singleton children up to 5 years who were conceived by intracytoplasmic sperm injection compared with children conceived spontaneously and by in vitro fertilization. Fertil Steril 2003; 80:1388-1397.
136. Kai CM, Main KM, Andersen AN, et al. Serum insulin-like growth factor-I (IGF-I) and growth in children born after assisted reproduction. J Clin Endocrinol Metab 2006; 91:4352-4360.
137. Miles HL, Hofman PL, Peek J, et al. In vitro fertilization improves childhood growth and metabolism. J Clin Endocrinol Metab 2007; 92:3441-3445. A study of IVF children aged 4-10 years that found significantly altered IGF expression, lipid profiles, body mass index and height compared to naturally conceived children.
138. Levy-Shiff R, Vakil E, Dimitrovsky L, et al. Medical, cognitive, emotional, and behavioral outcomes in school-age children conceived by in-vitro fertilization. J Clin Child Psychol 1998; 27:320-329.
139. Li T, Vu TH, Ulaner GA, et al. IVF results in de novo DNA methylation and histone methylation at an Igf2-H19 imprinting epigenetic switch. Mol Hum Reprod 2005; 11:631-640.
140. Fauque P, Jouannet P, Lesaffre C, et al. Assisted reproductive technology affects developmental kinetics, H19 imprinting control region methylation and H19 gene expression in individual mouse embryos. BMC Dev Biol 2007; 7:116.
141. DeBaun MR, Niemitz EL, Feinberg AP. Association of in vitro fertilization with Beckwith-Wiedemann syndrome and epigenetic alterations of LIT1 and H19. Am J Hum Genet 2003; 72:156-160.
142. Gicquel C, Gaston V, Mandelbaum J, et al. In vitro fertilization may increase the risk of Beckwith-Wiedemann syndrome related to the abnormal imprinting of the KCN1OT gene. Am J Hum Genet 2003; 72:1338-1341.
143. Maher ER, Brueton LA, Bowdin SC, et al. Beckwith-Wiedemann syndrome and assisted reproduction technology (ART). J Med Genet 2003; 40:62-64.
144. Halliday J, Oke K, Breheny S, et al. Beckwith-Wiedemann syndrome and IVF: a case-control study. Am J Hum Genet 2004; 75:526-528.
145. Sutcliffe AG, Peters CJ, Bowdin S, et al. Assisted reproductive therapies and imprinting disorders: a preliminary British survey. Hum Reprod 2006; 21:1009-1011.
146. Ludwig M, Katalinic A, Gross S, et al. Increased prevalence of imprinting defects in patients with Angelman syndrome born to subfertile couples. J Med Genet 2005; 42:289-291.
147. Doornbos ME, Maas SM, McDonnell J, et al. Infertility, assisted reproduction technologies and imprinting disturbances: a Dutch study. Hum Reprod 2007; 22:2476-2480.
148. Punjabi U, Vereecken A, Delbeke L, et al. Embryo-derived platelet activating factor, a marker of embryo quality and viability following ovarian stimulation for in vitro fertilization. J In Vitro Fert Embryo Transf 1990; 7:321-326.
149. Turpeenniemi-Hujanen T, Feinberg RF, Kauppila A, Puistola U. Extracellular matrix interactions in early human embryos: implications for normal implantation events. Fertil Steril 1995; 64:132-138.
150. Hwu YM, Chen CP, Li SH, et al. Expression of vascular endothelial growth factor messenger ribonucleic acid and protein in human preimplantation embryos. Fertil Steril 2006; 85:1830-1832.
151. O'Neill C. Evidence for the requirement of autocrine growth factors for development of mouse preimplantation embryos in vitro. Biol Reprod 1997; 56:229-237.
152. O'Neill C. Autocrine mediators are required to act on the embryo by the 2-cell stage to promote normal development and survival of mouse preimplantation embryos in vitro. Biol Reprod 1998; 58:1303-1309.
153. Jin Y, Guo XZ, Li L, et al. The effect of autocrine factors on development of early embryos of mouse. Shi Yan Sheng Wu Xue Bao 2001; 34:77-80.
154. Mansour RT, Aboulghar MA, Serour GI, Abbass AM. Co-culture of human pronucleate oocytes with their cumulus cells. Hum Reprod 1994; 9:1727-1729.
155. Freeman MR, Whitworth CM, Hill GA. Granulosa cell co-culture enhances human embryo development and pregnancy rate following in-vitro fertilization. Hum Reprod 1995; 10:408-414.
156. Quinn P, Margalit R. Beneficial effects of coculture with cumulus cells
157. Yeung WS, Lau EY, Chan ST, Ho PC. Coculture with homologous oviductal cells improved the implantation of human embryos: a prospective randomized control trial. J Assist Reprod Genet 1996; 13:762-767.
