Clinical assisted reproduction: Beneficial effects of coculture with cumulus cells on blastocyst formation in a prospective trial with supernumerary human embryos

Journal of Assisted Reproduction and Genetics

Volumn 13, Issue 1, 1996, Pages 9-14

  Quinn, Patrick ^a,b   Margalit, Ruth ^a  

^a San Fernando Valley Women's Center   (United States)

^b NONE   (United States)

Author keywords

Blastocyst; Coculture; Cumulus cell; Extended culture

Indexed keywords

ARTICLE; ARTIFICIAL INSEMINATION; BLASTOCYST; CLINICAL PROTOCOL; CULTURE MEDIUM; CUMULUS OOPHORUS; EMBRYO; EMBRYO CULTURE; EMBRYO TRANSFER; FEMALE; FERTILIZATION IN VITRO; HUMAN; INFERTILITY; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; PROSPECTIVE STUDY;

BLASTOCYST; CELL SURVIVAL; COCULTURE TECHNIQUES; CRYOPRESERVATION; CULTURE MEDIA; FEMALE; FERTILIZATION IN VITRO; FIBRONECTINS; HUMANS; MALE; SPERMATOZOA;

EID: 0030041229     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02068862     Document Type: Article

References (20)

1. Quinn P: Use of coculture with cumulus cells in insemination medium in human in vitro fertilization (IVF). J Assist Reprod Genet 1994;11:270-277
2. Liu J, Lissens W, Devroey P, Van Steirteghem A, Liebaers I: Polymerase chain reaction analysis of the cystic fibrosis F508 mutation in human blastomeres following oocyte injection of a single sperm from a carrier. Prenatal Diagn 1993;13:873-880
3. Pickering SJ, Muggleton-Harris AL: Reliability and accuracy of polymerase chain reaction amplification of two unique target sequences from biopsies of cleavage-stage and blastocyst-stage human embryos. Hum Reprod 1995;10:1021-1029
4. Wiemer KE, Cohen J, Wiker SR, Malter HE, Wright G, Godke RA: Coculture of human zygotes on fetal bovine uterine fibroblasts; embryonic morphology and implantation. Fertil Steril 1989;52:503-508
5. Bongso A, Ng S-C, Sathananthan H, Lian NP, Rauff M, Ratnam S: Improved quality of human embryos when co-cultured with human ampullary cells. Hum Reprod 1989;4:706-713
6. Thibodeaux JK, Godke RA: In vitro enhancement of early-stage embryos with co-culture. Arch Pathol Lab Med 1992;116:364-372
7. Bavister BD: Co-culture for embryo development: is it really necessary? Hum Reprod 1992;7:1339-1341
8. Wiemer KE, Hoffman DI, Maxson WS, Eager S, Muhlberger B, Fiore I, Cuervo M: Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells. Hum Reprod 1993;8:97-101
9. Bongso A, Fong C-Y, Ng S-C, Ratnam S: Human embryonic behavior in a sequential human oviduct-endometrial coculture system. Fertil Steril 1994;61:976-978
10. Menezo YJR, Guerin J-F, Czyba J-C: Improvement of human early embryo development in vitro by coculture on monolayers of Vero cells. Biol Reprod 1990;42:301-306
11. Plachot M, Antoine JM, Alvarez S, Firmin C, Pfister A, Mandelbaum J, Junca A-M, Salat-Baroux J: Granulosa cells improve human embryo development in vitro. Hum Reprod 1993;8:2133-2140
12. Freeman MR, Whitworth CM, Hill GA: Granulosa cell co-culture enhances human embryo development and pregnancy rate following in-vitro fertilization. Hum Reprod 1995;10:408-414
13. Mansour RT, Aboulghar MA, Serour GI, Abbass AM: Co-culture of human pronucleate oocytes with their cumulus cells. Hum Reprod 1994;9:1727-1729
14. Saito H, Hirayama T, Koike K, Saito T, Nohara M, Hiroi M: Cumulus mass maintains embryo quality. Fertil Steril 1994;62:555-558
15. Goto K, Kajihara Y, Kosaka S, Koba M, Nakanishi Y, Ogawa K: Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes. J Reprod Fert 1988;83:753-758
16. Fukuda Y, Ichikawa M, Naito K Toyoda Y: Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to the blastocyst stage. Biol Reprod 1990;42:114-119
17. Quinn P, Margalit R: Significantly greater blastocyst formation with cumulus cell co-culture in a prospective trial with supernumerary human embryos. In IXth World Congress on In Vitro Fertilization and Assisted Reproduction, A Aburumieh, E Bernat, G Dohr, W Feichtinger, F Fischl, J Huber, E Muller, S Szalay, W Urdl, H Zech (eds). Bologna, Monduzzi Editore, 1995, pp 487-491
18. Van Blerkom J: Development of human embryos to the hatched blastocyst stage in the presence or absence of a monolayer of Vero cells. Hum Reprod 1993;8:1525-1539
19. Sakkas D, Jaquenoud N, Leppens G, Campana A: Comparison of results after in vitro fertilized human embryos are cultured in routine medium and in coculture on Vero cells: A randomized study. Fertil Steril 1994;61:521-525
20. Lopata A: The neglected human blastocyst. J Assist Reprod Genet 1992;9:508-512