A bioassay for metals utilizing a human cell line

Toxicology in Vitro

Volumn 22, Issue 4, 2008, Pages 1025-1031

  Shea, J ^a   Moran, T ^b   Dehn, P F ^a  

^a CANISIUS COLLEGE   (United States)

^b Pollutech EnviroQuatics Ltd   (Canada)

Author keywords

Biomakers; HepG2; Human bioassay; Metal mixtures; Metallothionein

Indexed keywords

BIOLOGICAL MARKER; CADMIUM; HEMOGLOBIN; LEAD; MERCURY; METAL DERIVATIVE; METALLOTHIONEIN;

ARTICLE; ATOMIC EMISSION SPECTROMETRY; BINDING AFFINITY; BIOASSAY; CELL LINE; CELL STRAIN HEPG2; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CONTAMINATION; CONTROLLED STUDY; CORRELATION ANALYSIS; HUMAN; HUMAN CELL; MOLECULAR INTERACTION; PH MEASUREMENT; SEDIMENT; STATISTICAL SIGNIFICANCE;

BIOLOGICAL ASSAY; CADMIUM; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; ENVIRONMENTAL MONITORING; FRESH WATER; GEOLOGIC SEDIMENTS; HUMANS; LEAD; LIVER NEOPLASMS; MERCURY; METALLOTHIONEIN; METALS, HEAVY; SPECTROPHOTOMETRY, ATOMIC; WATER POLLUTANTS, CHEMICAL;

EID: 41949083582     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2008.02.014     Document Type: Article

References (40)

