Motorcycle exhaust induces reproductive toxicity and testicular interleukin-6 in male rats

Toxicological Sciences

Volumn 103, Issue 1, 2008, Pages 137-148

  Huang, Jing Ying ^a   Liao, Jiunn Wang ^b   Liu, Yi Chun ^a   Lu, Shui Yuan ^c   Chou, Chen Ping ^a,c   Chan, Wei Hung ^a   Chen, Shee Uan ^a   Ueng, Tzuu Huei ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^c TAIWAN AGRICULTURAL CHEMICALS AND TOXIC SUBSTANCES RESEARCH INSTITUTE   (Taiwan)

Author keywords

Air pollution; IL 6; Motorcycle exhaust; Oxidative stress; Sperm; Testis; Testosterone

Indexed keywords

7 ETHOXYCOUMARIN DEETHYLASE; ALPHA TOCOPHEROL; INTERLEUKIN 6; MESSENGER RNA; SUPEROXIDE DISMUTASE; TESTOSTERONE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; CELL DEATH; CONTROLLED STUDY; ENVIRONMENTAL EXPOSURE; ENZYME ACTIVITY; EPIDIDYMIS TAIL; EXHAUST GAS; FEMALE; HISTOPATHOLOGY; INFERTILITY; MALE; MATING SUCCESS; NIDATION; NONHUMAN; OXIDATIVE STRESS; PROTEIN INDUCTION; RAT; REPRODUCTIVE TOXICITY; SEMINIFEROUS TUBULE; SPERMATOCYTE; SPERMATOZOON COUNT; STEROIDOGENESIS; TESTIS ATROPHY; TESTIS WEIGHT; TESTOSTERONE BLOOD LEVEL;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME-LINKED IMMUNOSORBENT ASSAY; INTERLEUKIN-6; LIPID PEROXIDATION; MALE; MOTORCYCLES; OXIDATIVE STRESS; RATS; RATS, WISTAR; REPRODUCTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TESTIS; TESTOSTERONE; VEHICLE EMISSIONS;

RATTUS; RATTUS NORVEGICUS;

EID: 42149191907     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfn020     Document Type: Article

References (45)

