Use of porous alginate sponges for substantial chondrocyte expansion and matrix production: Effects of seeding density

Biotechnology Progress

Volumn 24, Issue 2, 2008, Pages 452-457

  Yen, Chi Nan ^a,b   Lin, Yu Ru ^b   Chang, Margaret Dah Tsyr ^a   Tien, Chun Wen ^a   Wu, Yu Chih ^b   Liao, Chun Jen ^b   Hu, Yu Chen ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

CARTILAGE; CELL CULTURE; CELLS; POROUS MATERIALS;

CHONDROCYTES; POROUS ALGINATE;

ALGINATE;

ALGINIC ACID;

ANIMAL; ARTICLE; CARTILAGE; CARTILAGE CELL; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CHEMISTRY; CYTOLOGY; EXTRACELLULAR MATRIX; GROWTH, DEVELOPMENT AND AGING; IMMUNOHISTOCHEMISTRY; JOINT; METABOLISM; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCANNING ELECTRON MICROSCOPY; SWINE; TISSUE ENGINEERING; TISSUE SCAFFOLD; ULTRASTRUCTURE;

ALGINATES; ANIMALS; CARTILAGE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; CHONDROCYTES; EXTRACELLULAR MATRIX; IMMUNOHISTOCHEMISTRY; JOINTS; MICROSCOPY, ELECTRON, SCANNING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SWINE; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

SUS;

EID: 42049122309     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0702828     Document Type: Article

References (29)