1. Allen S., Shea J.M., Felmet T., Gadra J., and Dehn P.F. A kinetic microassay for glutathione in cells plated on 96-well microtiter plates. Meth. Cell Sci. 22 (2001) 305-312
2. Bae D.S., Gennings C., Carter W.H., Yang R.S.H., and Campain J.A. Toxicological interactions among arsenic, cadmium, chromium, and lead in human keratinocytes. Toxicol. Sci. 63 (2001) 132-142
3. Bebianno M.J., Cravo A., Miguel C., and Morais S. Metallothionein concentrations in a population of Patella aspera: variation with size. Sci. Total Environ. 301 (2003) 151-161
4. Campana O., Sarasquete C., and Blasco J. Effect of lead on ALA-D activity, metallothionein levels, and lipid peroxidation in blood, kidney, and liver of the toadfish Halobatrachus didactylus. Ecotoxicol. Environ. Saf. 55 (2003) 116-125
5. Carvalho C.S., de Araujo H.S.S., and Fernandes M.N. Hepatic metallothionein in a teleost (Prochilodus scrofa) exposed to copper at pH 4.5 and pH 8.0. Comp. Biochem. Physiol. 137B (2004) 225-234
6. Dehn P.F., White C.M., Conners D.E., Shipkey G., and Cumbo T.A. Characterization of the human hepatocellular carcinoma (HepG2) cell line as an in vitro model for cadmium toxicity studies. In Vitro Cell. Devlop. Biol. 40 (2004) 172-182
7. Dehn P.F., Allen-Mocherie S., Karek J., and Thenappan A. Organochlorine insecticides: impacts on human HepG2 cytochrome P4501A, 2B activities and glutathione levels. Toxicol. In Vitro 19 (2005) 261-273
8. Domouhtsidou G.P., Dailianis S., Kaloyianni M., and Dimitriadis V.K. Lysosomal membrane stability and metallothionein content in Mytilllus galloprovincialis (L), as biomarkers - combination with trace metal concentrations. Mar. Pollut. Bull. 48 (2004) 572-586
9. Eaton D.L., and Cherian M.G. Determination of metallothionein in tissues by cadmium-hemoglobin affinity assay. In: Riordan J.F., and Vallee B.L. (Eds). Methods in Enzymology vol. 205 (1991), Academic Press, New York 83-88
10. Eaton D.L., and Toal B.F. Evaluation of the Cd/hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues. Toxicol. Appl. Pharmacol. 66 (1982) 134-142
11. Fulladosa E., Delmas F., Jun L., Villaescusa I., and Murat J.C. Cellular stress induced in cultured human cells by exposure to sludge extracts from water treatment plants. Ecotox. Environ. Saf. 53 (2002) 134-140
12. Garrison P.M., Tullis K., Aarts J.M.M.J.G., Brouwer A., Giesy J.P., and Denison M.S. Species-specific recombinant cell lines as bioassay systems for the detection of 2,3,7,8-tetrachlorodibenzo-p-dioxin-like chemicals. Fund. Appl. Toxicol. 30 (1996) 194-203
13. Geret F., Jouan A., Turpin V., Bebianno M.J., and Cosson R.P. Influence of metal exposure on metallothionein synthesis and lipid peroxidation in two bivalve mollusks: the oyster (Crassostrea gigas) and the mussel (Mytilus edulis). Aqaut. Living Resour. 15 (2002) 61-66
14. Giesy J.P., Hilscherova K., Jones P.D., Kannan K., and Machala M. Cell bioassays for detection of aryl hydrocarbon (AhR) and estrogen receptor (ER) mediated activity in environmental samples. Mar. Pollut. Bull. 45 (2002) 3-16
15. Haq F., Mahoney M., and Koropatnick J. Signaling events for metallothionein induction. Mutat. Res. 533 (2003) 211-226
16. Jahroudi N., Foster R., Price-Haughey J., Beitel G., and Gedamu L. Cell-type specific and differential regulation of human metallothionein genes. J. Biol. Chem. 265 (1990) 6506-6511
17. Jimenez I., Aracena P., Letelier M.E., Navarro P., and Speisky H. Chronic exposure of HepG2 cells to excess copper results in depletion of glutathione and induction of metallothionein. Toxicol. In Vitro 16 (2002) 167-175
18. Kagi J.H.R. Evolution, structure and chemical activity of class I metallothioneins: an overview. In: Suzuki K.T., Imura N., and Kimura M. (Eds). Metallothionein III. Biological Roles and Medical Implications (1993), Birkhauser Verlag, Berlin 29-56
19. Klaassen C.D., and Liu J. Role of metallothionein in cadmium-induced hepatotoxicity and nephrotoxicity. Drug Metab. Rev. 29 (1997) 79-102
20. Klaassen C.D., Liu J., and Choudhuri S. Metallothionein: an intracellular protein to protect against cadmium toxicity. Annu. Rev. Pharmacol. Toxicol. 39 (1999) 267-294
21. Lecoeur S., Videmann B., and Berny Ph. Evaluation of metallothionein as a biomarker of single and combined Cd/Cu exposure in Dreissena polymorpha. Environ. Res. 94 (2004) 184-191
22. Lee K., Nagler J.J., Fournier M., Lebeuf M., and Cyr D.G. Toxicological characterization of sediments from Baie Des Anglais on the St. Lawrence estuary. Chemosphere 39 (1999) 1019-1035
23. Lu J., Jin T., Nordberg G., and Nordberg M. Metallothionein gene expression in peripheral lymphocytes from cadmium-exposed workers. Cell Stress Chaperon. 6 (2001) 97-104
24. Lukkari T., Taavitsainen M., Soimasuo M., Oikari A., and Haimi J. Biomarker responses of the earthworm Aporrectodea tuberculata to copper and zinc exposure: differences between populations with and without earlier metal exposure. Environ. Pollut. 129 (2004) 377-386
25. Martin, T.D., Brockhoff, C.A., Creed, J.T., EMMC methods workgroup, 1994. Method 200.7, determination of metals and trace elements in water and wastes by inductively coupled plasma-atomic emission spectroscopy. Revision 4.4. US EPA.
26. Miura N., Matsumoto Y., Miyairi S., Nishiyama S., and Naganuma A. Protective effects of triterpene compounds against cytotoxicity of cadmium in HepG2 cells. Mol. Pharmacol. 56 (1999) 1324-1328
27. Moran, T., Adams, D., Walker, K., Zajdlik, B., 1997. Lambton Industrial Society 1994/1995 St. Clair River sediment program defining spatial extent and environmental conditions. Final Report, Pollutech EnviroQuatics Limited, Point Edward, ON.
28. Moran, T., Houtby, J., Zajdlik, B., Kierstead, T., 2003. Sarnia Lambton Environmental Association 2001/2002 St. Clair River biological program - assessment of bioaccumulation/biomagnification potential and trends. Final Report, Pollutech EnviroQuatics Limited, Point Edward, ON, p. 112.
29. Perceval O., Pinel-Alloul B., Methot G., Couillard Y., Giguere A., Campbell P.G.C., and Hare L. Cadmium accumulation and metallothionein synthesis in freshwater bivalves (Pyganodon grandis): relative influence of the metal exposure gradient vs. limnological variability. Environ. Pollut. 118 (2002) 5-17
30. Perez M.J., and Cederbaum A.I. Metallothionein 2A induction by zinc protects HepG2 cells against CYP2E1-dependent toxicity. Free Rad. Biol. Med. 34 (2003) 443-455
31. Pieczonka C.M., and Dehn P.F. An automated end-point microassay for adenosine-5′-triphosphate using a microplate reader. J. Tissue Cult. Meth. 15 (1993) 210-215
32. Pollutech EnviroQuatics Ltd., 1999. St. Clair River sediment program: establishment of a reference condition.
33. Regoli F., Pellegrini D., Winston G.W., Gorbi S., Giuliani S., Virno-Lamberti C., and Bompadre S. Application of biomarkers for assessing the biological impact of dredged materials in the Mediterranean: the relationship between antioxidant responses and susceptibility to oxidative stress in the red mullet (Mullus barbatus). Mar. Pollu. Bull. 44 (2002) 912-922
34. r cells in response to lead treatment. Chem. Biol. Interact. 72 (1989) 347-361
35. Roper J.M., Cherry D.S., Simmers J.W., and Tatem H.E. Bioaccumulation of toxicants in the zebra mussel, Dreissena polymorpha, at the Times Beach Confined Disposal Facility, Buffalo, NY. Environ. Pollut. 94 (1996) 117-129
36. Shea, J., Moran, T., Dehn, P.F., 1999. HepG2 Bioassay for metals. Canadian Tech. Rep. of Fisheries and Aquatic Sciences No. 2293, 29.
37. Shelton, L.R., Capel, P.D., 1994. Guidelines for collecting and processing samples of stream bed sediment for analysis of trace elements and organic contaminants for the national water-quality assessment program. USGS Open-File Rep. 94-458, p. 20.
38. Urani C., Calini V., Melchioretto P., Morazzoni F., Canevali C., and Camatini M. Different induction of metallothioneins and Hsp70 and presence of the membrane transporter ZnT-1 in HepG2 cells exposed to copper and zinc. Toxicol. In Vitro 17 (2003) 553-559
39. Urani C., Melchioretto P., Canevali C., and Crosta G.F. Cytotoxicity and induction of protective mechanisms in HepG2 cells exposed to cadmium. Toxicol. In Vitro 19 (2005) 887-892
40. Urani C., Melchioretto P., Canevali C., Morazzoni F., and Gribaldo L. Metallothionein and hsp70 expression in HepG2 cells after prolonged cadmium exposure. Toxicol. In Vitro 21 (2007) 314-319