Development and application of a stable HeLa cell line capable of site-specific transgenesis using the Cre-lox system: Establishment and application of a stable TNFRI knockdown cell line to cytotoxicity assay

Toxicology in Vitro

Volumn 22, Issue 4, 2008, Pages 1077-1087

  Saito, Fumiyo ^a,b   Yokota, Hirofumi ^a   Sudo, Yoshihisa ^a   Yakabe, Yoshikuni ^a   Takeyama, Haruko ^b   Matsunaga, Tadashi ^b  

^a CHEMICALS EVALUATION AND RESEARCH INSTITUTE   (Japan)

^b TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Cytotoxicity assay; Knock down; Site specific transgenesis; Stable cell line; TNF TNFRI

Indexed keywords

CRE RECOMBINASE; GREEN FLUORESCENT PROTEIN; TUMOR NECROSIS FACTOR RECEPTOR 1; XENOBIOTIC AGENT;

ARTICLE; CHROMOSOME 9; CHROMOSOME ARM; CONTROLLED STUDY; CRYOPRESERVATION; CYTOTOXICITY TEST; FEASIBILITY STUDY; FEMALE; GENE CASSETTE; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSCRIPTION; HELA CELL; HUMAN; HUMAN CELL; KNOCKOUT GENE; MOLECULAR CLONING; RNA INTERFERENCE; TOXICOLOGY; TRANSGENICS;

CELL LINE; GENE EXPRESSION; GENE SILENCING; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; INTEGRASES; PLASMIDS; RECEPTORS, TUMOR NECROSIS FACTOR, TYPE I; RNA INTERFERENCE; TOXICITY TESTS; XENOBIOTICS;

MAMMALIA;

EID: 41949141694     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2008.01.019     Document Type: Article

References (43)