158. Kervancioglu ME, Saridogan E, Atasu T, et al. Human fallopian tube epithelial cell co-culture increases fertilization rates in male factor infertility but not in tubal or unexplained infertility. Hum Reprod 1997; 12:1253-1258.
159. Nieto FS, Watkins WB, Lopata A, et al. The effects of coculture with autologous cryopreserved endometrial cells on human in vitro fertilization and early embryo morphology: a randomized study. J Assist Reprod Genet 1996; 13:386-389.
160. Ben-Chetrit A, Jurisicova A, Casper RF. Coculture with ovarian cancer cell enhances human blastocyst formation in vitro. Fertil Steril 1996; 65:664-666.
161. Wiemer KE, Cohen J, Wiker SR, et al. Coculture of human zygotes on fetal bovine uterine fibroblasts: embryonic morphology and implantation. Fertil Steril 1989; 52:503-508.
162. Tucker MJ, Ingargiola PE, Massey JB, et al. Assisted hatching with or without bovine oviductal epithelial cell co-culture for poor prognosis in-vitro fertilization patients. Hum Reprod 1994; 9:1528-1531.
163. Hu Y, Maxson W, Hoffman D, et al. Co-culture with assisted hatching of human embryos using Buffalo rat liver cells. Hum Reprod 1998; 13:165-168.
164. Schillaci R, Ciriminna R, Cefalu E. Vero cell effect on in-vitro human blastocyst development: preliminary results. Hum Reprod 1994; 9:1131-1135.
165. Magli MC, Gianaroli L, Ferraretti AP, et al. Human embryo co-culture: results of a randomized prospective study. Int J Fertil Menopausal Stud 1995; 40:254-259.
166. Turner K, Lenton EA. The influence of Vero cell culture on human embryo development and chorionic gonadotrophin production in vitro. Hum Reprod 1996; 11:1966-1974.
167. Desai N, Goldfarb J. Co-cultured human embryos may be subjected to widely different microenvironments: pattern of growth factor/cytokine release by Vero cells during the co-culture interval. Hum Reprod 1998; 13:1600-1605.
168. Shapiro B, Richter K, Harris D, Daneshmand S. A randomized controlled study comparing human embryo growth in sequential blastocyst media with or without Vero cell coculture. Fertil Steril 2001; 76:s3-s4.
169. Desai N, Lawson J, Goldfarb J. Assessment of growth factor effects on postthaw development of cryopreserved mouse morulae to the blastocyst stage. Hum Reprod 2000; 15:410-418.
170. Lim KT, Jang G, Ko KH, et al. Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media. Theriogenology 2007; 67:293-302.
171. Desai N, Kattal N, AbdelHafez FF, et al. Granulocyte-macrophage colony stimulating factor (GM-CSF) and co-culture can affect postthaw development and apoptosis in cryopreserved embryos. J Assist Reprod Genet 2007; 24:215-222. A study comparing postthaw development of cryopreserved mouse zygotes grown in control media or media supplemented with one of 11 different growth factors or Vero cell coculture. Coculture and all but one of the individual growth factors significantly improved inner cell mass quality compared to controls. GM-CSF significantly reduced apoptosis. Blastocyst cell numbers were significantly higher with coculture than with any individual growth factor or controls.
172. Aflalo ED, Sod-Moriah UA, Potashnik G, Har-Vardi I. EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate. Reprod Biol Endocrinol 2007; 5:4. A study reporting that EGF supplementation of embryo culture media increased the expression of plasminogen activators in rat embryos and doubled their implantation rate.
173. Behr B, Mooney S, Wen Y, et al. Preliminary experience with low concentration of granulocyte-macrophage colony-stimulating factor: a potential regulator in preimplantation mouse embryo development and apoptosis. J Assist Reprod Genet 2005; 22:25-32.
174. Robertson SA, Sjoblom C, Jasper MJ, et al. Granulocyte-macrophage colony-stimulating factor promotes glucose transport and blastomere viability in murine preimplantation embryos. Biol Reprod 2001; 64:1206-1215.
175. Papayannis M, Eyheremendy V, Sanjurjo C, et al. Effect of granulocyte-macrophage colony stimulating factor on growth, resistance to freezing and thawing and re-expansion of murine blastocysts. Reprod Biomed Online 2007; 14:96-101. A study reporting that GM-CSF supplementation of embryo culture media increased blastocyst cell numbers and reexpansion rates following cryopreservation.
176. Shapiro B, Richter K, Daneshmand S, et al. Granulocyte-macrophage colony-stimulating factor enhances human embryo development to the blastocyst stage: a randomized study. Fertil Steril 2003; 79:15-16.