1. Agardh, C. D., Israelsson, B., Thuesen-Olesen, B., and Agardh, E. (2002). Application of quantitative competitive polymerase chain reaction for measurements of mRNA from antioxidative enzymes in the diabetic rat retina and kidney. Metabolism 51, 1279-1284.
2. Aggarwal, B. B., Shishodia, S., Sandur, S. K., Pandey, M. K., and Sethi, G. (2006). Inflammation and cancer: How hot is the link? Biochem. Pharmacol. 72, 1605-1621.
3. Azzi, A., Gysin, R., Kempna, P., Munteanu, A., Negis, Y., Villacorta, L., Visarius, T. and Zingg, J. M. (2004). Vitamin E mediates cell signaling and regulation of gene expression. Ann. N. Y. Acad. Sci. 1031, 86-95.
4. Baltes, M. R. H., Dubois, J. G., and Hanocq, M. (1998). Ethyl acetate extraction procedure and isocratic high-performance liquid chromatographic assay for testosterone metabolites in cell microsomes. J. Chromatogr. B Biomed. Sci. Appl. 706, 201-207.
5. Boockfor, F. R., Wang, D., Lin, T., Nagpal, M. L., and Spangelo, B. L. (1994). Interleukin-6 secretion from rat Leydig cells in culture. Endocrinology 134, 2150-2155.
6. Bornstein, S. R., Rutowski, H., and Vrezas, I. (2004). Cytokines and steroidogenesis. Mol. Cell. Endocrinol. 215, 135-141.
7. Chomczynski, P., and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
8. Flohö, L., and Günzler, W. A. (1984). Assays of glutathione peroxidase. Methods Enzymol. 105, 114-121.
9. 3 particles Toxicol. Lett. 121, 107-117.
10. Gray, L. E., Jr. (1998) Xenoendocrine disruptors: Laboratory studies on male reproductive effects. Toxicol. Lett. 102-103, 331-335.
11. Greenlee, W. F., and Poland, A. (1978). An improved assay of 7-ethoxycoumarin O-deethylase activity: Induction of hepatic enzymic activity in C57BL/6J and DBA/2J mice by phenobarbital, 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin. J. Phamacol. Exp. Ther. 205, 596-605.
12. Habig, W. H, Pabst, M. J., and Jakoby, W. B. (1974), Glutathione S-transferase. The-first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139.
13. Han, Y., Runge, M. S., and Brasler, A. R. (1999). Angiotensin II induces interleukin-6 transcription in vascular smooth muscle cells through pleiotropic activation of nuclear factor-κB transcription factors. Circ. Res. 84, 695-703.
14. Hedger, M. P., and Meinhardt, A. (2003). Cytokines and the immune-testicular axis. J. Reprod. Immunol. 58, 1-26.
15. Hess, R. A. (1990). Quantitative and qualitative characteristics of stages and transitions in the cycle of the rat seminiferous epithelium: Light microscopic examinations of perfusion-fixed and plastic-embedded testes. Biol. Reprod. 43, 525-542.
16. Inyang, F., Ramesh, A., Kopsombut, P., Niaz, M. S., Hood, D. B., Nyanda, A. M., and Archibong, A. E. (2003). Disruption of testicular steroidogenesis and epididymal function by inhaled benzo(a)pyrene. Reprod. Toxicol. 17, 527-537.
17. 16 hydrocarbons using dual-column capillary GC: Application to exhaust emissions from passenger car and motorcycle engine. J. Chromatogr. Sci. 33, 34-48.
18. Jollow, D. J., Mitchell, J. R., Zampaglione, N., and Gillette, J. R. (1974). Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11, 151-169.
19. Kem, S., Robertson, S. A., Mau, V. J., and Maddocks, S. (1995). Cytokine secretion by macrophages in the rat testis. Biol. Reprod. 53 1407-1416.
20. Koo, M.-L., Jee, S.-H., Chou, M.-H., and Ueng, T.-H. (1998). Involvement of oxidative stress in motorcycle exhaust particle-induced DNA damage and inhibition of intercellular communication. Mutat. Res. 413 143-150.
21. Latchournycandane, C., and Mathur, P. P. (2002). Effect of vitamin E on reactive oxygen species-mediated 2,3.7,8-tetrachlorodibenzo-p-dioxin toxicity in rat testis. J. Appl. Toxicol. 22, 345-351.
22. Lee, C.-C., Liao, J.-W., and Kang, J.-J. (2004). Motorcycle exhaust particles induce airway inflammation and airway hyperresponsiveness in BALB/C mice. Toxicol. Sci. 79, 326-334.
23. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein determinations with Folin phenol reagent. J. Biol. Chem. 193, 265-275.
24. Misra, H. P., and Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 247, 3170-3175.
