Effects of zinc bacitracin, bird age and access to range on bacterial microbiota in the ileum and caeca of broiler chickens

Journal of Applied Microbiology

Volumn 104, Issue 5, 2008, Pages 1372-1382

  Gong, J ^a,b   Yu, H ^a   Liu, T ^a   Gill, J J ^a,c   Chambers, J R ^a   Wheatcroft, R ^a   Sabour, P M ^a  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^b GUELPH RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^c TEXAS A AND M UNIVERSITY   (United States)

Author keywords

16S rRNA; Access to range; Bacitracin; Bird age; Chicken; Intestinal microbiota

Indexed keywords

ANTIBIOTICS; BACTERIA; BIRDS; DNA SEQUENCES; ELECTROPHORESIS; GENE ENCODING; RNA; ZINC;

16S RRNA; ACCESS TO RANGE; BACITRACIN; BIRD AGE; CHICKEN; DENATURING GRADIENT GEL ELECTROPHORESIS; DIETARY TREATMENTS; EFFECT OF ZINCS; INTESTINAL MICROBIOTA; MICROBIOTAS;

POLYMERASE CHAIN REACTION;

BACITRACIN ZINC; RNA 16S;

AGE-RELATED DIFFERENCE; ANTIBIOTICS; BACTERIUM; COMMUNITY COMPOSITION; DIET; DIGESTIVE SYSTEM; DISEASE TREATMENT; EFFICIENCY MEASUREMENT; POLYMERASE CHAIN REACTION; POULTRY; SPECIES RICHNESS; ZINC;

ACCESS TO RANGE; AGE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIFIDOBACTERIUM; BIRD; CECUM; CHICKEN; DENATURING GRADIENT GEL ELECTROPHORESIS; DIET; DNA SEQUENCE; DRUG EFFECT; ILEUM; INTESTINE FLORA; LACTOBACILLUS; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PARAMETER; POLYMERASE CHAIN REACTION; POULTRY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

AVES; BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; GALLUS GALLUS;

EID: 42149108601     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03699.x     Document Type: Article

References (42)