177. Sjoblom C, Wikland M, Robertson SA. Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro. Hum Reprod 1999; 14:3069-3076.
178. de Moraes AA, Hansen PJ. Granulocyte-macrophage colony-stimulating factor promotes development of in vitro produced bovine embryos. Biol Reprod 1997; 57:1060-1065.
179. Kim D, Kim M, Kang H, et al. The supplementation of granulocyte-macrophage colony-stimulating factor (GM-CSF) in culturemediumimproves the pregnancy rate in human ART programs. Fertil Steril 2001; 76:s6.
180. Tamada H, Higashiyama C, Takano H, et al. The effects of heparin-binding epidermal growth factor-like growth factor on preimplantation-embryo development and implantation in the rat. Life Sci 1999; 64:1967-1973.
181. Martin KL, Barlow DH, Sargent IL. Heparin-binding epidermal growth factor significantly improves human blastocyst development and hatching in serum-free medium. Hum Reprod 1998; 13:1645-1652.
182. Lim JJ, Lee DR, Song HS, et al. Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin alphanubeta3) expression in peri-implantation mouse embryos. J Assist Reprod Genet 2006; 23:111-119.
183. Kim S, Lee SH, Kim JH, et al. Antiapoptotic effect of insulin-like growth factor (IGF)-I and its receptor in porcine preimplantation embryos derived from in vitro fertilization and somatic cell nuclear transfer. Mol Reprod Dev 2006; 73:1523-1530.
184. Herrler A, Krusche CA, Beier HM. Insulin and insulin-like growth factor-I promote rabbit blastocyst development and prevent apoptosis. Biol Reprod 1998; 59:1302-1310.
185. Byrne AT, Southgate J, Brison DR, Leese HJ. Regulation of apoptosis in the bovine blastocyst by insulin and the insulin-like growth factor (IGF) superfamily. Mol Reprod Dev 2002; 62:489-495.
186. Makarevich AV, Markkula M. Apoptosis and cell proliferation potential of bovine embryos stimulated with insulin-like growth factor I during in vitro maturation and culture. Biol Reprod 2002; 66:386-392.
187. Spanos S, Becker DL, Winston RM, Hardy K. Antiapoptotic action of insulin-like growth factor-I during human preimplantation embryo development. Biol Reprod 2000; 63:1413-1420.
188. Harvey MB, Kaye PL. Insulin-like growth factor-1 stimulates growth of mouse preimplantation embryos in vitro. Mol Reprod Dev 1992; 31:195-199.
189. Lin TC, Yen JM, Gong KB, et al. IGF-1/IGFBP-1 increases blastocyst formation and total blastocyst cell number in mouse embryo culture and facilitates the establishment of a stem-cell line. BMC Cell Biol 2003; 4:14.
190. Kim S, Lee GS, Lee SH, et al. Embryotropic effect of insulin-like growth factor (IGF)-I and its receptor on development of porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer. Mol Reprod Dev 2005; 72:88-97.
191. Moreira F, Paula-Lopes FF, Hansen PJ, et al. Effects of growth hormone and insulin-like growth factor-I on development of in vitro derived bovine embryos. Theriogenology 2002; 57:895-907.
192. Sirisathien S, Hernandez-Fonseca HJ, Brackett BG. Influences of epidermal growth factor and insulin-like growth factor-I on bovine blastocyst development in vitro. Anim Reprod Sci 2003; 77:21-32.
193. Palma GA, Muller M, Brem G. Effect of insulin-like growth factor I (IGF-I) at high concentrations on blastocyst development of bovine embryos produced in vitro. J Reprod Fertil 1997; 110:347-353.
194. Block J, Drost M, Monson RL, et al. Use of insulin-like growth factor-I during embryo culture and treatment of recipients with gonadotropin-releasing hormone to increase pregnancy rates following the transfer of in vitro-produced embryos to heat-stressed, lactating cows. J Anim Sci 2003; 81:1590-1602.
195. Mitchell MH, Swanson RJ, Hodgen GD, Oehninger S. Enhancement of in vitro murine embryo development by recombinant leukemia inhibitory factor. J Soc Gynecol Investig 1994; 1:215-219.
196. Fry RC, Batt PA, Fairclough RJ, Parr RA. Human leukemia inhibitory factor improves the viability of cultured ovine embryos. Biol Reprod 1992; 46:470-474.
197. Ptak G, Lopes F, Matsukawa K, et al. Leukaemia inhibitory factor enhances sheep fertilization in vitro via an influence on the oocyte. Theriogenology 2006; 65:1891-1899.
