Molecular characterization of Vibrio cholerae ΔrelA ΔspoT double mutants

Archives of Microbiology

Volumn 189, Issue 3, 2008, Pages 227-238

  Das, Bhabatosh ^a   Bhadra, Rupak K ^a  

^a INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

Author keywords

(p)ppGpp; relA; spoT; Stringent response; Vibrio cholerae

Indexed keywords

AMITROLE; BACTERIAL PROTEIN; CARBON; GLUCOSE; PROTEIN SPOT; RIBOSOME RNA; TRANSCRIPTION FACTOR RELA;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIUM MUTANT; BIOSYNTHESIS; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE CONTROL; GENE FUNCTION; MOLECULAR BIOLOGY; NONHUMAN; NUTRIENT; PHENOTYPE; PRIORITY JOURNAL; PROTEIN MALNUTRITION; VIBRIO CHOLERAE;

AMINO ACIDS; BACTERIAL PROTEINS; CARBON; GENE DELETION; GENES, ESSENTIAL; GENETIC COMPLEMENTATION TEST; GLUCOSE; GUANOSINE PENTAPHOSPHATE; GUANOSINE TETRAPHOSPHATE; LIGASES; PYROPHOSPHATASES; VIBRIO CHOLERAE;

ESCHERICHIA COLI; VIBRIO CHOLERAE;

EID: 43049091911     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-007-0312-z     Document Type: Article

References (32)