1. Ahn S.Y., Cho C.H., Park K.G., Lee H.J., Lee S., Park S.K., Lee I.K., and Koh G.Y. Tumor necrosis factor-alpha induces fractalkine expression preferentially in arterial endothelial cells and mithramycin A suppresses TNF-alpha-induced fractalkine expression. Am. J. Pathol. 164 5 (2004) 1663-1672
2. Araki K., Araki M., and Yamamura K. Targeted integration of DNA using mutant lox sites in embryonic stem cells. Nucleic Acids Res. 25 (1997) 868-872
3. Berns K., Hijmans E.M., Mullenders J., Brummelkamp T.R., Velds A., Heimerikx M., Kerkhoven R.M., Madiredjo M., Nijkamp W., Weigelt B., Agami R., Ge W., Cavet G., Linsley P.S., Beijersbergen R.L., and Bernards R. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428 (2004) 427-431
4. Bouhassira E.E., Westerman K., and Leboulch P. Transcriptional behavior of LCR enhancer elements integrated at the same chromosomal locus by recombinase-mediated cassette exchange. Blood 90 (1997) 3332-3344
5. Bronson S.K., Plaehn E.G., Kluckman K.D., Hagaman J.R., Maeda N., and Smithies O. Single-copy transgenic mice with chosen-site integration. Proc. Natl. Acad. Sci. USA 93 (1996) 9067-9072
6. Dempsey P.W., Doyle S.E., He J.Q., and Cheng G. The signaling adaptors and pathways activated by TNF superfamily. Cytokine Growth Factor Rev. 14 (2003) 193-209
7. Djurovic S., Iversen N., Jeansson S., Hoover F., and Christensen G. Comparison of nonviral transfection and adeno-associated viral transduction on cardiomyocytes. Mol. Biotechnol. 28 (2004) 21-31
8. Duverger V., Murphy A.M., Sheehan D., England K., Cotter T.G., Hayes I., and Murphy F.J. The anticancer drug mithramycin A sensitises tumour cells to apoptosis induced by tumour necrosis factor (TNF). Brit. J. Cancer 90 (2004) 2025-2031
9. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
10. Eszterhas S.K., Bouhassira E.E., Martin D.I., and Fiering S. Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position. Mol. Cell Biol. 22 (2002) 469-479
11. Feng Y.Q., Seibler J., Alami R., Eisen A., Westerman K.A., Leboulch P., Fiering S., and Bouhassira E.E. Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange. J. Mol. Biol. 292 (1999) 779-785
12. Feng Y.Q., Lorincz M.C., Fiering S., Greally J.M., and Bouhassira E.E. Position effects are influenced by the orientation of a transgene with respect to flanking chromatin. Mol. Cell Biol. 21 (2001) 298-309
13. Goetze S., Baer A., Winkelmann S., Nehlsen K., Seibler J., Maass K., and Bode J. Performance of genomic bordering elements at predefined genomic loci. Mol. Cell Biol. 25 (2005) 2260-2272
14. Heggestad A.D., Notterpek L., and Fletcher B.S. Transposon-based RNAi delivery system for generating knockdown cell lines. Biochem. Biophys. Res. Commun. 316 (2004) 643-650
15. Iapalucci-Espinoza S., and Franze-Fernandez M.T. Effect of protein synthesis inhibitors and low concentrations of actinomycin D on ribosomal RNA synthesis. FEBS Lett. 107 (1979) 281-284
16. Ishiyama M., Miyazono Y., Sasamoto K., Ohkura Y., and Ueno K. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta 44 7 (1997) 1299-1305
17. Kanda N., and Watanabe S. Suppressive effects of antimycotics on tumor necrosis factor-alpha-induced CCL27, CCL2, and CCL5 production in human keratinocytes. Biochem. Pharmacol. 72 4 (2006) 463-473
18. Kim N.S., and Lee G.M. Response of recombinant Chinese hamster ovary cells to hyperosmotic pressure: effect of Bcl-2 overexpression. J. Biotechnol. 95 (2002) 237-248
19. Kleinjan D.J., and Van Heyningen V. Position effect in human genetic disease. Human Mol. Genet. 7 (1998) 1611-1618
20. Langer S.J., Ghafoori A.P., Byrd M., and Leinwand L. A genetic screen identifies novel non-compatible loxP sites. Nucleic Acids Res. 30 (2002) 3067-3077
21. Locardi C., Petrini C., Boccoli G., Testa U., Dieffenbach C., Butto S., and Belardelli F. Increased human immunodeficiency virus (HIV) expression in chronically infected U937 cells upon in vitro differentiation by hydroxyvitamin D3: roles of interferon and tumor necrosis factor in regulation of HIV production. J. Virol. 64 12 (1990) 5874-5882
22. Luo D., and Saltzman W.M. Synthetic DNA delivery systems. Nature Biotechnol. 18 (2000) 33-37
23. Miyagishi M., Matsumoto S., and Taira K. Generation of an shRNAi expression library against the whole human transcripts. Virus Res. 102 (2004) 117-124
24. Nagy B., Yeh W.C., Mak T.W., Chiu S.M., and Separovic D. FADD null mouse embryonic fibroblasts undergo apoptosis after photosensitization with the silicon phthalocyanine Pc4. Arch. Biochem. Biophys. 385 (2001) 194-202
25. Paddison P.J., Caudy A.A., and Hannon G.J. Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA 99 (2002) 1443-1448
26. Perry R.P., and Kelley D.E. Inhibition of RNA synthesis by actinomycin D: characteristic dose-response of different RNA species. J. Cell Physiol. 76 (1970) 127-139
27. Perucho M., Hanahan D., Lipsich L., and Wigler M. Isolation of the chicken thymidine kinase gene by plasmid rescue. Nature 285 (1980) 207-210
28. Saile B., Matthes N., El Armouche H., Neubauer K., and Ramadori G. The Bcl, NFkappaB and p53/p21WAF1 systems are involved in spontaneous apoptosis and in the anti-apoptotic effect of TGF-beta or TNF-alpha on activated hepatic stellate cells. Eur. J. Cell Biol. 80 (2001) 554-561
29. Saito F., Yokota H., Sudo Y., Yakabe Y., Takeyama H., and Matsunaga T. Application of RNAi inducible TNFRI knockdown cells to the analysis of TNFalpha-induced cytotoxicity. Toxicol. In Vitro 20 8 (2006) 1343-1353
30. Sastry M., and Patel D.J. Solution structure of the mithramycin dimer-DNA complex. Biochemistry 32 (1993) 6588-6604
31. Sauer B., and Henderson N. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc. Natl. Acad. Sci. USA 85 (1988) 5166-5170
32. Shimizu T., Oishi T., Omori A., Sugiura A., Hirota K., Aoyama H., Saito T., Sugaya T., Kon Y., Engel J.D., Fukamizu A., and Tanimoto K. Identification of cis-regulatory sequences in the human angiotensinogen gene by transgene coplacement and site-specific recombination. Mol. Cell Biol. 25 (2005) 2938-2945
33. Su Y., Liebhaber S.A., and Cooke N.E. The human growth hormone gene cluster locus control region supports position-independent pituitary- and placenta-specific expression in the transgenic mouse. J. Biol. Chem. 275 (2000) 7902-7909
34. Thorburn A. Death receptor-induced cell killing. Cell. Signal 16 (2004) 139-144
35. Tucci M.A., and Benghuzzi H.A. Quantitative analysis of the effect of surface impregnation with biomedical polymers on the proliferation rate of HeLa cells in culture. Biomed. Sci. Instrum. 31 (1995) 135-140
36. Urabe M., Kogure K., Kume A., Sato Y., Tobita K., and Ozawa K. Positive and negative effects of adeno-associated virus Rep on AAVS1-targeted integration. J. Genet. Virol. 84 (2003) 2127-2132
37. Van Antwerp D.J., Martin S.J., Verma I.M., and Green D.R. Inhibition of TNF-induced apoptosis by NF-kappaB. Trends Cell. Biol. 8 (1998) 107-111
38. Vowels B.R., Yang S., and Leyden J.J. Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes: implications for chronic inflammatory acne. Infect Immun. 63 8 (1995) 3158-3165
39. Warncke M., Vogt B., Ulrich J., von Laer M.D., Beyer W., Klump H., Micheel B., and Sheriff A. Efficient in vitro transduction of naive murine B cells with lentiviral vectors. Biochem. Biophys. Res. Commun. 318 (2004) 673-679
40. Wong E.T., Kolman J.L., Li Y.C., Mesner L.D., Hillen W., Berens C., and Wahl G.M. Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Nucleic Acids Res. 33 (2005) e147
41. Yahata K., Kishine H., Sone T., Sasaki Y., Hotta J., Chesnut J.D., Okabe M., and Imamoto F. Multi-gene gateway clone design for expression of multiple heterologous genes in living cells: conditional gene expression at near physiological levels. J. Biotechnol. 118 (2005) 123-134
42. Yaoita M., Ikariyama Y., and Aizawa M. Electrical effects on the proliferation of living HeLa cells cultured on optically transparent electrode surface. J. Biotechnol. 14 (1990) 321-332
43. Yurkow E.J., and Laskin J.D. Characterization of a photoalkylated psoralen receptor in HeLa cells. J. Biol. Chem. 262 (1987) 8439-8442