The use of Endo-Porter to deliver morpholinos in kidney organ culture

BioTechniques

Volumn 44, Issue 4, 2008, Pages 547-549

  Nikopoulos, George N ^a,b   Adams, Tamara L ^a   Adams, Derek ^a   Oxburgh, Leif ^a   Prudovsky, Igor ^a   Verdi, Joseph M ^a  

^a Maine Medical Center Research Institute   (United States)

^b UNIVERSITY OF MAINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MORPHOLINE DERIVATIVE;

CELL INTERACTION; DEVICE; ENDO PORTER; GENE DISRUPTION; IN VIVO STUDY; KIDNEY CELL CULTURE; NONHUMAN; ORGAN CULTURE; PROTEIN EXPRESSION; SHORT SURVEY; WESTERN BLOTTING;

ANIMALS; DRUG DELIVERY SYSTEMS; FLUORESCEIN; FLUORESCENT DYES; KIDNEY; MICE; MICE, INBRED ICR; MODELS, BIOLOGICAL; NEOPLASM PROTEINS; OLIGONUCLEOTIDES, ANTISENSE; ORGAN CULTURE TECHNIQUES; PEPTIDES; RNA, MESSENGER; TIME FACTORS;

MUS;

EID: 43149086012     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112725     Document Type: Short Survey

References (15)

1. MAPK acts in the BMP7-dependent stimulatory pathway during epithelial cell morphogenesis and is regulated by Smad1. J. Biol. Chem. 279:12051-12059.
2. al-Awqati, Q. and M.R. Goldberg. 1998. Architectural patterns in branching morphogenesis in the kidney. Kidney Int. 54:1832-1842.
3. Piscione, T.D. and N.D. Rosenblum. 2002. The molecular control of renal branching morphogenesis: current knowledge and emerging insights. Differentiation 70:227-246.
4. Davies, J.A., M. Ladomery, P. Hohenstein, L. Michael, A. Shafe, L. Spraggon, and N. Hastie. 2004. Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation. Hum. Mol. Genet. 13:235-246.
5. Summerton, J., D. Stein, S.B. Huang, P. Matthews, D. Weller, and M. Partridge. 1997. Morpholino and phosphorothioate antisense oligomers compared in cell-free and in-cell systems. Antisense Nucleic Acid Drug Dev. 7:63-70.
6. Kendall, S.E., C. Battelli, S. Irwin, J.G. Mitchell, C.A. Glackin, and J.M. Verdi. 2005. NRAGE mediates p38 activation and neural progenitor apoptosis via the bone morphogenetic protein signaling cascade. Mol. Cell. Biol. 25:7711-7724.
7. Salehi, A.H., P.P. Roux, C.J. Kubu, C. Zeindler, A. Bhakar, L.L. Tannis, J.M. Verdi, and P.A. Barker. 2000. NRAGE, a novel MAGE protein, interacts with the p75 neurotrophin receptor and facilitates nerve growth factor-dependent apoptosis. Neuron 27:279-288.
8. Summerton, J.E. 2005. Endo-Porter: a novel reagent for safe, effective delivery of substances into cells. Ann. N.Y. Acad. Sci. 1058:62-75.
9. Grobstein, C. 1953. Morphogenetic interaction between embryonic mouse tissues separated by a membrane filter. Nature 172:869-870.
10. Avner, E.D., D. Ellis, T. Temple, and R. Jaffe. 1982. Metanephric development in serum-free organ culture. In Vitro 18:675-682.
11. Kanwar, Y.S., K. Ota, Q. Yang, J. Wada, N. Kashihara, Y. Tian, and E.I. Wallner. 1999. Role of membrane-type matrix metalloproteinase 1 (MT-1-MMP), MMP-2, and its inhibitor in nephrogenesis. Am. J. Physiol. 277:F934-F947.
12. Liu, Z.Z., J. Wada, A. Kumar, F.A. Carone, M. Takahashi, and Y.S. Kanwar. 1996. Comparative role of phosphotyrosine kinase domains of c-ros and c-ret protooncogenes in metanephric development with respect to growth factors and matrix morphogens. Dev. Biol. 178:133-148.
13. Quaggin, S.E., G.B. Vanden Heuvel, and P. Igarashi. 1998. Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney. Mech. Dev. 71:37-48.
14. Gross, I., D.J. Morrison, D.P. Hyink, K. Georgas, M.A. English, M. Mericskay, S. Hosono, D. Sassoon, et al. 2003. The receptor tyrosine kinase regulator Sprouty1 is a target of the tumor suppressor WT1 and important for kidney development. J. Biol. Chem. 278:41420-41430.
15. Srinivas, S., M.R. Goldberg, T. Watanabe, V. D'Agati, Q. al-Awqati, and F. Costantini. 1999. Expression of green fluorescent protein in the ureteric bud of transgenic mice: a new tool for the analysis of ureteric bud morphogenesis. Dev. Genet. 24:241-251.