Expression, refolding, and purification of a truncated human Delta-like1, a ligand of Notch receptors

Protein Expression and Purification

Volumn 59, Issue 2, 2008, Pages 242-248

  Shi, Zhan Xia ^a   He, Fei ^b   Wang, Li Li ^a   Liang, Ying Min ^c   Han, Hua ^b,c   Wang, Chao Zhan ^a   Zhao, Qun ^a   Geng, Xin Du ^a  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^c FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Chromatography; Human Delta like1; Prokaryotic expression; Purification; Refolding

Indexed keywords

DLK1 PROTEIN, HUMAN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; LIGAND; MEMBRANE PROTEIN; NOTCH RECEPTOR; SIGNAL PEPTIDE;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; PROTEIN FOLDING; REPORTER GENE;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; GENES, REPORTER; GLUTATHIONE TRANSFERASE; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LIGANDS; MASS SPECTROMETRY; MEMBRANE PROTEINS; PROTEIN FOLDING; RECEPTORS, NOTCH; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI; PROKARYOTA;

EID: 43049159740     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.02.004     Document Type: Article

References (31)

1. Artavanis-Tsakonas S., Matsuno K., and Fortini M.E. Notch signaling. Science 268 (1995) 225-232
2. Swiatek P.J., Lindsell C.E., del Amo F.F., Weinmaster G., and Gridley T. Notch1 is essential for postimplantation development in mice. Genes Dev. 8 (1994) 707-719
3. Conlon R.A., Reaume A.G., and Rossant J. Notch1 is required for the coordinate segmentation of somites. Development 121 (1995) 1533-1545
4. Oka C., Nakano T., Wakeham A., de la Pompa J.L., Mori C., Sakai T., Kawaichi M., Shiota K., Mak T.M., and Honjo T. Disruption of the mouse RBP-J kappa gene results in early embryonic death. Development 121 (1995) 3291-3301
5. Radtke F., Wilson A., Stark G., Bauer M., van Meerwijk J., MacDonald H.R., and Aguet M. Deficient T cell fate specification in mice with an induced inactivation of Notch1. Immunity 10 (1999) 547-558
6. Han H., Tanigaki K., Yamamoto N., Kuroda K., Yoshimoto M., Nakahata T., Ikuta K., and Honjo T. RBP-J deficient mouse reveals essential role of Notch in T versus B lineage determination but not in later stages of thymocyte development. Int. Immunol. 14 (2002) 637-645
7. Joutel A., Corpechot C., Ducros A., Vahedi K., Chabriat H., Mouton P., Alamowitch S., Domenga V., Cecillion M., Marechal E., Maciazek J., Vayssiere C., Cruaud C., Cabanis E.A., Ruchoux M.M., Weissenbach J., Bach J.F., Bousser M.G., and Tournier-Lasserve E. Notch3 mutations in CADASIL, a hereditary adult-onset condition causing stroke and dementia. Nature 383 (1996) 707-710
8. Joutel A., and Tournier-Lasserve E. Notch signaling pathway and human diseases. Semin. Cell Dev. Biol. 9 (1998) 619-625
9. Roy M., Pear W.S., and Aster J.C. The multifaceted role of Notch in cancer. Curr. Opin. Genet. Dev. 17 (2007) 52-59
10. Thurston G., Noguera-Troise I., and Yancopoulos G.D. The Delta paradox: DLL4 blockade leads to more tumor vessels but less tumour growth. Nat. Rev. Cancer 7 (2007) 327-331
11. Rehman A.O., and Wang C.Y. Notch signaling in the regulation of tumor angiogenesis. Trends Cell Biol. 16 (2006) 293-300
12. Varnum-Finney B., Brashem-Stein C., and Bernstein I.D. Combined effects of Notch signaling and cytokines induce a multiple log increase in precursors with lymphoid and myeloid reconstituting ability. Blood 101 (2003) 1784-1789
