PCR ligation mutagenesis in transformable streptococci: Application and efficiency

Journal of Microbiological Methods

Volumn 49, Issue 2, 2002, Pages 193-205

  Lau, Peter C Y ^a   Sung, Chang Kyoo ^b   Lee, Janet H ^a   Morrison, Donald A ^b   Cvitkovitch, Dennis G ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Gene deletion; Genetic transformation; Mutagenesis; Oral streptococci; PCR

Indexed keywords

ANALYTIC METHOD; ARTICLE; BACTERIUM TRANSFORMATION; CONTROLLED STUDY; DIGESTION; DNA FLANKING REGION; DNA MODIFICATION; GENE CONSTRUCT; GENE DELETION; GENE SEQUENCE; GENE TARGETING; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; GENOME; LIGATION; MUTAGENESIS; MUTATION; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRODUCTIVITY; SEQUENCE HOMOLOGY; STREPTOCOCCUS;

DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; MUTAGENESIS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; STREPTOCOCCUS MUTANS; STREPTOCOCCUS PNEUMONIAE; TRANSFORMATION, GENETIC;

STREPTOCOCCUS; STREPTOCOCCUS MUTANS; STREPTOCOCCUS PNEUMONIAE;

EID: 0036164801     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(01)00369-4     Document Type: Article

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