Influence of dTMP on the phenotypic appearance and intracellular persistence of Staphylococcus aureus

Infection and Immunity

Volumn 76, Issue 4, 2008, Pages 1333-1339

  Zander, Johannes ^a   Besier, Silke ^a   Saum, Stephan H ^b   Dehghani, Faramarz ^a   Loitsch, Stefan ^a   Brade, Volker ^a   Wichelhaus, Thomas A ^a  

^a UNIVERSITY HOSPITAL FRANKFURT   (Germany)

^b GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COTRIMOXAZOLE; DEOXYURIDINE PHOSPHATE; FLUOROURACIL; NUCLEOSIDE TRANSPORTER; THYMIDINE; THYMIDINE PHOSPHATE; THYMIDYLATE SYNTHASE; URIDINE; ANTIINFECTIVE AGENT; DIHYDROPTEROATE SYNTHASE; FOLIC ACID ANTAGONIST;

ARTICLE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BACTERIUM COLONY; CELL CULTURE; COLONY FORMING UNIT; CONTROLLED STUDY; CYSTIC FIBROSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; PHENOTYPE; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; CELL LINE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; LUNG; METABOLISM; MICROBIOLOGY; PHYSIOLOGY;

ANTI-BACTERIAL AGENTS; CELL LINE; CYSTIC FIBROSIS; DIHYDROPTEROATE SYNTHASE; FLUOROURACIL; FOLIC ACID ANTAGONISTS; HUMANS; LUNG; PHENOTYPE; STAPHYLOCOCCUS AUREUS; THYMIDINE; TRIMETHOPRIM-SULFAMETHOXAZOLE COMBINATION; URIDINE;

EID: 42149092790     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.01075-07     Document Type: Article

References (33)