1. Brittberg, M.; Lindahl, A.; Nilsson, A.; Ohlsson, C.; Isaksson, O.; Peterson, L. Treatment of Deep Cartilage Defects in the Knee with Autologous Chondrocyte Transplantation. N. Engl. J. Med. 1994, 331, 889-895.
2. Benya, P. D.; Shaffer, J. D. Dedifferentiated Chondrocytes Re-Express the Differentiated Collagen Phenotype When Cultured in Agarose Gels. Cell 1982, 30, 215-224.
3. Temenoff, J. S.; Mikos, A. G. Review: Tissue Engineering for Regeneration of Articular Cartilage. Biomaterials 2000, 21, 431-440.
4. Bonaventure, J.; Kadhom, N.; Cohen-Solal, L.; Ng, K. H.; Bourguignon, J.; Lasselin, C.; Freisinger, P. Reexpression of Cartilage-Specific Genes by Dedifferentiated Human Articular Chondrocytes Cultured in Alginate Beads. Exp. Cell Res. 1994, 212, 97-104.
5. Shapiro, L.; Cohen, S. Novel Alginate Sponges for Cell Culture and Transplantation. Biomaterials 1997, 18, 583-590.
6. Zmora, S.; Glicklis, R.; Cohen, S. Tailoring the Pore Architecture in 3-D Alginate Scaffolds by Controlling the Freezing Regime During Fabrication. Biomaterials 2002, 23, 4087-4094.
7. Dar, A.; Shachar, M.; Leor, J.; Cohen, S. Optimization of Cardiac Cell Seeding and Distribution in 3D Porous Alginate Scaffolds. Biotechnol. Bioeng. 2002, 80, 305-312.
8. Glicklis, R.; Shapiro, L.; Agbaria, R.; Merchuk, J. C.; Cohen, S. Hepatocyte Behavior within Three-Dimensional Porous Alginate Scaffolds. Biotechnol. Bioeng. 2000, 67, 344-353.
9. Gerecht-Nir, S.; Cohen, S.; Ziskind, A.; Itskovitz-Eldor, J. Three-Dimensional Porous Alginate Scaffolds Provide a Conducive Environment for Generation of Well-Vascularized Embryoid Bodies from Human Embryonic Stem Cells. Biotechnol. Bioeng. 2004, 88, 313-320.
10. Yen, C.-N. Chondrocyte Culture Using Porous Alginate Scaffolds, Cell Expansion and Maintenance of Differentiation. National Tsing Hua University, Hsinchu, 2006.
11. Kim, Y. J.; Sah, R. L. Y.; Doong, J. Y. H.; Grodzinsky, A. J. Fluorometric Assay of DNA in Cartilage Expiants Using Hoechst 33258. Anal. Biochem. 1988, 174, 168-176.
12. Enobakhare, B. O.; Bader, D. L.; Lee, D. A. Quantification of Sulfated Glycosaminoglycans in Chondrocyte/Alginate Cultures, by Use of 1,9-Dimethylmethylene Blue. Anal. Biochem. 1996, 243, 189-191.
13. Chomczynski, P.; Sacchi, N. Single-Step Method of Rna Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction. Anal. Biochem. 1987, 162, 156-159.
14. Chen, H.-C.; Lee, H.-P.; Ho, Y.-C.; Sung, M.-L.; Hu, Y.-C. Combination of Baculovirus-Mediated Gene Transfer and Rotating-Shaft Bioreactor for Cartilage Tissue Engineering. Biomaterials 2006, 27, 3154-3162.
15. Freed, L. E.; Hollander, A. P.; Martin, I.; Barry, J. R.; Langer, R.; Vunjak-Novakovic, G. Chondrogenesis in a Cell-Polymer-Bioreactor System. Exp. Cell Res. 1998, 240, 58-65.
16. Chen, Y.-L.; Lee, H.-P.; Chan, H.-Y.; Sung, L.-Y.; Chen, H.-C.; Hu, Y.-C. Composite Chondroitin-6-Sulfate/Dermatan Sulfate/Chitosan Scaffolds for Cartilage Tissue Engineering. Biomaterials 2007, 28, 2294-2305.
17. Rowley, J. A.; Madlambayan, G.; Mooney, D. J. Alginate Hydrogels as Synthetic Extracellular Matrix Materials. Biomaterials 1999, 20, 45-53.
18. Lee, D. A.; Reisler, T.; Bader, D. L. Expansion of Chondrocytes for Tissue Engineering in Alginate Beads Enhances Chondrocytic Phenotype Compared to Conventional Monolayer Techniques. Acta Orthop. Scand. 2003, 74, 6-15.
19. Wiseman, M.; Bader, L.; Reisler, T.; Lee, D. A. Passage in Monolayer Influences the Response If Chondrocytes to Dynamic Compression. Biorheology 2004, 41, 283-298.
20. Shakibaei, M.; de Souza, P.; Merker, H. J. Integrin Expression and Collagen Type Ii Implicated in Maintenance of Chondrocyte Shape in Monolayer Culture, An Immunomorphological Study. Cell Biol. Int. 1997, 21, 115-125.
21. Kino-Oka, M.; Yashiki, S.; Ota, Y.; Mushiaki, Y.; Sugawara, K.; Yamamoto, T.; Takezawa, T.; Taya, M. Subculture of Chondrocytes on a Collagen Type I-Coated Substrate with Suppressed Cellular Dedifferentiation. Tissue Eng. 2005, 11, 597-608.
22. Loredo, G. A.; Koolpe, M.; Benton, H. P. Influence of Alginate Polysaccharide Composition and Culture Conditions on Chondrocytes in Three-Dimensional Culture. Tissue Eng. 1996, 2, 115-125.
23. Chen, H.-C.; Lee, H.-P.; Sung, M.-L.; Liao, C.-J.; Hu, Y.-C. A Novel Rotating-Shaft Bioreactor for Two-Phase Cultivation of Tissue-Engineered Cartilage. Biotechnol. Prog. 2004, 20, 1802-1809.
24. Cho, S. H.; Oh, S. H.; Lee, J. H. Fabrication and Characterization of Porous Alginate/Polyvinyl Alcohol Hybrid Scaffolds for 3d Cell Culture. J. Biomater. Sci., Polym. Ed. 2005, 16, 933-947.
25. Li, Z. S.; Zhang, M. Q. Chitosan-Alginate as Scaffolding Material for Cartilage Tissue Engineering. J. Biomed. Mater. Res. 2005, 75A, 485-493.
26. Davisson, T.; Sah, R. L.; Ratcliffe, A. Perfusion Increases Cell Content and Matrix Synthesis in Chondrocyte Three-Dimensional Cultures. Tissue Eng. 2002, 8, 807-816.
27. Tallheden, T.; Karlsson, C.; Brunner, A.; van der Lee, J.; Hagg, R.; Tommasini, R.; Lindahl, A. Gene Expression During Redifferentiation of Human Articular Chondrocytes. Osteoarthritis Cartilage 2004, 12, 525-535.
28. Guo, J. F.; Jourdian, G. W.; Maccallum, D. K. Culture and Growth Characteristics of Chondrocytes Encapsulated in Alginate Beads. Connect. Tissue Res. 1989, 19, 277-297.
29. Masuda, K.; Sah, R. L.; Hejna, M. J.; Thonar, E. J. A Novel Two-Step Method for the Formation of Tissue-Engineered Cartilage by Mature Bovine Chondrocytes: The Alginate-Recovered-Chondrocyte (Arc) Method. J. Orthop. Res. 2003, 21, 139-148.