1. Arvind L, Koonin EV (1998) The HD domain defines a new superfamily of metal-dependent phosphohydrolases. Trends Biochem Sci 23:469-472
2. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1989) Current protocols in molecular biology. Wiley, New York
3. Barker MM, Gaal T, Gourse RL (2001) Mechanism of regulation of transcription initiation by ppGpp. II. Models for positive control based on properties of RNAP mutants and competition for RNAP. J Mol Biol 305:689-702
4. Braeken K, Moris M, Daniels R, Vanderleyden J, Michiels J (2006) New horizons for (p)ppGpp in bacterial and plant physiology. Trends Microbiol 14:45-54
5. Bremer H, Dennis PP (1987) Escherichia coli and Salmonella typhimurium: cellular and molecular biology, In: Neidhardt FC (ed) American Society for Microbiology, Washington, pp 1527-1542
6. Cashel M (1969) The control of ribonucleic acid synthesis in Escherichia coli. J Biol Chem 244:3133-3141
7. Cashel M, Gentry DR, Hernandes VJ, Vinella D (1996) The stringent response, In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington, pp. 1458-1496
8. Chatterji D, Ojha AK (2001) Revisiting the stringent response, ppGpp and starvation signaling. Curr Opin Microbiol 4:160-165
9. Foti JJ, Persky NS, Ferullo DJ, Lovett ST (2007) Chromosome segregation control by Escherichia coli ObgE GTPase. Mol Microbiol 65:569-581
10. Fujita C, Maeda M, Fujii T, Iwamoto R, Ikehara K (2002) Identification of an indispensable amino acid for ppGpp synthesis of Escherichia coli SpoT protein. Biosci Biotechnol Biochem 66:2735-2738
11. Gentry DR, Cashel M (1996) Mutational analysis of the Escherechia coli spoT gene identifies distinct but overlapping regions involved in ppGpp synthesis and degradation. Mol Microbiol 19:1373-1384
12. Gropp M, Strausz Y, Gross M, Glaser G (2001) Regulation of Escherichia coli RelA requires oligomerization of the C-terminal domain. J Bacteriol 183:570-579
13. Haralalka S, Nandi S, Bhadra RK (2003) Mutation in the relA gene of Vibrio cholerae affects in vitro and in vivo expression of virulence factors. J Bacteriol 185:4672-4682
14. Heidelberg JF, Eisen JA, Nelson WC, Clayton RA, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Umayam L, Gill SR, Nelson KE, Read TD, Tettelin H, Richardson D, Ermolaeva MD, Vamathevan J, Bass S, Qin H, Dragoi I, Sellers P, McDonald L, Utterback T, Fleishmann RD, Nierman WC, White O, Salzberg SL, Smith HO, Colwell RR, Mekalanos JJ, Venter JC, Fraser CM (2000) DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406:477-483
15. Hogg T, Mechold U, Malke H, Cashel M, Hilgenfeld R, (2004) Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response. Cell 117:57-68
16. Jiang M, Sullivan SM, Wout PK, Maddock JR (2007) G-protein control of the ribosome-associated stress response protein SpoT. J Bacteriol 189:6140-6147
17. Josaitis CA, Gaal T, Gourse RL (1995) Stringent control and growth-rate-dependent control have nonidentical promoter sequence requirements. Proc Natl Acad Sci USA 92:1117-1121
18. Maiti D, Das B, Saha A, Nandi RK, Nair GB, Bhadra RK (2006) Genetic organization of pre-CTX and CTX prophages in the genome of an environmental Vibrio cholerae non-O1, non-O139 strain. Microbiology 152:3633-3641
19. Mechold U, Malke H (1997) Characterization of the stringent and relaxed responses of Streptococcus equisimilis. J Bacteriol 179:2658-2667
20. Mittenhuber G (2001) Comparative genomics and evolution of genes encoding bacterial (p)ppGpp synthetases/hydrolases (the Rel, RelA and SpoT proteins). J Mol Microbiol Biotechnol 3:585-600
21. Murray K, Bremer H (1996) Control of spoT-dependent ppGpp synthesis and degradation in Escherichia coli. J Mol Biol 259:41-57
22. Nandi S, Maiti D, Saha A, Bhadra RK (2003) Genesis of variants of Vibrio cholerae O1 biotype El Tor: role of CTXΦ array and its position in the genome. Microbiology 149:89-97
23. Occhino DA, Wyckoff EE, Henderson DP, Wrona TJ, Payne SM (1998) Vibrio cholerae iron transport: haem transport genes are linked to one of two sets of tonB, exbB, exbD genes. Mol Microbiol 29:1493-1507
24. Ostling J, Flardh K, Kjelleberg S (1995) Isolation of a carbon starvation regulatory mutant in marine Vibrio strain. J Bacteriol 177:6978-6982
25. Raskin DM, Judson N, Mekalanos JJ (2007) Regulation of the stringent response is the essential function of the conserved bacterial G protein CgtA in Vibrio cholerae. Proc Natl Acad Sci USA 104:4636-4641
26. Rudd KE, Bochner BR, Cashel M, Roth JR (1985) Mutation in the spoT gene of Salmonella typhimurium: effects on his operon expression. J Bacteriol 163:534-542
27. Saha A, Haralalka S, Bhadra RK (2004) A naturally occurring point mutation in the 13-mer R repeat affects the oriC function of the large chromosome of Vibrio cholerae O1 classical biotype. Arch Microbiol 182:421-427
28. Schreiber G, Metzger S, Aizenman E, Roza S, Cashel M, Glaser G (1991) Overexpression of the relA gene in Escherichia coli. J Biol Chem 266:3760-3767
29. Silva AJ, Benitez BA (2006) A Vibrio cholerae relaxed (relA) mutant expresses major virulence factors, exhibits biofilm formation and motility, and colonizes the suckling mouse intestine. J Bacteriol 188:794-800
30. Skorupski K, Taylor RK (1996) Positive selection vectors for allelic exchange. Gene 169:47-52
31. Uzan M, Danchin A (1976) A rapid test for the relA mutation in E. coli. Biochem Biophys Res commun 69:751:758
32. Xiao H, Kalman M, Ikehara K, Zamel S, Glaser G, Cashel M, (1991) Residual guanosine 3′, 5′-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoT null mutations. J Biol Chem 266:5980