A new method to extract dental pulp DNA: Application to universal detection of bacteria

PLoS ONE

Volumn 2, Issue 10, 2007, Pages

  Tran Hung, Lam ^a   Tran Thi, Ny ^a   Aboudharam, Gérard ^a   Raoult, Didier ^a   Drancourt, Michel ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA 16S; REAGENT; RESIN; RIBOSOME DNA; RNA POLYMERASE BETA SUBUNIT; BACTERIAL DNA; BACTERIAL PROTEIN; DNA; PRIMER DNA; RNA 16S; RPOB PROTEIN, STREPTOCOCCUS PNEUMONIAE; UNCLASSIFIED DRUG;

ARTICLE; BACTEREMIA; BACTERIAL COLONIZATION; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA EXTRACTION; DNA SEQUENCE; GENE AMPLIFICATION; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PERIODONTIUM; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; TOOTH PULP; BIOCHEMISTRY; CHEMISTRY; DENTISTRY; GENETIC PROCEDURES; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY;

ANIMALIA; BACTERIA (MICROORGANISMS);

BACTEREMIA; BACTERIAL PROTEINS; BIOCHEMISTRY; DENTAL PULP; DENTISTRY; DNA; DNA PRIMERS; DNA, BACTERIAL; GENETIC TECHNIQUES; HUMANS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

EID: 42349093247     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0001062     Document Type: Article

References (41)

