Transforming growth factor-beta (TGF-β) binding to the extracellular domain of the type II TGF-β receptor: Receptor capture on a biosensor surface using a new coiled-coil capture system demonstrates that avidity contributes significantly to high affinity binding

Journal of Molecular Biology

Volumn 328, Issue 5, 2003, Pages 1173-1183

  De Crescenzo, Gregory ^a   Pham, Phuong L ^a   Durocher, Yves ^a   O'Connor McCourt, Maureen D ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Biosensor; Coiled coil; Kinetics; TGF ; Type II TGF receptor

Indexed keywords

BINDING PROTEIN; DIMER; ISOPROTEIN; LIGAND; PROTEIN SUBUNIT; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA RECEPTOR;

ARTICLE; BINDING AFFINITY; BIOSENSOR; CELL SURFACE; KINETICS; ORIENTATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN IMMOBILIZATION; PROTEIN STRUCTURE; RECEPTOR AFFINITY; RECEPTOR BINDING; STOICHIOMETRY;

EID: 0242684423     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(03)00360-7     Document Type: Article

References (28)

1. Massague J., Blain S.W., Lo R.S. TGFbeta signaling in growth control, cancer, and heritable disorders. Cell. 103:2000;295-309.
2. Massague J., Wotton D. Transcriptional control by the TGF-beta/Smad signaling system. EMBO J. 19:2000;1745-1754.
3. Roberts A.B., McCune B.K., Sporn M.B. TGF-beta: regulation of extracellular matrix. Kidney Int. 41:1992;557-559.
4. Sporn M.B., Roberts A.B. Transforming growth factor-beta: recent progress and new challenges. J. Cell Biol. 119:1992;1017-1021.
5. Wakefield L.M., Colletta A.A., McCune B.K., Sporn M.B. Roles for transforming growth factors-beta in the genesis, prevention, and treatment of breast cancer. Cancer Treat. Res. 61:1992;97-136.
6. Massague J., Chen Y.G. Controlling TGF-beta signaling. Genes Dev. 14:2000;627-644.
7. Wrana J.L., Attisano L., Carcamo J., Zentella A., Doody J., Laiho M., et al. TGF beta signals through a heteromeric protein kinase receptor complex. Cell. 71:1992;1003-1014.
8. Blobe G.C., Schiemann W.P., Pepin M.C., Beauchemin M., Moustakas A., Lodish H.F., O'Connor-McCourt M.D. Functional roles for the cytoplasmic domain of the type III transforming growth factor beta receptor in regulating transforming growth factor beta signaling. J. Biol. Chem. 276:2001;24627-24637.
9. Lopez-Casillas F., Wrana J.L., Massague J. Betaglycan presents ligand to the TGF beta signaling receptor. Cell. 73:1993;1435-1444.
10. Cheifetz S., Hernandez H., Laiho M., ten Dijke P., Iwata K.K., Massague J. Distinct transforming growth factor-beta (TGF-beta) receptor subsets as determinants of cellular responsiveness to three TGF-beta isoforms. J. Biol. Chem. 265:1990;20533-20538.
11. De Crescenzo G., Grothe S., Zwaagstra J., Tsang M., O'Connor-McCourt M.D. Real-time monitoring of the interactions of transforming growth factor- beta (TGF-beta) isoforms with latency-associated protein and the ectodomains of the TGF-beta type II and III receptors reveals different kinetic models and stoichiometries of binding. J. Biol. Chem. 276:2001;29632-29643.
12. Massague J. The transforming growth factor-beta family. Annu. Rev. Cell Biol. 6:1990;597-641.
13. Chao H., Houston M.E. Jr, Grothe S., Kay C.M., O'Connor-McCourt M., Irvin R.T., Hodges R.S. Kinetic study on the formation of a de novo designed heterodimeric coiled-coil: use of surface plasmon resonance to monitor the association and dissociation of polypeptide chains. Biochemistry. 35:1996;12175-12185.
14. De Crescenzo G., Grothe S., Lortie R., Debanne M.T., O'Connor-McCourt M. Real-time kinetic studies on the interaction of transforming growth factor alpha with the epidermal growth factor receptor extracellular domain reveal a conformational change model. Biochemistry. 39:2000;9466-9476.
15. Fisher R.J., Fivash M. Surface plasmon resonance based methods for measuring the kinetics and binding affinities of biomolecular interactions. Curr. Opin. Biotechnol. 5:1994;389-395.
16. Morton T.A., Myszka D.G., Chaiken I.M. Interpreting complex binding kinetics from optical biosensors: a comparison of analysis by linearization, the integrated rate equation, and numerical integration. Anal. Biochem. 227:1995;176-185.
17. Durocher Y., Perret S., Kamen A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucl. Acids Res. 30:2002;E9.
18. Guimond A., Sulea T., Pepin M.C., O'Connor-McCourt M.D. Mapping of putative binding sites on the ectodomain of the type II TGF-beta receptor by scanning-deletion mutagenesis and knowledge-based modeling. FEBS Letters. 456:1999;79-84.
19. Khaleghpour K., Kahvejian A., De Crescenzo G., Roy G., Svitkin Y.V., Imataka H., et al. Dual interactions of the translational repressor Paip2 with poly(A) binding protein. Mol. Cell. Biol. 21:2001;5200-5213.
20. Myszka D.G. Improving biosensor analysis. J. Mol. Recognit. 12:1999;279-284.
21. De Crescenzo G., Litowski J.R., Hodges R.S., O'Connor-McCourt M.D. Real-time monitoring of the interactions of two-stranded de novo designed coiled-coils: effect of chain length on the kinetic and thermodynamic constants of binding. Biochemistry. 42:2003;1754-1763.
22. Matsuzaki K., Kan M., McKeehan W.L. Reconstitution of a pentameric complex of dimeric transforming growth factor beta ligand and a type I, II, III receptor in baculoviral- infected insect cells. In Vitro Cell Dev. Biol. Anim. 32:1996;345-360.
23. Hart P.J., Deep S., Taylor A.B., Shu Z., Hinck C.S., Hinck A.P. Crystal structure of the human TbetaR2 ectodomain-TGF-beta3 complex. Nature Struct. Biol. 9:2002;203-208.
24. Chao H., Bautista D.L., Litowski J., Irvin R.T., Hodges R.S. Use of a heterodimeric coiled-coil system for biosensor application and affinity purification. J. Chromatog. B Biomed. Sci. Appl. 715:1998;307-329.
25. Tripet B., Yu L., Bautista D.L., Wong W.Y., Irvin R.T., Hodges R.S. Engineering a de novo-designed coiled-coil heterodimerization domain off the rapid detection, purification and characterization of recombinantly expressed peptides and proteins. Protein Eng. 9:1996;1029-1042.
26. Myszka D.G., Morton T.A., Doyle M.L., Chaiken I.M. Kinetic analysis of a protein antigen-antibody interaction limited by mass transport on an optical biosensor. Biophys. Chem. 64:1997;127-137.
27. Tripet B., De Crescenzo G., Grothe S., O'Connor-McCourt M., Hodges R.S. Kinetic analysis of the interactions between Troponin C and the C-terminal Troponin I regulatory region and validation of a new peptide delivery/capture system used for surface plasmon resonance. J. Mol. Biol. 2002.
28. Cote J., Garnier A., Massie B., Kamen A. Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells. Biotechnol. Bioeng. 59:1998;567-575.