1. Abele-Horn, M., B. Schupfner, P. Emmerling, H. Waldner, and H. Goring. 2000. Persistent wound infection after herniotomy associated with small-colony variants of Staphylococcus aureus. Infection 28:53-54.
2. Balwit, J. M., P. van Langevelde, J. M. Vann, and R. A. Proctor. 1994. Gentamicin-resistant menadione and hemin auxotrophic Staphylococcus aureus persist within cultured endothelial cells. J. Infect. Dis. 170:1033-1037.
3. Besier, S., A. Ludwig, K. Ohlsen, V. Brade, and T. A. Wichelhaus. 2007. Molecular analysis of the thymidine-auxotrophic small colony variant phenotype of Staphylococcus aureus. Int. J. Med. Microbiol. 297:217-225.
4. Besier, S., C. Smaczny, C. von Mallinckrodt, A. Krahl, H. Ackermann, V. Brade, and T. A. Wichelhaus. 2007. Prevalence and clinical significance of Staphylococcus aureus small-colony variants in cystic fibrosis lung disease. J. Clin. Microbiol. 45:168-172.
5. Bianchi, V., and J. Spychala. 2003. Mammalian 5′- nucleotidases. J. Biol. Chem. 278:46195-46198.
6. Craig, J. E., Y. Zhang, and M. P. Gallagher. 1994. Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent insertion element, IS 186. Mol. Microbiol. 11:1159-1168.
7. Gibson, R. L., J. L. Burns, and B. W. Ramsey. 2003. Pathophysiology and management of pulmonary infections in cystic fibrosis. Am. J. Respir. Crit. Care. Med. 168:918-951.
8. Gilligan, P. H., P. A. Gage, D. F. Welch, M. J. Muszynski, and K. R. Wait. 1987. Prevalence of thymidine-dependent Staphylococcus aureus in patients with cystic fibrosis. J. Clin. Microbiol. 25:1258-1261.
9. Hallek, M., L. Wanders, S. Strohmeyer, and B. Emmerich. 1992. Thymidine kinase: a tumor marker with prognostic value for non-Hodgkin's lymphoma and a broad range of potential clinical applications. Ann. Hematol. 65:1-5.
10. B modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4. J. Bacteriol. 184:5457-5467.
11. Jarry, T. M., and A. L. Cheung. 2006. Staphylococcus aureus escapes more efficiently from the phagosome of a cystic fibrosis bronchial epithelial cell line than from its normal counterpart. Infect. Immun. 74:2568-2577.
12. Kahl, B. C., G. Belling, P. Becker, C. Indranil, K. Wardecki, K. Hilgert, A. L. Cheung, G. Peters, and M. Herrmann. 2005. Thymidine-dependent Staphylococcus aureus small colony variants are associated with extensive alterations in regulator and virulence gene expression profiles. Infect. Immun. 73:4119-4126.
13. Kahl, B. C., M. Herrmann, A. Schulze Everding, H. G. Koch, K. Becker, E. Harms, R. A. Proctor, and G. Peters. 1998. Persistent infection with small colony variant strains of Staphylococcus aureus in patients with cystic fibrosis. J. Infect. Dis. 177:1023-1029.
14. Kipp, F., W. Ziebuhr, K. Becker, V. Krimmer, N. Höß, G. Peters, and C. von Eiff. 2003. Detection of Staphylococcus aureus by 16S rRNA directed in situ hybridisation in a patient with a brain abscess caused by small colony variants. J. Neurol. Neurosurg. Psychiatry 74:1000-1002.
15. Lowy, F. D. 1998. Staphylococcus aureus infections. N. Engl. J. Med. 339: 520-532.
16. Lyczak, J. B., C. L. Cannon, and G. B. Pier. 2002. Lung infections associated with cystic fibrosis. Clin. Microbiol. Rev. 15:194-222.
17. Müller, R. H., N. Loffhagen, and W. Babel. 1996. Rapid extraction of (di)nucleotides from bacterial cells and determination by ion-pair reversed-phase HPLC. J. Microbiol. Methods 25:29-35.
18. Patching, S. G., S. A. Baldwin, A. D. Baldwin, J. D. Young, M. P. Gallagher, P. J. Henderson, and R. B. Herbert. 2005. The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands. Org. Biomol. Chem. 3:462-470.
19. Peters, G. J., H. H. J. Backus, S. Freemantle, B. van Triest, G. Codacci-Pisanelli, C. L. van der Wilt, K. Smid, J. Lunec, A. H. Calvert, S. Marsh, H. L. McLeod, E. Bloemena, S. Meijer, G. Jansen, C. J. van Groeningen, and H. M. Pinedo. 2002. Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Biochim. Biophys. Acta 1587:194-205.
20. Proctor, R. A., C. von Eiff, B. C. Kahl, K. Becker, P. McNamara, M. Herrmann, and G. Peters. 2006. Small colony variants: a pathogenic form of bacteria that facilitates persistent and recurrent infections. Nat. Rev. Microbiol. 4:295-305.
21. Proctor, R. A., P. van Langevelde, M. Kristjansson, J. N. Maslow, and R. D. Arbeit. 1995. Persisting and relapsing infections associated with small-colony variants of Staphylococcus aureus. Clin. Infect. Dis. 20:95-102.
22. Proctor, R. A. 2006. Respiration and small-colony variants of Staphylococcus aureus, p. 434-442. In V. A. Fischetti, R. P. Novick, J. J. Ferretti, D. A. Portnoy, and J. I. Rood (ed.), Gram-positive pathogens, 2nd ed. ASM Press, Washington, DC.
23. Sadowska, B., A. Bonar, C. von Eiff, R. A. Proctor, M. Chmiela, W. Rudnicka, and B. Rozalska. 2002. Characteristics of Staphylococcus aureus, isolated from airways of cystic fibrosis patients, and their small colony variants. FEMS Immunol. Med. Microbiol. 32:191-197.
24. Saxild, H. H., L. N. Andersen, and K. Hammer. 1996. dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein. J. Bacteriol. 178:424-434.
25. Seifert, H., H. Wisplingholf, P. Schnabel, and C. von Eiff. 2003. Small colony variants of Staphylococcus aureus and pacemaker-related infection. Emerg. Infect. Dis. 9:1316-1318.
26. Stryer, L. 1990. Biosynthese der Nucleotide, p. 627-653. In L. Stryer (ed.), Biochemie. Spektrum der Wissenschaft, Heidelberg, Germany.
27. Tasca, T., C. D. Bonan, G. A. Carli, A. M. Battastini, and J. J. Sarkis. 2003. Characterisation of an ecto-5′-nucleotidase (EC 3.1.3.5) activity in intact cells of Trichomonas vaginalis. Exp. Parasitol. 105:167-173.
28. Vaudaux, P., P. Francois, C. Bisognano, W. L. Kelley, D. P. Lew, J. Schrenzel, R. A. Proctor, P. J. McNamara, G. Peters, and C. von Eiff. 2002. Increased expression of clumping factor and fibronectin-binding proteins by hemB mutants of Staphylococcus aureus expressing small colony variant phenotypes. Infect. Immun. 70:5428-5437.
29. von Eiff, C., C. Heilmann, R. A. Proctor, C. Woltz, G. Peters, and F. Gotz. 1997. A site-directed Staphylococcus aureus hemB mutant is a small-colony variant which persists intracellularly. J. Bacteriol. 179:4706-4712.
30. von Eiff, C., D. Bettin, R. A. Proctor, B. Rolauffs, N. Lindner, W. Winkelmann, and G. Peters. 1997. Recovery of small colony variants of Staphylococcus aureus following gentamicin bead placement for osteomyelitis. Clin. Infect. Dis. 25:1250-1251.
31. von Eiff, C., K. Becker, D. Metze, G. Lubritz, J. Hockmann, T. Schwarz, and G. Peters. 2001. Intracellular persistence of Staphylococcus aureus small-colony variants within keratinocytes: a cause for antibiotic treatment failure in a patient with Darier's disease. Clin. Infect. Dis. 32:1643-1647.
32. von Eiff, C., P. Vaudaux, B. C. Kahl, D. Lew, S. Emler, A. Schmidt, G. Peters, and R. A. Proctor. 1999. Bloodstream infections caused by small-colony variants of coagulase-negative staphylococci following pacemaker implantation. Clin. Infect. Dis. 29:932-934.
33. Zeuthen, E., and L. Rasmussen. 1976. Uridine as an inhibitor of thymidine utilisation and DNA synthesis in L cells with impeded function of thymidylate synthetase. Exp. Cell Res. 98:57-62.