1. Delivanis PD, Snowden RB, Doyle RJ (1981) Localization of blood-borne bacteria in instrumented unfilled root canals. Oral Surg Oral Med Oral Pathol Oct 52(4): 430-2.
2. Tziafas D (1989) Experimental bacterial anachoresis in dog dental pulps capped with calcium hydroxide. J Endod Dec 15(12): 591-5.
3. Hoshino E, Ando N, Sato M, Kota K (1992) Bacterial invasion of non-exposed dental pulp. Int Endod J Jan 25(1): 2-5.
4. Aboudharam G, La Scola B, Raoult D, Drancourt M (2000) Detection of Coxiella burnetii DNA in dental pulp during experimental bacteremia. Microb Pathog 28(4): 249-54.
5. Aboudharam G, Drancourt M, Raoult D (2004) Culture of C. burnetii from the dental pulp of experimentally infected guinea pigs. Microb Pathog Jun 36(6): 349-50.
6. Glick M, Trope M, Pliskin ME (1989) Detection of HIV in the dental pulp of a patient with AIDS. J Am Dent Assoc 119: 649-50.
7. Glick M, Trope M, Bagasra O, Pliskin ME (1991) Human immunodeficiency virus infection of fibroblasts of dental pulp in seropositive patients. Oral Surg Oral Med Oral Pathol 71: 733-6.
8. Aboudharam G, Fournier PE, Drancourt M, Raoult D, Foucault C, et al. (2004) Molecular detection of Bartonella quintana DNA in the dental pulp of a homeless patient. Eur J Clin Microbiol Infect Dis Dec 23(12): 920-2.
9. Drancourt M, Raoult D (2005) Palaeomicrobiology: current issues and perspectives. Nat Rev Microbiol Jan 3(1): 23-35.
10. Drancourt M, Aboudharam G, Signoli M, Dutour O, Raoult D (1998) Detection of 400-year-old Yersinia pestis DNA in human dental pulp: an approach to the diagnosis of ancient septicemia. Proc Natl Acad Sci USA 95: 12637-40.
11. Raoult D, Aboudharam G, Crubezy E, Larrouy G, Ludes B, et al. (2000) Molecular identification by "suicide PCR" of Yersinia pestis as the agent of Medieval Black Death. Proc Natl Acad Sci U S A 97(23): 12800-3.
12. Drancourt M, Raoult D (2004) Molecular detection of Yersinia pestis in dental pulp. Microbiology Feb 150(Pt 2): 263-4.
13. Wiechmann I, Grupe G (2005) Detection of Yersinia pestis DNA in two early medieval skeletal finds from Aschheim (Upper Bavaria, 6th century A.D.). Am J Phys Anthropol Jan 126(1): 48-55.
14. Papagrigorakis MJ, Yapijakis C, Synodinos PN, Baziotopoulou-Valavani E (2006) DNA examination of ancient dental pulp incriminates typhoid fever as a probable cause of the Plague of Athens. Int J Infect Dis May 10(3): 206-14.
15. Drancourt M, Tran-Hung L, Courtin J, Lumley H, Raoult D (2005) Bartonella quintana in a 4000-year-old human tooth. J Infect Dis Feb 15 191(4): 607-11.
16. La VD, Clavel B, Lepetz S, Aboudharam G, Raoult D, et al. (2004) Molecular detection of Bartonella henselae DNA in the dental pulp of 800-year-old French cats. Clin Infect Dis Nov 1 39(9): 1391-4.
17. Woodward SR, King MJ, Chiu NM, Kuchar MJ, Griggs CW (1994) Amplification of ancient nuclear DNA from teeth and soft tissues. PCR Methods Appl Feb 3(4): 244-7.
18. Calvo F, Ricaut F, Keyser C, et al. (2001) Etude de l'ADN ancien au niveau de la pulpe dentaire de la série ostéologique de Saint Côme et Damien. Anthropo 1: 21-9.
19. Rudbeck L, Gilbert MT, Willerslev E, Hansen AJ, Lynnerup N, et al. (2005) mtDNA analysis of human remains from an early Danish Christian cemetery. Am J Phys Anthropol Oct 128(2): 424-9.
20. Restagno G, Ferrone M, Doriguzzi C, Palmucci L, Mongini T, et al. (1995) Carrier detection of Duchenne, muscular dystrophy through analysis of DNA from deciduous teeth of a dead affected child. Prenat Diagn Jul 15(7): 672-4.
21. Leonard H, Davis MR, Turbett GR, Laing NG, Bower C, et al. (2005) Effectiveness of posthumous molecular diagnosis from a kept baby tooth. Lancet Oct 29 366(9496): 1584.
22. Gilbert MT, Cuccui J, White W, Lynnerup N, TitbaU RW, et al. (2004) Absence of Yersinia pestis-specific DNA in human teeth from, five European excavations of putative plague victims. Microbiology Feb 150(Pt 2): 341-54.
23. Gilbert MT, Willerslev E, Hansen AJ, Barnes I, Rudbeck L, et al. (2003) Distribution patterns of postmortem damage in human mitochondrial DNA. Am J Hum Genet Jan 72(1): 32-47.
24. Borst A, Box AT, Fluit AC (2004) False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy. Eur J Clin Microbiol Infect Dis Apr 23(4): 289-99.
25. Rand KH, Houck H (1990) Taq polymerase contains bacterial DNA of unknown origin. Mol Cell Probes Dec 4(6): 445-50.
26. Hughes MS, Beck LA, Skucc RA (1994) Identification and elimination of DNA sequences in Taq DNA polymerase. J Clin Microbiol Aug 32(8): 2007-8.
27. Grahn N, Olofsson M, Ellnebo-Svedlund K, Monstein HJ, Jonasson J (2003) Identification of mixed bacterial DNA contamination in broad-range PCR amplification of 16S rDNA V1 and V3 variable regions by pyrosequencing of cloned amplicons. FEMS Microbiol Lett Feb 14 219(1): 87-91.
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring HarborNY: Cold Spring Harbor Laboratory.
29. Wages JMJ, Gai D, Fowler AK (1994) Removal of contaminating-DNA from PCR reagents by ultrafiltration. Biotechniques Jun 16(6): 1014-7.
30. Rothman RE, Majmudar MD, Kelen GD, Madico G, Gaydos CA, et al. (2002) Detection of bacteremia in emergency department patients at risk for infective endocarditis using universal 16S rRNA primers in a decontaminated Polymerase Chain Reaction assay. J Infect Dis Dec 1 186(11): 1677-81.
31. Kiska DL, Gilligan PH (2003) Pseudomonas. In: Murray PR, Barori EJ, Jorgensen JH, Pfaller MA, Yolken RH, eds. Washington DC: American Society for Microbiology. pp 719-28.
32. Hoffmann H, Roggenkamp A (2003) Population genetics of the nomenspecies Enterobacter cloacae. Appl.Environ Microbiol Sep 69(9): 5306-18.
33. Fernandez-Baca V, Ballesteros F, Hervas JA, Villalon P, Dominguez MA, et al. (2001) Molecular epidemiological typing of Enterobacter cloacae isolates from a neonatal intensive care unit: three-year prospective study. J Hosp Infect Nov 49(3): 173-82.
34. Kim BN, Ryu J, Kim YS, Woo JH (2002) Retrospective analysis of clinical and microbiological aspects of Klebsiella oxytoca bactoremia over a 10-year period. Eur J Clin Microbiol Infect Dis Jun 21(6): 419-26.
35. Seifert H, Strate A, Schulze A, Pulverer G (1993) Vascular catheter-related bloodstream infection due to Acinetobacter johnoonii (formerly Acinetobacter calcoaceticus var. lwoffi): report of 13 cases. Clin Infect Dis Oct 17(4): 632-6.
36. Doyuk E, Ormerod OJ, Bowler IC (2002) Native valve endocarditis due to Streptococcus vestibularis and Streptococcus oralis. J Infect Jul 45(1): 39-41.
37. Rocas IN, Jung TY, Lee CY, Siqueira JF Jr (2004) Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population. J Endod Jul 30(7): 504-8.
38. Rupf S, Kannengiesser S, Merte K, Pfister W, Sigusch B, et al. (2000) Comparison of profiles of key periodontal pathogens in periodontium and endodontium. Endod Dent Traumatol Dec 16(6): 269-75.
39. Mollet C, Drancourt M, Raoult D (1997) rpoB sequence analysis as a novel basis for bacterial identification. Mol Microbiol 26: 1005-11.
40. Berger P, Adekambi T, Mallet MN, Drancourt M (2005) Prevotella massiliensis sp. nov. isolated from human blood. Res Microbiol Dec 156(10): 967-73.
41. Kim KS, Ko KS, Chang MW, Hahn TW, Hong SK, et al. (2003) Use of rpoB sequences for phylogenetic study of Mycoplasma species. FEMS Microbiol Lett Sep 26 226(2): 299-1305.