1. Amit-Romach, E., Sklan, D. Unil, Z. (2004) Microflora ecology of the chicken intestine using 16S ribosomal DNA primers. Poult Sci 83, 1093 1098.
2. Apajalahti, J.H., Sarkilahti, L.K., Maki, B.R., Heikkinen, J.P., Nurminen, P.H. Holben, W.E. (1998) Effective recovery of bacterial DNA and percent-guanine-plus-cytosine-based analysis of community structure in the gastrointestinal tract of broiler chickens. Appl Environ Microbiol 64, 4084 4088.
3. Apajalahti, J.H., Kettunen, A., Bedford, M.R. Holben, W.E. (2001) Percent G + C profiling accurately reveals diet-related differences in the gastrointestinal microbial community of broiler chickens. Appl Environ Microbiol 67, 5656 5667.
4. Barrow, P. (1992) Probiotics for chickens. In Probiotics, The Scientific Basis ed. Fuller, R. pp. 225 257 London, Glasgow, New York, Tokyo, Melbourne, Madras : Chapman and Hall.
5. Collier, C.T., van der Klis, J.D., Deplancke, B., Anderson, D.B. Gaskins, H.R. (2003a) Effects of tylosin on bacterial mucolysis, Clostridium perfringens colonization, and intestinal barrier function in a chick model of necrotic enteritis. Antimicrob Agents Chemother 47, 3311 3317.
6. Collier, C.T., Smiricky-Tjardes, M.R., Albin, D.M., Wubben, J.E., Gabert, V.M., Deplancke, B., Bane, D., Anderson, D.B. et al. (2003b) Molecular ecological analysis of porcine ileal microbiota responses to antimicrobial growth promoters. J Anim Sci 81, 3035 3045.
7. Corrier, D.E., Nisbet, D.J., Scanlan, C.M., Hollister, A.G. DeLoach, J.R. (1995) Control of Salmonella typhimurium colonization in broiler chicks with a continuous flow characterized culture of cecal bacteria. Poult Sci 74, 916 924.
8. Dumonceaux, T.J., Hill, J.E., Hemmingsen, S.M. van Kessel, A.G. (2006) Characterization of intestinal microbiota and response to dietary virginiamycin supplementation in the broiler chicken. Appl Environ Microbiol 72, 2815 2823.
9. Engberg, R.M., Hedemann, M.S., Leser, T.D. Jensen, B.B. (2000) Effect of zinc bacitracin and salinomycin on intestinal microflora and performance of broilers. Poult Sci 79, 1311 1319.
10. European Commission. May 2001, posting date. 2nd opinion on anti-microbial resistance [Online]. Brussels, Belgium : European Commission. Available at: http://europa.eu.int/comm/food/fs/sc/ssc/out203_en.pdf.
11. Fairchild, A.S., Smith, J.L., Idris, U., Lu, J., Sanchez, S., Purvis, L.B., Hofacre, C. Lee, M.D. (2005) Effects of orally administered tetracycline on the intestinal community structure of chickens and on tet determinant carriage by commensal bacteria and Campylobacter jejuni. Appl Environ Microbiol 71, 5865 5872.
12. Gong, J., Forster, R.J., Yu, H., Chambers, J.R., Sabour, P.M., Wheatcroft, R. Chen, S. (2002a) Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen. FEMS Microbiol Lett 208, 1 7.
13. Gong, J., Forster, R.J., Yu, H., Chambers, J.R., Sabour, P.M., Wheatcroft, R. Chen, S. (2002b) Molecular analysis of bacterial populations in the ileum of broiler chickens and comparison with bacteria in the cecum. FEMS Microbiol Ecol 41, 171 179.
14. Gong, J., Si, W., Forster, R.J., Huang, R., Yu, H., Yin, Y., Yang, C. Han, Y. (2007) 16S rDNA-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca. FEMS Microbiol Ecol 59, 147 157.
15. Guan, L.L., Hagen, K.E., Tannock, G.W., Korver, D.R., Fasenko, G.M. Allison, G.E. (2003) Detection and identification of Lactobacillus species in crops of broilers of different ages by using PCR-denaturing gradient gel electrophoresis and amplified ribosomal DNA restriction analysis. Appl Environ Microbiol 69, 6750 6757.
16. Hentges, D.J. (1992) Gut flora and disease resistance. In Probiotics, The Scientific Basis ed. Fuller, R. pp. 87 110 London, Glasgow, New York, Tokyo, Melbourne, Madras : Chapman and Hall.
17. Hume, M.E., Kubena, L.F., Edrington, T.S., Donskey, C.J., Moore, R.W., Ricke, S.C. Nisbet, D.J. (2003) Poultry digestive microflora biodiversity as indicated by denaturing gradient gel electrophoresis. Poult Sci 82, 1100 1107.
18. Ishibashi, N., Yaeshima, T. Hayasawa, H. (1997) Bifidobacteria: their significance in human intestinal health. Malays J Nutr 3, 149 159.
19. Knarreborg, A., Simon, M.A., Engberg, R.M., Jensen, B.B. Tannock, G.W. (2002) Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages. Appl Environ Microbiol 68, 5918 5924.