13. - cord blood cells. J. Clin. Invest. 110 (2002) 1165-1174
14. Han W., Ye Q., and Moore M.A.S. A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells. Blood 95 (2000) 1616-1625
15. Swietnicki W. Folding aggregated proteins into functionally active forms. Curr. Opin. Biotechnol. 17 (2006) 367-372
16. Tsumoto K., Ejima D., Kumagai I., and Arakawa T. Practical considerations in refolding proteins from inclusion bodies. Protein Expr. Purif. 28 (2003) 1-8
17. Gu Z.Y., Weidenhaupt M., Ivanova N., Pavlov M., Xu B.Z., Su Z.G., and Janson J.C. Chromatographic methods for the isolation of and refolding of proteins from Escherichia coli inclusion bodies. Protein Expr. Purif. 25 (2002) 174-179
18. Geng X.D., and Wang C.Z. Protein folding liquid chromatography and its recent developments. J. Chromatogr. B 849 (2007) 69-80
19. Wang C.Z., Wang L.L., and Geng X.D. Refolding and purification of recombinant human granulocyte colony-stimulating factor expressed in Escherichia coli using protein folding liquid chromatography. Chin. J. Chromatogr. 25 (2007) 514-517
20. Geng X.D., Bai Q., Zhang Y.J., Li X., and Wu D. Refolding and purification of interferon-gamma in industry by hydrophobic interaction chromatography. J. Biotechnol. 113 (2004) 137-149
21. Wang L.L., Wang C.Z., and Geng X.D. Refolding with simultaneous purification of recombinant human stem cell factor expressed in Escherichia coli by high performance hydrophobic interaction chromatography. Biotechnol. Lett. 28 (2006) 993-997
22. Lichty J.J., Malecki J.L., Agnew H.D., Michelson-Horowitz D.J., and Tan S. Comparison of affinity tags for protein purification. Protein Expr. Purif. 41 (2005) 98-105
23. Azarkan M., Huet J., Baeyens-Volant D., Looze Y., and Vandenbussche G. Affinity chromatography: a useful tool in proteomics studies. J. Chromatogr. B 849 (2007) 81-90
24. Wang C.Z., Wang L.L., and Geng X.D. Renaturation of recombinant human granulocyte colony-stimulating factor produced from Escherichia coli using size exclusion chromatography. J. Liquid Chromatogr. Related Tech. 29 (2006) 203-217
25. Qin H., Wang J., Liang Y., Taniguchi Y., Tanigaki K., and Han H. RING1 inhibits transactivation of RBP-J by Notch though interaction with LIM protein KyoT2. Nucleic Acids Res. 32 (2004) 1492-1501
26. Berkner H., Engelhorn J., Liscombe D.K., Schweimer K., Wöhrl B.M., Facchini P.J., Rösch P., and Matečko I. High-yield expression and purification of isotopically labeled norcoclaurine synthase, a Bet v 1-homologous enzyme, from Thalictrum flavum for NMR studies. Protein Expr. Purif. 56 (2007) 197-204
27. Li M.G., and Huang D.Y. On-column refolding purification and characterization of recombinant human interferon-λ1 produced in Escherichia coli. Protein Expr. Purif. 53 (2007) 119-123
28. Harrowing S.R., and Chaudhuri J.B. Effect of column dimensions and flow rates on size-exclusion refolding of beta-lactamase. J. Biochem. Biophys. Methods 56 (2003) 177-188
29. Gu Z., Zhu X., Ni S., Zhou H., and Su Z. Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B. J. Biochem. Biophys. Methods 56 (2003) 165-175
30. Wu D., Wang C.Z., and Geng X.D. An approach for increasing the mass recovery of proteins derived from inclusion bodies in biotechnology. Biotechnol. Progr. 23 (2007) 407-413
31. Gu Z., Su Z., and Janson J.C. Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding. J. Chromatogr. A 918 (2001) 311-318