198. Mezhevikina LM, Fedorova VV, Kapralova IV, Fesenko EE. Increased survival of preimplantation mouse embryos in medium with recombinant cytokine LIF. Ontogenez 2006; 37:55-62.
199. Dunglison GF, Barlow DH, Sargent IL. Leukaemia inhibitory factor significantly enhances the blastocyst formation rates of human embryos cultured in serum-free medium. Hum Reprod 1996; 11:191-196.
200. Lavranos TC, Rathjen PD, Seamark RF. Trophic effects of myeloid leukaemia inhibitory factor (LIF) on mouse embryos. J Reprod Fertil 1995; 105:331-338.
201. Borini A, Bulletti C, Cattoli M, et al. Use of recombinant leukemia inhibitory factor in embryo implantation. Ann N Y Acad Sci 1997; 828:157-161.
202. Bhatnagar P, Papaioannou VE, Biggers JD. CSF-1 and mouse preimplantation development in vitro. Development 1995; 121:1333-1339.
203. Roudebush WE, Duralia DR, Butler WJ. Effect of platelet-activating factor (PAF) on preimplantation mouse B6D2F1/J embryo formation. Am J Reprod Immunol 1996; 35:272-276.
204. O'Neill C, Ryan JP, Collier M, et al. Supplementation of in-vitro fertilisation culture medium with platelet activating factor. Lancet 1989; 2:769-772.
205. Roudebush WE, Massey JB, Kort HI, et al. Exposure of preimplantation embryos to platelet-activating factor increases birth rate. J Assist Reprod Genet 2004; 21:297-300.
206. Zhou P, Liu DJ, Cang M, et al. TGFalpha and EGFR in ovine preimplantation embryos and effects on development. Anim Reprod Sci 2008; 104:370-381. A study reporting that TGF-α supplementation of embryo culture media increased developmental rates and reduced apoptosis.
207. Taniguchi F, Harada T, Yoshida S, et al. Paracrine effects of bFGF and KGF on the process of mouse blastocyst implantation. Mol Reprod Dev 1998; 50:54-62.
208. Shi H, Miller F, Miller K, Kim MH. The effect of platelet activating factor on different phases of murine in vitro fertilization. J Assist Reprod Genet 1992; 9:373-377.
209. Khamsi F, Armstrong DT, Zhang X. Expression of urokinase-type plasminogen activator in human preimplantation embryos. Mol Hum Reprod 1996; 2:273-276.
210. Huang JX, Mehrens D, Wiese R, et al. High-throughput genomic and proteomic analysis using microarray technology. Clin Chem 2001; 47:1912-1916.
211. Soulet D, Rivest S. Perspective: how to make microarray, serial analysis of gene expression, and proteomic relevant to day-to-day endocrine problems and physiological systems. Endocrinology 2002; 143:1995-2001.
212. Angelucci S, Ciavardelli D, Di Giuseppe F, et al. Proteome analysis of human follicular fluid. Biochim Biophys Acta 2006; 1764:1775-1785.
213. Shaw JL, Smith CR, Diamandis EP. Proteomic analysis of human cervicovaginal fluid. J Proteome Res 2007; 6:2859-2865.
214. Hanrieder J, Nyakas A, Naessen T, Bergquist J. Proteomic analysis of human follicular fluid using an alternative bottom-up approach. J Proteome Res 2008; 7:443-449. A detailed proteomic analysis of follicular fluid that identified 69 proteins, including 32 not previously reported in proteomic studies of follicular fluid.
215. Richter KS, Shipley SK, McVearry I, et al. Cryopreserved embryo transfers suggest that endometrial receptivity may contribute to reduced success rates of later developing embryos. Fertil Steril 2006; 86:862-866.
216. Shapiro BS, Daneshmand ST, Garner FC, et al. Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor, and cryopreserved cycles with the use of day 5 or day 6 blastocysts may reflect differences in embryo-endometrium synchrony. Fertil Steril 2008; 89:20-26.
217. Magli MC, Jones GM, Gras L, et al. Chromosome mosaicism in day 3 aneuploid embryos that develop to morphologically normal blastocysts in vitro. Hum Reprod 2000; 15:1781-1786.
218. Sandalinas M, Sadowy S, Alikani M, et al. Developmental ability of chromosomally abnormal human embryos to develop to the blastocyst stage. Hum Reprod 2001; 16:1954-1958.
219. Rubio C, Rodrigo L, Mercader A, et al. Impact of chromosomal abnormalities on preimplantation embryo development. Prenat Diagn 2007; 27:748-756.
220. Menezo YJ, Sakkas D. Monozygotic twinning: is it related to apoptosis in the embryo? Hum Reprod 2002; 17:247-248.