20. Lan, P.T.N., Hayashi, H., Sakamoto, M. Benno, Y. (2002) Phylogenetic analysis of cecal microbiota in chicken by the use of 16S rDNA clone libraries. Microbiol Immunol 46, 371 382.
21. Leeson, S. Summers, J.D. (1997) Commercial Poultry Nutrition. Guelph : University Books.
22. Livak, K.J. Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402 408.
23. Lu, J., Idris, U., Harmon, B., Hofacre, C., Maurer, J.J. Lee, M.D. (2003) Diversity and succession of the intestinal bacterial community of the maturing broiler chicken. Appl Environ Microbiol 69, 6816 6824.
24. Mitsuoka, T. (1990) Bifidobacteria and their role in human health. J Ind Microbiol 6, 263 268.
25. Moran, E.T. Jr. (1982) Comparative Nutrition of Fowl and Swine - The Gastrointestinal Systems. Guelph : University Books.
26. Murray, A.E., Hollibaugh, J.T. Orrego, C. (1996) Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments. Appl Environ Microbiol 62, 2676 2680.
27. Muyzer, G., de Waal, E.C. Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59, 695 700.
28. Nielsen, A.T., Liu, W.T., Filipe, C., Grady, L. Jr., Molin, S. Stahl, D.A. (1999) Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor. Appl Environ Microbiol 65, 1251 1258.
29. van Orsouw, N.J., Li, D. Vijg, J. (1997) Denaturing gradient gel electrophoresis (DGGE) increases resolution and informativity of Alu-directed inter-repeat PCR. Mol Cell Probes 11, 95 101.
30. Pedroso, A.A., Menten, J.F. Lambais, M.R. (2005) The structure of bacterial community in the intestines of newly hatched chicks. J Appl Poult Res 14, 232 237.
31. Pedroso, A.A., Menten, J.F., Lambais, M.R., Racanicci, A.M., Longo, F.A. Sorbara, J.O. (2006) Intestinal bacterial community and growth performance of chickens fed diets containing antibiotics. Poult Sci 85, 747 752.
32. Stahl, D.A., Flesher, B., Mansfield, H.R. Montgomery, L. (1988) Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Appl Environ Microbiol 54, 1079 1084.
33. Stavric, S. D'Aoust, J.-Y. (1993) Undefined and defined bacterial preparations for the competitive exclusion of Salmonella in poultry - a review. J Food Prot 56, 173 180.
34. Walter, J., Tannock, G.W., Tilsala-Timisjarvi, A., Rodtong, S., Loach, D.M., Munro, K. Alatossava, T. (2000) Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol 66, 297 303.
35. Whitford, M.F., Forster, R.J., Beard, C.E., Gong, J. Teather, R.M. (1998) Phylogenetic analysis of rumen bacteria by comparative sequence analysis of cloned 16S rRNA genes. Anaerobe 4, 153 163.
36. WHO (2002) Antimicrobial Resistance. Available at: http://www.who.int/ mediacentre/factsheets/ fs194/en/print.html. Accessed 1 October 2006.
37. van der Wielen, P.W., Keuzenkamp, D.A., Lipman, L.J., van Knapen, F. Biesterveld, S. (2002) Spatial and temporal variation of the intestinal bacterial community in commercially raised broiler chickens during growth. Microb Ecol 44, 286 293.
38. Wise, M.G. Siragusa, G.R. (2007) Quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets. J Appl Microbiol 102, 1138 1149.
39. Yin, X.H., Chambers, J.R., Barlow, K., Park, A.S. Wheatcroft, R. (2005) The gene encoding xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp) is conserved among Bifidobacterium species within a more variable region of the genome and both are useful for strain identification. FEMS Microbiol Lett 246, 251 257.
40. Yu, H., Gong, J., Wheatcroft, R., Yin, X., Hill, A., Barlow, K. Sabour, P.M. (2006) Development of a quantitative real-time PCR assay for Bifidobacteria and its application to quantifying bacterial populations in the chicken gut. In Proceedings of Eleventh International Symposium on Microbial Ecology: the Hidden Powers - Microbial Communities in Action. Vienna, Austria, 20 25 August. Abstract# 2610.
41. Zhu, X.Y., Zhong, T., Pandya, Y. Joerger, R.D. (2002) 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Appl Environ Microbiol 68, 124 137.
42. Zhou, H., Gong, J., Brisbin, J., Yu, H., Sanei, B., Sabour, P. Sharif, S. (2008) Appropriate chicken sample size for identifying the composition of broiler intestinal microbiota affected by dietary antibiotics using the PCR-DGGE technique. Poult Sci (in press).