A kinetic microassay for glutathione in cells plated on 96-well microtiter plates

Methods in Cell Science

Volumn 22, Issue 4, 2000, Pages 305-312

  Allen, S ^a   Shea, J M ^a   Felmet, T ^a   Gadra, J ^a   Dehn, P F ^a  

^a CANISIUS COLLEGE   (United States)

Author keywords

Cellular toxicology; Glutathione; Hepatocytes

Indexed keywords

GLUTATHIONE; METAL; ORGANOCHLORINE PESTICIDE;

ARTICLE; CELL FREE SYSTEM; CELL LEVEL; CELL LINE; CELL STRAIN HEPG2; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; COST; CYTOTOXICITY; ENZYMATIC ASSAY; EXPOSURE; EXTRACTION; FILTER; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; KINETICS; MARINE ENVIRONMENT; MONITORING; PROTEIN ANALYSIS; REPRODUCIBILITY; SAMPLE; SEDIMENT;

CARCINOMA, HEPATOCELLULAR; DITHIONITROBENZOIC ACID; GLUTATHIONE; HUMANS; INSECTICIDES; LIVER NEOPLASMS; REPRODUCIBILITY OF RESULTS; SULFHYDRYL REAGENTS; TUMOR CELLS, CULTURED;

EID: 0034426765     PISSN: 13815741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1017585308255     Document Type: Article

References (7)

1. Methods for the selective modifications of glutathione metabolism and study of glutathione transport
2. Cytotoxicity measures: Choices and methods
3. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples
4. Sensitive enzymatic cycling assay for glutathione: Measurements of glutathione content and its modulation by buthionine sulfoximine in vivo and in vitro in human colon cancer
5. Enzymatic method for quantitative determination of nanogram amounts of total oxidized glutathione: Applications to mammalian blood and other tissues
6. Determination of glutathione and glutathione disulfide in biological samples