At-line monitoring of bioreactor protein production by surface plasmon resonance

Biotechnology and Bioengineering

Volumn 100, Issue 1, 2008, Pages 184-188

  Jacquemart, R ^a   Chavane, N ^a   Durocher, Y ^b   Hoemann, C ^a   De Crescenzo, G ^a   Jolicoeur, M ^a  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

At line protein monitoring; Bioreactor protein production; HEK 293 cells; Surface plasmon resonance; Transient transfection

Indexed keywords

ANIMAL CELL CULTURE; BIOREACTORS; BIOSENSORS; SURFACE PLASMON RESONANCE;

AT-LINE PROTEIN MONITORING; BIOREACTOR PROTEIN PRODUCTION; HEK 293 CELLS; TRANSIENT TRANSFECTION;

PROTEINS;

AMINO ACID SEQUENCE; ARTICLE; BIOREACTOR; CONTROLLED STUDY; DRUG ACTIVITY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MAMMAL CELL; PROTEIN DETERMINATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; REACTOR MONITORING; SURFACE PLASMON RESONANCE; TRANSIENT TRANSFECTION;

BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE; HUMANS; KIDNEY; MONITORING, PHYSIOLOGIC; ONLINE SYSTEMS; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE;

MAMMALIA;

EID: 42549088112     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21725     Document Type: Article

References (9)

1. De Crescenzo G, Litowski JR, Hodges RS, O'Connor-McCourt MD. 2003a. Real-time monitoring of the interactions of two-stranded de novo designed coiled-coils: Effect of chain length on the kinetic and thermodynamic constants of binding. Biochemistry 42:1754-1763.
2. De Crescenzo G, Pham PL, Durocher Y, O'Connor-McCourt MD. 2003b. Transforming growth factor-beta (TGF-beta) binding to the extracellular domain of the type II TGF-beta receptor: Receptor capture on a biosensor surface using a new coiled-coil capture system demonstrates that avidity contributes significantly to high affinity binding. J Mol Biol 328:1173-1183.
3. De Crescenzo G, Pham PL, Durocher Y, Chao H, O'Connor-McCourt MD. 2004. Enhancement of the antagonistic potency of TGF-beta receptor extracellular domains by coiled-coil induced homo- and hetero-dimerization. J Biol Chem 279:26013-26018.
4. Durocher Y, Perret S, Kamen A. 2002. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucl Acids Res 30:E9.
5. Jolicoeur M, Chavarie C, Carreau PJ, Archambault J. 1992. Development of a helical-ribbon impeller bioreactor for high-density plant cell suspension culture. Biotechnol Bioeng 39:511-521.
6. Jones JJ, Bridges AM, Fosberry AP, Gardner S, Lowers RR, Newby RR, James PJ, Hall RM, Jenkins O. 2004. Potential of real-time measurement of GFP-fusion proteins. J Biotechnol 109:201-211.
7. Jones JJ, Wilkins CL, Cai Y. 2005. Real-time monitoring of recombinant bacterial proteins by mass spectrometry. Biotechnol Prog 21:1754-1758.
8. Pol E, Karlsson R, Roos H, Jansson A, Xu B, Larsson A, Jarhede T, Franklin G, Fuentes A, Persson S. 2007. Biosensor-based characterization of serum antibodies during development of an IgE immunotherapeutic against allergy and asthma. J Mol Recognit 20:22-31.
9. Rich RL, Myszka DG. 2000. Advances in surface plasmon resonance biosensor analysis. Curr Opin Biotechnol 11:54-61.