High-grade loss of leukocytes and hematopoietic progenitor cells caused by erythrocyte-lysing procedures for flow cytometric analyses

Journal of Hematotherapy and Stem Cell Research

Volumn 12, Issue 3, 2003, Pages 321-330

  Greve, Burkhard ^a   Beller, Constanze ^a   Cassens, Uwe ^a   Sibrowski, Walter ^a   Severin, Erhard ^a   Göhde, Wolfgang ^a  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CD45 ANTIGEN; FLUORESCENT DYE; REAGENT;

ANALYTICAL EQUIPMENT; ARTICLE; BLOOD SAMPLING; CELL MEMBRANE; CELL NUCLEUS; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL; HEMOLYSIS; HUMAN; HUMAN CELL; LEUKOCYTE; LEUKOCYTE COUNT; PARTICLE ANALYZING SYSTEM; PRIORITY JOURNAL; STAINING;

ANTIGENS, CD; ANTIGENS, CD34; CELL COUNT; FLOW CYTOMETRY; HEMATOLOGY; HEMATOPOIETIC STEM CELLS; HEMOLYSIS; HUMANS; LEUKOCYTES;

EID: 0037972472     PISSN: 15258165     EISSN: None     Source Type: Journal    
DOI: 10.1089/152581603322023052     Document Type: Article

References (29)

1. + T-cell counts on 'single-platform' flow cytometers. Primary CD4 gating. Br J Haematol 111:1198-1208.
2. Reimann KA, MRG O'Gormann, J Spritzler, CL Wilkening, DE Sabath, K Helm, DE Campbell and The Niaid Daids New Technologies Evaluation Group. (2000). Multisite comparison of CD4 and CD8 T-lymphocyte counting by single- versus multiple-platform methodologies: evaluation of Beckman Coulter Flow-Count Fluorospheres and the tetraONE System. Clin Diagn Lab Immunol 7:344-351.
3. + lymphocyte count across laboratories: an evaluation of the Ortho Cytoron Absolute Flow Cytometry System on normal donors. Cytometry 22:200-210.
4. + hematopoietic stem cells in the peripheral blood of high-dose cyclophospharmide-treated patients: enhancement by intravenous recombinant human granulocyte-macrophage colony-stimulating factor. Blood 74:1905-1914.
5. Civin CI, LC Strauss, C Brovall, MJ Fackler, JF Schwartz and JH Shaper. (1984). Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG1a cell. J Immunol 133:157-165.
6. + hematopoietic progenitor cell enumeration in a multicenter study. Cytometry 30:109-117.
7. + cells by flow cytometry. J Hematother 6:303-308.
8. + enumeration. Transfus Med 10:67-75.
9. + hematopoietic progenitor cells for clinical transplantation: comparison of three different methods. Bone Marrow Transplant 24:1019-1027.
10. Baech J and HE Johnsen. (2000). Technical aspects and clinical impact of hematopoietic progenitor subset quantification. Stem Cells 18:76-86.
11. Siena S, M Bregni, B Brando, N Belli, F Ravagnani, L Gandola, AC Stern, PM Lansdorp, G Bonadonna and AM Gianni. (1991). Flow cytometry for clinical estimation of circulating hematopoietic progenitors for autologous transplantation in cancer patients. Blood 77:400-409.
12. Schwartzberg L, R Birch, R Blanco, F Wittlin, J Muscato, K Tauer, B Hazelton and W West. (1993). Rapid and sustained hematopoietic reconstitution by peripheral blood stem cell infusion alone following high-dose chemotherapy. Bone Marrow Transplant 11:369-374.
13. + cell counts based on the ISHAGE guidelines. Cytometry 34:61-70.
14. + cells be analysed? A study of three classes of antibody and five leukocyte preparation procedures. J Immunol Methods 26:175-188.
15. + cells in umbilical cord blood transplants. Transfus Med 8:111-118.
16. + cells by flow cytometry: Application to the harvesting and transplantation of peripheral blood stem cells. In: Hematopoietic Stem Cells. E Wunder, H Sovalat, P Henon and S Serke, eds. AlphaMed Press, Dayton, pp 31-43.
17. Kreissig C, A Kirsch and S Serke. (1994). Characterization and measurement of CD34-expressing hematopoietic cells. J Hematother 3:263-289.
18. Bossuyt X, GE Marti and TA Fleisher. (1997). Comparative analysis of whole blood lysis methods for flow cytometry. Cytometry 30:124-133.
19. Gutensohn K, S Serke and V Kretschmer. (1996). Flow cytometry analysis of CD34 expressing hematopoietic cells in blood and cytapheresis products. Infusionstherapie Transfusionsmedizin 23:1-23.
20. Cassens U, B Greve, K Tapernon, B Nave, E Severin, W Sibrowski and W Göhde. (2002). A novel true volumetric method for the determination of residual leucocytes in blood components. Vox Sanguinis 82:198-206.
21. + cell counting in phosphatebuffered saline-diluted leukapheresis samples. Cytometry 43:154-160.
22. + hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte lysing reagents. J Immunol Methods 239:13-23.
23. Vuorte J, SE Jansson and H Repo. (2001). Evaluation of red blood cell lysing solutions in the study of neutrophil oxidative burst by the DCFH assay. Cytometry 43:290-296.
24. Gutensohn K, I Carrero, W Krueger, N Kroeger, P Schäfer, K Luedemann and P Kuehnl. (1999). Semi-automated flow cytometric analysis of CD34-expressing hematopoietic cells in peripheral blood progenitor cell apheresis products. Transfusion 39:1220-1226.
25. + cells. Transfusion 39:864-872.
26. Basso G and F Timeus (1998). Cytofluorimetric analysis of CD34 cells. Bone Marrow Transplant 22:17-20.
27. Greve B, U Cassens, C Westerberg, W Göhde jun, W Sibrowski, D Reichelt and W Göhde (2003). A new nolyse, no-wash flow-cytometric method for the determination of CD4 T cells in blood samples. Transfusion Med Hemother 30:8-13.
28. + cells by flow cytometry. Cytometry 50:249-253.
29. + hematopoietic progenitor cells: correlation with colony assay. Int J Biol Markers 17:259-267.