Variable expression of Cre recombinase transgenes precludes reliable prediction of tissue-specific gene disruption by tail-biopsy genotyping

PLoS ONE

Volumn 2, Issue 10, 2007, Pages

  Schulz, Tim J ^a,b   Glaubitz, Markus ^b   Kuhlow, Doreen ^b   Thierbach, René ^c   Birringer, Marc ^a   Steinberg, Pablo ^c   Pfeiffer, Andreas F H ^b   Ristow, Michael ^a,b  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b GERMAN INSTITUTE OF HUMAN NUTRITION   (Germany)

^c UNIVERSITY OF POTSDAM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; CRE RECOMBINASE; INTEGRASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; GENE DISRUPTION; GENE EXPRESSION REGULATION; GENE INSERTION; GENOTYPE; IN VIVO STUDY; MOUSE; NONHUMAN; POST HOC ANALYSIS; PREDICTION; RELIABILITY; TISSUE SPECIFICITY; TRANSGENE; TRANSGENIC MOUSE; ANIMAL; BIOPSY; DNA SEQUENCE; GENETIC PROCEDURES; GENETIC RECOMBINATION; GENETICS; METABOLISM; PHENOTYPE; REPORTER GENE; REPRODUCIBILITY; TAIL;

MUS MUSCULUS;

ANIMALS; BIOPSY; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION; GENES, REPORTER; GENETIC TECHNIQUES; GENOTYPE; INTEGRASES; MICE; MICE, TRANSGENIC; PHENOTYPE; RECOMBINATION, GENETIC; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA; TAIL; TRANSGENES;

EID: 42449098832     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0001013     Document Type: Article

References (22)

1. Gu H, Zou YR, Rajewsky K (1991) Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell 73: 1155-1164.
2. Nagy A (2000) Cre recombinase: the universal reagent for genome tailoring Genesis 26: 99-109.
3. Spofford JB (1959) Parental control of position-effect variegation: I. Parental heterochromatin and expression of the white locus in compound-X Drosophila melanogaster. Proc Natl Acad Sci U S A 45: 1003-1007.
4. Baker WK (1968) Position-effect variegation. Adv Genet 14: 133-169.
5. Singh PB (1994) Molecular mechanism of cellular determination: their relation to chromatin structure and parental imprinting. J Cell Sci 107 ( Pt 10): 2653-2668.
6. Garrick D, Sutherland H, Robertson G, Whitelaw E (1996) Variegated expression of a globin transgenic correlates with chromatin accessibility but not methylation status. Nucleic Acids Res 24: 4902-4909.
7. Goldman IL, Kadulin SG, Razin SV (2004) Transgenic animals in medicine: integration and expression of foreign genes, theoretical and applied aspects. Med Sci Moni. 10: RA274-285.
8. Dobie KW, Lee M, Fautes JA, Graham E, Clark AJ, et al. (1996) Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus. Proc Natl Acad Sci U S A 93: 6659-6664.
9. Feng YQ, Lorincz MC, Fiering S, Greally JM, Bouhassira EE (2001) Position effects are influenced by the orientation of a transgene with respect to flanking chromatin. Mol Cell Biol 21: 298-309.
10. Robertson G, Garrick D, Wilson M, Martin DI, Whitelaw E. (1996) Age-dependent silencing of globin, transgenes in the mouse. Nucleic Acids Res 24: 1465-1471.
11. Postic Q, Shiota M, Niswender KD, Jetton TL, Chen Y, et al. (1999) Dual roles for glucokinase in glucose homeoistasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. J Biol Chem 274: 305-315.
12. Hayhurst GP, Lee YH, Lambert G, Ward JM, Gonzalez FJ (2001) Hepatocyte nuclear factor 4alpha (nuclear receptor 2A1) is essential for maintenance of hepatic gene expression and lipid homeostasis. Mol Cell Biol 21: 1393-1403.
13. Thierbach R, Schulz TJ, Isken F, Voigt A, Mietzner B, et al. (2005) Targeted disruption of hepatic frataxin expression causes impaired mitochondrial function, decreased life span, and tumor growth in mice. Hum Mol Genet 14: 3857-3864.
14. Michael MD, Kulkarni RN, Postic C, Previs SF, Shulman GI, et al. (2000) Loss of insulin signaling in hepatocytes leads to severe insulin resistance and progressive hepatic dysfunction. Mol Cell 6: 87-97.
15. Postic C, Magnuson MA (2000) DNA excision in liver by an albumin-Cre transgene occurs progressively with age. Genesis 26: 149-150.
16. Ristow M, Mulder H, Pomplun D, Schulz TJ, Muller-Schmehl K, et al. (2003) Frataxin-deficiency in pancreatic islets causes diabetes due to loss of beta-cell mass. J Clin Invest 112: 527-534.
17. Puccio H, Simon D, Cossee M, Criqui-Filipe P, Tiziano F, et al. (2001) Mouse models for Friedreich ataxia exhibit cardiomyopathy, sensory nerve defect and Fe-S enzyme deficiency followed by intramitochondrial iron deposits. Nat Genet 27: 181-186.
18. Siomi MC, Tsukumo H, Ishizuka A, Nagami T, Siomi H (2005) A potential link between transgene silencing and poly(A) tails, Rna 11: 1004-1011.
19. Hamanaka S, Nabekura T, Otsu M, Yoshida H, Nagata M, et al. (2006) Stable transgene expression in mice generated from retrovirally transduced embryonic stem cells. Mol Ther.
20. Flanagan JR, Krieg AM, Max EE, Khan AS (1989) Negative control region at the 5′ end of murine leukemia virus long terminal repeats. Mol Cell Biol 9: 739-746.
21. Swindle CS, Kim HG, Klug CA (2004) Mutation ot CpGs in the murine stem cell virus retroviral vector long terminal repeat represses silencing in embryonic stem cells. J Biol Chem 279: 34-41.
22. Sheichet SA, Bäumer AT, Stamenkovic D, Sauer H, Pfeiffer AF, et al. (2002) Frataxin promotes antioxidant defense in a thiol-dependent manner resulting in diminished malignant transformation in vitro. Hum Mol Genet 11: 815-821.