25. Moore, J. A., Daston, G. P., Faustman, E., Golub, M. S., Hart, W. L., Hughes, C., Jr, Kimmel, C. A., Lamb, J. C., IV, Schwetz, B. A., and Scialli, A. R. (1995). An evaluative process for assessing human reproductive and developmental toxicity of agents. Reprod. Toxicol. 9, 61-95.
26. Naz, R. K., and Kaplan, P. (1994). Increased levels of interleukin-6 in seminal plasma of infertile men. J. Androl. 15, 220-227.
27. Oakberg, E. F. (1956). A description of spermatogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Am. J. Anat. 99, 391-413.
28. Ogata, T., Miyauchi, T., Sakai, S., Takanashi, M., Irukayama-Tomobe, Y., and Yamaguchi, I. (2004). Myocardial fibrosis and diastolic dysfunction in deoxycorticosterone acetate-salt hypertensive rats is ameliorated by the peroxisome proliferator-activated receptor-alpha activator fenofibrate, partly by suppressing inflammatory responses associated with the nuclear factor-kappa-B pathway. J. Am. Coll. Cardiol. 43, 1481-1488.
29. Ohkawa, H., Ohishi, N., and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358.
30. Peinnequin, A., Mouret, C., Birot, O., Alonso, A., Mathieu, J., Clarençon, D., Agay, D., Chancerelle, Y., and Multon, E. (2004). Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green. BMC Immunol. 5, 3-12.
31. Pippenger, C. E., Browne, R. W., and Armstrong, D. (1998). Regulatory antioxidant enzymes. Methods Mol. Biol. 108, 299-313.
32. Ryan, D. E., and Levin, W. (1999). Purification and characterization of hepatic microsomal cytochrome P-450. Pharmacol. Ther. 45, 153-239.
33. Safe, S. (2001). Molecular biology of the Ah receptor and its role in carcinogenesis. Toxicol. Lett. 120, 1-7.
34. Somers, C. M., McCarty, B. E., Malek, F., and Quinn, J. S. (2004). Reduction of particulate air pollution lowers the Ask of heritable mutations in mice. Science 304, 1008-1010.
35. Syed, V., Gérard, N., Kaipia, A., Bardin, C. W., Parvinen, M., and Jégou, B. (1993). Identification, ontogeny, and regulation of an interleukin-6-like factor in the rat seminiferous tubule. Endocrinology 132, 293-299.
36. Tzeng, H.-P., Yang, R.-S., Ueng, T.-H., Lin-Shiau, S. Y., and Liu, S.-H. (2003). Motorcycle exhaust particulates enhance vasoconstriction in organ culture of rat aortas involve reactive oxygen species. Toxicol. Sci. 75, 66-73.
37. Ueng, T.-H., Hwapg, W.-P., Chen, R.-M., Wang, H.-W., Kuo, M.-L., Park, S. S., and Guengerich, F. P. (1998). Effect of motorcycle exhaust on cytochrorne P450-dependent monooxygenases and glutathione S-transferase in rat tissues. J. Toxicol. Environ. Health A 54, 509-527.
38. Ueng, T.-H., Hu, S.-H., Chen, R.-M., Wang, H.-W., and Kuo, M.-L. (2000). Induction of cytochrome P450 1A1 in human hepatoma HepG2 and lung carcinoma NCI-H322 cells by motorcycle exhaust particulate. J. Toxicol. Environ. Health A 60, 101-119.
39. Ueng, T.-H., Hung, C.-C., Kuo, M.-L, Chan, P.-K., Hu, S.-H., Yang, P.-C., and Chang, L. W. (2005). Induction of fibroblast growth factor-9 and inteleukin-1α gene expression by motorcycle exhaust particulate extracts and benzo(a)pyrene in human lung adenocarcinoma cells. Toxicol. Sci. 87, 483-496.
40. Ueng, T.-H., Wang, H.-W., Huang, Y.-P., and Hang, C.-C. (2004a). Antiestrogenic effects of motorcycle exhaust particulate in MCF-7 human breast cancer cells and immature female rats. Arch. Environ. Contam. Toxicol. 46, 454-462.
41. Ueng, T.-H., Wang, H.-W., Hung, C.-C., and Chang, H.-L. (2004b). Effects of motorcycle exhaust inhalation exposure on cytochrome P-459 2B1, antioxidant enzymes, and lipid peroxidation in rat liver and lung. J. Toxicol. Environ. Health A 67, 875-888.
42. Vine, M. F., Tse, C. K., Hu, P., and Thruong, K. Y. (1996). Cigarette smoking and semen quality. Fertil. Steril. 65, 835-842.
43. Wang, H.-W., Chen, T.-L., Yang, P.-C., and Ueng, T.-H. (2001). Induction of cytochromes P450 1A1 and 1B1 by emodin in human lung adenocarcinoma cell line CL5. Drug Metab. Dispos. 29, 1220-1235.
44. Watanabe, N., and Oonuki, Y. (1999). Inhalation of diesel exhaust affects spermatogenesis in growing male rats, Environ. Health Perspect. 107, 539-544.
45. Yoshida, S., Sagai, M., Oshio, S., Umeda, T., Ihara, T., Sugamata, M., Sugawara, I., and Takeda, K. (1999). Exposure to diesel exhaust effects the male reproductive, system of mice. Int. J. Androl. 22, 307-315.