Cloning of the nupC gene of Escherichia coli encoding a nucleoside transport system, and identification of an adjacent Insertion element, IS 186

Molecular Microbiology

Volumn 11, Issue 6, 1994, Pages 1159-1168

  Craig, Jane E ^a   Zhang, Yianbiao ^a   Gallagher, Maurice P ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; DNA SEQUENCE; ESCHERICHIA COLI; GENE INSERTION; GENE STRUCTURE; MOLECULAR CLONING; NONHUMAN; NUCLEOSIDE TRANSPORT; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CLONING, MOLECULAR; COMPARATIVE STUDY; CYCLIC AMP RECEPTOR PROTEIN; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; ESCHERICHIA COLI; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; NUCLEOSIDES; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SUPPORT, NON-U.S. GOV'T; TERMINATOR REGIONS (GENETICS);

ESCHERICHIA COLI;

EID: 0028272006     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1994.tb00392.x     Document Type: Article

References (50)

1. Completion maps of IS1, IS2, IS3, IS4, IS5, IS30 and IS150 locations in Escherichia coli K12
2. Completion of the IS map in E. coli: IS186 positions on the E. coli K12 chromosome
3. Construction and characterisation of a new cloning vehicle II. A multipurpose cloning system
4. A computer algorithm for testing potential prokaryotic terminator sequences
5. A vector for the construction of translational fusion to TEM β‐lactamase and the analysis of protein export signals and membrane protein topology
6. Sequence of the lactose permease gene
7. Structural analysis of a new GC‐specific insertion element IS186
8. Genomic sequencing
9. Cyclic AMP receptor protein: role in transcription activation
10. Epitopes of monoclonal antibodies which inhibit ubiquinol oxidase activity of the Escherichia coli cytochrome d complex localise a functional domain
11. A technique for radiolabeiling DNA restriction endonuclease fragments to high specific activity
12. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity—Addendum
13. Identification and localisation of the membrane‐associated, ATP‐binding subunit of the oligopeptide permease of Salmonella typhimurium
14. Isolation of nucleoside permeases from Escherichia coli
15. Sequence diversity among related genes for recognition of specific targets in DNA molecules
16. Multiple regulation of nucleoside catabolising enzymes: regulation of the deo operon by the cytR and deoR gene products
17. Phage T6‐colicin K receptor and nucleoside transport in Escherichia coli
18. The cAMP‐CRP/CytR nucleoprotein complex in Escherichia coli: two pairs of closely linked binding sites for the cAMP‐CRP activator complex are involved in combinatorial regulation of the cdd promoter
19. β‐galactoside transport in Escherichia coli: a functional dissection of lac permease
20. Visualisation of antigenic proteins on Western blots
21. The physical map of the whole Escherichia coli chromosome; application of a new strategy for rapid analysis and sorting of a large genomic library
22. Mechanism of action of showdomycin
23. A showdomycin‐resistant mutant of Escherichia coli K‐12 with altered nucleoside transport character
24. IS186; an Escherichia coli Insertion element Isolated from a cDNA library
25. Cleavage of structural proteins during assembly of bacteriophage T4
26. In vitro biochemical studies on translocation of presecretory proteins across the cytoplasmic membrane of Escherichia coli
27. Nucleoside transport systems in Escherichia coli K‐12. Specificity and regulation
28. Nucleoside transport in cells and membrane vesicles from Escherichia coli K‐12
29. Transport of pyrimidine nucleosides in ceils of Escherichia coli K‐12
30. Membrane topology of the integral membrane components, OppB and OppC, of the oligopeptide permease of Salmonella typhimurium
31. Improved tools for biological sequence comparison
32. Nucleoside and nucleobase uptake in cultured animal cells
33. Identification of the nucleotide sequence recognized by the cAMP‐CRP dependent CytR repressor protein in the deoP2 promoter in E. coli
34. Genome Organisation
35. Transport of purines and deoxyadenosine in Escherichia coli
36. Direct cloning and sequence analysis of enzymatically amplified genomic sequences
37. Terminal inverted repeats of prokaryotic transposable element IS186 which can generate duplications of variable length at an identical target sequence
38. Lambda ZAP; a bacteriophage lambda expression vector with in vivo excision properties
39. Preparation of phage plate stocks
40. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli
41. The bi‐directional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl paper
42. Use of T7 RNA polymerase to direct the expression of cioned genes
43. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes
44. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets; procedure and some applications
45. Design of cAMP‐CRP‐activated promoters in Escherichia coli
46. Location of the nupC gene on the physical map of Escherichia coli K‐12