Inhibition of aggregation by media selection, sample loading and elution in size exclusion chromatographic refolding of denatured bovine carbonic anhydrase B

Journal of Biochemical and Biophysical Methods

Volumn 56, Issue 1-3, 2003, Pages 165-175

  Gu, Zhenyu ^a   Zhu, Xiaonan ^b   Ni, Shaowei ^a   Zhou, Haimeng ^a   Su, Zhiguo ^b  

^a TSINGHUA UNIVERSITY   (China)

^b INSTITUTE OF PROCESS ENGINEERING   (China)

Author keywords

Aggregation; Bovine carbonic anhydrase B; Refolding; Resolution; Size exclusion chromatography

Indexed keywords

CARBONATE DEHYDRATASE I;

ARTICLE; CATTLE; CONTROLLED STUDY; ELUTION; FLOW RATE; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; SAMPLE;

BOVINAE;

EID: 0037756778     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(03)00057-5     Document Type: Article

References (19)

1. Anfinson C. Principles that govern the folding of protein chains. Science. 181:1973;223-227.
2. Rudolph R., Lilie H. In vitro folding of inclusion body proteins. FASEB J. 10:1996;49-56.
3. Rogl H., Kosemund K., Kuhlbrandt W., Collinson I. Refolding of Escherichia coli produced membrane protein inclusion bodies immobilized by nickel chelating chromatography. FEBS Lett. 432:1998;21-26.
4. Geng X., Chang X. High-performance hydrophobic interaction chromatography as a tool for protein refolding. J. Chromatogr. 599:1992;185-194.
5. Yoshimoto M., Shimanouchi T., Umakoshi H., Kuboi R. Immobilized liposome chromatography for refolding and purification of protein. J. Chromatogr., B. 742:2000;93-99.
6. Altamirano M.M., Garcia C., Possani L.D., Fersht A.R. Oxidative refolding chromatography: folding of the scorpion toxin Cn5. Nat. Biotechnol. 17:1999;187-191.
7. Righetti P.G., Verzola B. Folding/unfolding/refolding of proteins: present methodologies in comparison with capillary zone electrophoresis. Electrophoresis. 22:2001;2359-2374.
8. Shalongo W., Ledger R., Jagannadham M.V., Stellwagen E. Refolding of denatured thioredoxin observed by size-exclusion chromatography. Biochemistry. 26:1987;3135-3141.
9. Shalongo W., Jagannadham M.V., Flynn C., Stellwagen E. Refolding of denatured ribonuclease observed by size-exclusion chromatography. Biochemistry. 28:1989;4820-4825.
10. Werner M.H., Clore G.M., Gronenborn A.M., Kondoh A., Fisher R.J. Refolding proteins by gel filtration chromatography. FEBS Lett. 345:1994;125-130.
11. Gu Z.Y., Su Z.G., Janson J.C. Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding. J. Chromatogr., A. 918:2001;311-318.
12. Batas B., Chaudhri J. Protein refolding at high concentration using size-exclusion chromatography. Biotechnol. Bioeng. 50:1996;16-23.
13. Muller C., Rinas U. Renaturation of heterodimeric platelet-derived growth factor from inclusion bodies of recombinant Escherichia coli using size-exclusion chromatography. J. Chromatogr., A. 855:1999;203-213.
14. Fahey E.M., Chaudhrui J.B., Binding P. Refolding and purification of a urokinase plasminogen activator fragment by chromatography. J. Chromatogr., B. 737:2000;225-235.
15. Ikai A.I., Tanaka S., Noda H. Reactivation kinetics of guanidine denatured bovine carbonic anhydrase B. Arch. Biochem. Biophys. 190:1978;39-45.
16. Wong K.P., Tanford C. Denaturation of bovine carbonic anhydrase B by guanidine hydrochloride. J. Biol. Chem. 248:1972;8518-8523.
17. Cleland J.L., Wang D.I.C. Refolding and aggregation of bovine carbonic anhydrase B: quasi-elastic light scattering analysis. Biochemistry. 29:1990;11072-11078.
18. Clark E.D.B., Schwarz E., Rudolph R. Inhibition of aggregation side reactions during in vitro protein folding. Methods Enzymol. 309:1999;217-236.
19. Rudolph R., Zettlmeissl G., Jaeinicke R. Reconstitution of lactic dehydrogenase. Noncovalent aggregation vs. reactivation: 1. Physical properties and kinetics of aggregation. Biochemistry. 18:1979;5572-5575.